兰州大学机构库 >生命科学学院
The Mitochondrial Folylpolyglutamate Synthetase Gene Is Required for Nitrogen Utilization during Early Seedling Development in Arabidopsis
Jiang, Ling1,2; Liu, Yanyan1; Sun, Hong1; Han, Yueting1; Li, Jinglai3; Li, Changkun3; Guo, Wenzhu1,4; Meng, Hongyan1; Li, Sha1,5; Fan, Yunliu1,2; Zhang, Chunyi1,2
2013-02
Source PublicationPLANT PHYSIOLOGY   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN0032-0889
Volume161Issue:2Pages:971-989
AbstractInvestigations into the biochemical processes and regulatory mechanisms of nitrogen (N) utilization can aid in understanding how N is used efficiently in plants. This report describes a deficiency in N utilization in an Arabidopsis (Arabidopsis thaliana) transfer DNA insertion mutant of the mitochondrial folylpolyglutamate synthetase gene DFC, which catalyzes the conjugation of glutamate residues to the tetrahydrofolate during folate synthesis. The mutant seedlings displayed several metabolic changes that are typical of plant responses to low-N stress, including increased levels of starch and anthocyanin synthesis as well as decreased levels of soluble protein and free amino acid, as compared with those in wild-type seedlings when external N was sufficient. More striking changes were observed when dfc seedlings were grown under N-limited conditions, including shorter primary roots, fewer lateral roots, higher levels of glycine and carbon-N ratios, and lower N content than those in wild-type seedlings. Gene expression studies in mutant seedlings revealed altered transcript levels of several genes involved in folate biosynthesis and N metabolism. The biochemical and metabolic changes also suggested that N assimilation is drastically perturbed due to a loss of DFC function. The observation that elevated CO2 partly rescued the dfc phenotypes suggests that the alterations in N metabolism in dfc may be mainly due to a defect in photorespiration. These results indicate that DFC is required for N utilization in Arabidopsis and provide new insight into a potential interaction between folate and N metabolism.
Subject AreaPlant Sciences
PublisherAMER SOC PLANT BIOLOGISTS
DOI10.1104/pp.112.203430
Publication PlaceROCKVILLE
Indexed BySCIE
Language英语
First Inst
Funding Project国家重点基础研究发展计划以及国家重大科学研究计划(973计划)
Project NumberNational Basic Research Program of China [2013CB127003]
WOS IDWOS:000314360100030
Funding OrganizationMOST
SubtypeArticle
Department
[Jiang, Ling;
Liu, Yanyan;
Sun, Hong;
Han, Yueting;
Guo, Wenzhu;
Meng, Hongyan;
Li, Sha;
Fan, Yunliu;
Zhang, Chunyi] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China;
[Jiang, Ling...更多
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/115261
Collection生命科学学院
Corresponding AuthorZhang, Chunyi
Affiliation
1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
2.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
3.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
4.Huazhong Agr Univ, Wuhan 430070, Peoples R China
5.Lanzhou Univ, Lanzhou 730000, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Ling,Liu, Yanyan,Sun, Hong,et al. The Mitochondrial Folylpolyglutamate Synthetase Gene Is Required for Nitrogen Utilization during Early Seedling Development in Arabidopsis[J]. PLANT PHYSIOLOGY,2013,161(2):971-989.
APA Jiang, Ling.,Liu, Yanyan.,Sun, Hong.,Han, Yueting.,Li, Jinglai.,...&Zhang, Chunyi.(2013).The Mitochondrial Folylpolyglutamate Synthetase Gene Is Required for Nitrogen Utilization during Early Seedling Development in Arabidopsis.PLANT PHYSIOLOGY,161(2),971-989.
MLA Jiang, Ling,et al."The Mitochondrial Folylpolyglutamate Synthetase Gene Is Required for Nitrogen Utilization during Early Seedling Development in Arabidopsis".PLANT PHYSIOLOGY 161.2(2013):971-989.
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