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Fusion expression of Raphanus sativus-antifungal protein 1 (Rs-AFP1) in Escherichia coli and its antifungal activity on Verticillium dahliae
Zhou, XJ; Wang, LS(王仑山); Lin, ZP; Jia, JW; Lu, S; Chu, ZQ; Chen, XY; Chen, XY (reprint author), Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet,Inst Plant Physiol, Shanghai 200032, Peoples R China.
2000-07
Source PublicationACTA BOT SIN   Impact Factor & Quartile
ISSN0577-7496
Volume42Issue:7Pages:703-707
AbstractRaphanus sativus L. antifungal protein 1 (Rs-AFP1) gene was isolated by polymerase chain reaction (PCR). The complete open reading frame and the fragment encoding the putative mature protein were inserted into the prokaryotic expression vector pET-32b(+), respectively. Subsequent expression showed that the Rs-AFP1 was produced in E. coli as a 27 kD fusion protein only when the N-terminal signal peptide was removed. After treatment with thrombin to remove part of the N-terminal His.tag sequence, the bacterially expressed Rs-AFP1 was used for fungal growth inhibition assay which was conducted on Verticillium dahliae Kleb., a soil-born fungus causing the cotton wilt disease. Results showed that, in the liquid medium, the Rs-AFP1 fusion protein at a concentration of 0.3 g/L clearly inhibited the growth of V. dahliae and the germination of spores. Thus the bacterially expressed fusion protein had the antifungal activity against V. dahliae.
KeywordRaphanus sativus-antifungal protein 1 (Rs-AFP1) fungus Verticillium dahliae prokaryotic expression
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
PublisherSCIENCE CHINA PRESS
Publication PlaceBEIJING
Indexed BySCIE
Language中文
First Inst
Host of Journal中国植物学会 ; 中国科学院植物研究所
WOS IDWOS:000088663700009
SubtypeArticle
DepartmentChinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet,Inst Plant Physiol, Shanghai 200032, Peoples R China;
Lanzhou Univ, Coll Life Sci, Lanzhou 730000, Peoples R China
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Cited Times:0[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/115651
Collection生命科学学院
Corresponding AuthorChen, XY (reprint author), Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet,Inst Plant Physiol, Shanghai 200032, Peoples R China.
Recommended Citation
GB/T 7714
Zhou, XJ,Wang, LS,Lin, ZP,et al. Fusion expression of Raphanus sativus-antifungal protein 1 (Rs-AFP1) in Escherichia coli and its antifungal activity on Verticillium dahliae[J]. ACTA BOT SIN,2000,42(7):703-707.
APA Zhou, XJ.,Wang, LS.,Lin, ZP.,Jia, JW.,Lu, S.,...&Chen, XY .(2000).Fusion expression of Raphanus sativus-antifungal protein 1 (Rs-AFP1) in Escherichia coli and its antifungal activity on Verticillium dahliae.ACTA BOT SIN,42(7),703-707.
MLA Zhou, XJ,et al."Fusion expression of Raphanus sativus-antifungal protein 1 (Rs-AFP1) in Escherichia coli and its antifungal activity on Verticillium dahliae".ACTA BOT SIN 42.7(2000):703-707.
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