兰州大学机构库 >口腔医学院
Engineering Injectable Bone Using Bone Marrow Stromal Cell Aggregates
Ma, Dongyang1,2; Zhong, Cuiping3; Yao, Hong2; Liu, Yanpu1; Chen, Fulin4; Li, Jianxue2; Zhao, Jinlong1; Mao, Tianqiu1; Ren, Liling5
2011-06
Source PublicationSTEM CELLS AND DEVELOPMENT   Impact Factor & Quartile Of Published Year  The Latest Impact Factor & Quartile
ISSN1547-3287
Volume20Issue:6Pages:989-999
AbstractWith the increasing popularity of minimally invasive surgery, to develop an injectable bone would be highly preferable for the repair of bone nonunions and defects. However, the use of dissociated cells and exogenous carriers to construct injectable bone faces several drawbacks. To circumvent these limitations, we first harvested a cell sheet from rabbit bone marrow stromal cells using a continuous culture method and a scraping technique. The obtained sheet was then cut into fragments of multicellular aggregates, each of which was composed of a certain number of cells, extracellular matrix, and intercellular connections. The aggregates showed apparent mineralization properties, high alkaline phosphatase activity, increased osteocalcin content, and upregulated bone markers, implying their in vitro osteogenic potential. Then, serum-free medium (the control group), dissociated cell suspension (the cell group), and suspension of multicellular aggregates (the aggregate group) were injected subcutaneously on the back of the nude mice to evaluate ectopic bone formation. The results revealed that the aggregate group showed significantly larger and denser bone at the injection sites than the cell group, whereas bone formation did not occur in the control group. Additionally, when injecting them locally into the mandibular fracture gap of delayed healing in a rabbit model, we observed the most improved bone healing in the aggregate group. More cells survive and retain at the injection sites in the aggregate group than that in the cell group postoperatively. Our study indicates that the multicellular aggregates might be considered a promising strategy to generate injectable bone tissue and improve the efficacy of cell therapy.
Subject AreaCell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
PublisherMARY ANN LIEBERT INC
DOI10.1089/scd.2010.0348
Publication PlaceNEW ROCHELLE
Indexed BySCIE
Language英语
First Inst
WOS IDWOS:000291001000006
SubtypeArticle
Department
[Ma, Dongyang;
Liu, Yanpu;
Zhao, Jinlong;
Mao, Tianqiu] Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Xian 710032, Peoples R China;
[Ma, Dongyang;
Yao, Hong;
Li, Jianxue] Lanzhou Gen Hosp, Lanzhou Command PLA, Dept Oral & Maxillofacial Surg, Lanzhou, Peoples R China;
[Zhong, Cuiping] Lanzhou Gen Hosp, Lanzhou Command PLA, Dept Otolaryngol, Lanzhou, Peoples R China;
[Chen, Fulin] NW Univ Xian, Fac Life Sci, Dept Biosci, Rege Lab Tissue Engn, Xian 710069, Peoples R China;
[Ren, Liling] Lanzhou Univ, Sch Stomatol, Dept Orthodont, Lanzhou 730003, Peoples R China...更多
Citation statistics
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/122087
Collection口腔医学院
Corresponding AuthorMao, Tianqiu
Affiliation
1.Fourth Mil Med Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Xian 710032, Peoples R China
2.Lanzhou Gen Hosp, Lanzhou Command PLA, Dept Oral & Maxillofacial Surg, Lanzhou, Peoples R China
3.Lanzhou Gen Hosp, Lanzhou Command PLA, Dept Otolaryngol, Lanzhou, Peoples R China
4.NW Univ Xian, Fac Life Sci, Dept Biosci, Rege Lab Tissue Engn, Xian 710069, Peoples R China
5.Lanzhou Univ, Sch Stomatol, Dept Orthodont, Lanzhou 730003, Peoples R China
Recommended Citation
GB/T 7714
Ma, Dongyang,Zhong, Cuiping,Yao, Hong,et al. Engineering Injectable Bone Using Bone Marrow Stromal Cell Aggregates[J]. STEM CELLS AND DEVELOPMENT,2011,20(6):989-999.
APA Ma, Dongyang.,Zhong, Cuiping.,Yao, Hong.,Liu, Yanpu.,Chen, Fulin.,...&Ren, Liling.(2011).Engineering Injectable Bone Using Bone Marrow Stromal Cell Aggregates.STEM CELLS AND DEVELOPMENT,20(6),989-999.
MLA Ma, Dongyang,et al."Engineering Injectable Bone Using Bone Marrow Stromal Cell Aggregates".STEM CELLS AND DEVELOPMENT 20.6(2011):989-999.
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