兰州大学机构库 >第一临床医学院
中国汉族人群冠心病候选基因变异及其临床关联的地域特征
Alternative TitleGeographical characteristics of single nucleotide polymorphism of candidate genes associated with coronary artery disease in Chinese Han population
王胜煌; 崔翰斌; 王东琦; 陈晓敏; 张宏考; 崔长琮; 陈新义; 刘新宏; 张征; 白锋; Muhlestein JB
2008
Source Publication中华心血管病杂志   Impact Factor & Quartile
ISSN0253-3758
Volume36Issue:1Pages:24-29
Abstract目的探讨冠心病候选基因单核苷酸多态性在中国汉族人群中的地域分布特征。方法选择西安、十堰及宁波地区汉族人群中经冠状动脉造影证实的冠心病及正常对照为研究对象。采用苯酚-氯仿-异丙醇抽提外周血白细胞全基因组DNA,通过聚合酶链式反应(PCR)及限制性片段长度多态性(RFLP)结合测序方法确定候选基因单核苷酸多态性(SNP)。SNP位点选择ATP结合盒转运子(ABCA1-G596A),胆固醇酯转运蛋白(CETP-Taq1B),脂蛋白脂酶(LPL-HindⅢ及LPL-PvuⅡ)。结果入选西安、十堰及宁波三个地区中国
Other AbstractOBJECTIVE: To investigate the geographical characteristics of single nucleotide polymorphism (SNP) of candidate genes associated with coronary artery disease in Chinese Han population.METHODS: Study population were Chinese Han nationality recruited from Xi'an, Shiyan and Ningbo districts. Patients with coronary artery disease were defined by coronary angiography with stenosis >or= 50% and control subjects with stenosis < 10%, respectively. The DNA was extracted from peripheral white blood cell by approach comprised proteinase K digestion, phenol and chloroform extraction as well as isopropanol precipitation. The SNP of ATP-binding cassette transporter (ABCA1)-G596A, cholesteryl ester transfer protein (CETP)-Taq1B, Lipoprotein lipase (LPL)-Hind III and LPL-Pvu II were genotyped by PCR-RFLPs, and verified by gene sequencing.RESULTS: A Total of 615 patients undertaken coronary angiography were recruited from cardiac center in Xi'an (220), Ningbo (209) and Shiyan district (186), China (mean age 60 +/- 10 years, 75.9% males). Diabetes mellitus was more prevalent in Xi'an Cohort population than Shiyan and Ningbo cohort (P < 0.01). Plasma total cholesterol, LDL cholesterol and triglyceride levels in Xi'an Cohort population were significantly higher, and HDL-C siginificantly lower than in Shiyan and Ningbo cohort population [HDL-C: (1.17 +/- 0.48) mmol/L vs. (1.25 +/- 0.33) mmol/L and (1.29 +/- 0.44) mmol/L, P < 0.05]. Distribution differences for ABCA1-G596A and CETP-Taq1B genotypes were found in Xi'an Cohort population compared to Ningbo and Shiyan cohorts (for ABCA1, Xi'an: 0.24, 0.53, 0.23 and Shiyan: 0.17, 0.62, 0.21 and Ningbo: 0.34, 0.37, 0.29, for GG, AG, AA, respectively, P < 0.01; and for CETP, Xi'an: 0.29, 0.54, 0.17 and Shiyan: 0.38, 0.40, 0.22 and Ningbo: 0.39, 0.49, 0.12 for B1B1, B1B2, B2B2, respectively, P < 0.01), but not for LPL variants. ABCA1-G596A variant predicted HDL-C [Xi'an: (1.2 +/- 0.3) mmol/L, (1.3 +/- 0.2) mmol/L and (1.4 +/- 0.4) mmol/L, P = 0.01; Shiyan: (1.1 +/- 0.4) mmol/L: (1.2 +/- 0.3) mmol/L and (1.3 +/- 0.4) mmol/L, P = 0.03; Ningbo, (1.2 +/- 0.3) mmol/L, (1.3 +/- 0.4) mmol/L and (1.4 +/- 0.3) mmol/L, across GG, GA to AA genotype, respectively, P = 0.01] and TG levels [Xi'an: (2.4 +/- 1.3) mmol/L, (1.9 +/- 0.9) mmol/L and (1.6 +/- 0.8) mmol/L, P < 0.01; Shiyan: (2.1 +/- 1.0) mmol/L, (1.9 +/- 0.8) mmol/L and (1.8 +/- 0.7) mmol/L, P = 0.03; Ningbo: (1.9 +/- 1.1) mmol/L, (1.8 +/- 0.9) mmol/L and (1.6 +/- 0.7) mmol/L, across GG, GA to AA genotype, P = 0.05] with dose-dependent relationship. LPL-Hind III (+) carriers had higher triglycerides in three cohort population [Xi'an: (2.2 +/- 1.0) mmol/L, (1.8 +/- 0.9) mmol/L, (1.6 +/- 0.7) mmol/L, P = 0.01; Shiyan: (2.1 +/- 0.7) mmol/L, (1.9 +/- 1.0) mmol/L, (1.7 +/- 0.6) mmol/L, P = 0.01; Ningbo: (1.8 +/- 1.0) mmol/L, (1.6 +/- 0.6) mmol/L and (1.4 +/- 0.5) mmol/L, for +/+, +/- and -/- genotypes, respectively, P = 0.001]. SNP of CETP-Taq1B, LPL-Hind III and LPL-Pvu II predicted HDL-C and/or TG levels in different cohort population with different manners. All these SNP were not significantly associated with the development of coronary artery disease (all P > 0.05).CONCLUSION: A geographical heterogeneity of environmental and genetic risk factors related to the development of coronary artery disease exists in Chinese Han population. Irrespective of the different geographical cohort of Chinese Han population, the SNP of candidate genes can partly predict the differences in risk-related plasma HDL-C and/or TG levels rather than angiographic coronary artery disease.
Keyword冠状动脉疾病 ATP结合匣式转运子 脂蛋白脂酶 多态性,单核苷酸 地域因素
Subject AreaBiochemistry & Molecular Biology ; Geriatrics & Gerontology ; Anthropology ; Cardiovascular System & Cardiology ; Genetics & Heredity ; Geography
Publication PlaceBEIJING
Indexed ByPubMed ; MEDLINE ; BIOSIS ; CSCD
Language中文
First Inst
Host of Journal中华医学会
Project Number2003美国沙漠基金资助项目
CSCD IDCSCD:3208198
PMID 19099922
BIOSIS IDBIOSIS:PREV200800444198
IRIDCSCD:0000423
Department浙江省宁波市第一医院心脏内科;
陕西省人民医院心脏内科;
西安交通大学第一附属医院心脏内剩;
湖北省十堰市人民医院心脏内科;
兰州大学第一附属医院心脏内科;
美国犹他大学LDS医院
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/167012
Collection第一临床医学院
Recommended Citation
GB/T 7714
王胜煌,崔翰斌,王东琦,等. 中国汉族人群冠心病候选基因变异及其临床关联的地域特征[J]. 中华心血管病杂志,2008,36(1):24-29.
APA 王胜煌.,崔翰斌.,王东琦.,陈晓敏.,张宏考.,...&Muhlestein JB.(2008).中国汉族人群冠心病候选基因变异及其临床关联的地域特征.中华心血管病杂志,36(1),24-29.
MLA 王胜煌,et al."中国汉族人群冠心病候选基因变异及其临床关联的地域特征".中华心血管病杂志 36.1(2008):24-29.
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