兰州大学机构库 >第二临床医学院
siRNA沉默COX-2基因对骨肉瘤MG-63细胞凋亡和自噬的影响
陈秀锦; 杨明宣; 崔兆辉; 胡旭昌; 汪静; 安丽萍; 马靖琳; 王栓科
2014-05-25
Source Publication肿瘤   Impact Factor & Quartile
Volume34Issue:5Pages:425-429
Abstract目的 :探讨小干扰RNA(small interfering RNA,siRNA)抑制环氧合酶-2(cyclooxygenase-2,COX-2)基因的表达对骨肉瘤MG-63细胞凋亡和自噬的影响及其可能的机制。方法 :将靶向COX-2基因的siRNA(COX-2siRNA)转染至骨肉瘤MG-63细胞后,FCM法检测其对细胞凋亡的影响,实时荧光定量PCR法检测细胞中COX-2、Bcl-2、微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)和Beclin 1 mRNA的表达,蛋白质印迹法检测COX-2、Bcl-2和Becli...
Keyword骨肉瘤 RNA干扰 细胞凋亡 自噬 细胞,MG-63
Indexed ByCSCD
Language中文
First Inst
Funding Project甘肃省自然科学基金计划
Project Number甘肃省自然科学基金资助项目(编号:1010RJZA124)
CSCD IDCSCD:5147216
Funding OrganizationGSSTD
IRIDCNKI:0000566
Department兰州大学第二医院骨科;
甘肃省骨关节疾病研究重点实验室
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/175466
Collection第二临床医学院
Recommended Citation
GB/T 7714
陈秀锦,杨明宣,崔兆辉,等. siRNA沉默COX-2基因对骨肉瘤MG-63细胞凋亡和自噬的影响[J]. 肿瘤,2014,34(5):425-429.
APA 陈秀锦.,杨明宣.,崔兆辉.,胡旭昌.,汪静.,...&王栓科.(2014).siRNA沉默COX-2基因对骨肉瘤MG-63细胞凋亡和自噬的影响.肿瘤,34(5),425-429.
MLA 陈秀锦,et al."siRNA沉默COX-2基因对骨肉瘤MG-63细胞凋亡和自噬的影响".肿瘤 34.5(2014):425-429.
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