兰州大学机构库 >第二临床医学院
ELTD1、VEGF、DLL4在胃腺癌中的表达及 临床意义
Alternative TitleExpression and clinical significance of ELTD1, VEGF and DLL4 in gastric adenocarcinoma
魏友芬
Subtype硕士
Thesis Advisor李桂香
2018-03-10
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword胃癌 ELTD1 DLL4 VEGF 免疫组化
Abstract

目的:探讨ELTD1、VEGF、DLL4在胃腺癌中的表达及它们之间的相关性,同时阐明三者与肿瘤血管生成、肿瘤细胞增殖以及组织缺氧之间的关系,以期为评估胃癌生物学行为提供更好的生物学指标,为胃癌的治疗开辟新的靶点、提供新的理论依据。方法:应用免疫组化染色的方法检测43例胃腺癌组织、5例癌旁组织、4例慢性胃炎组织、7例健康人胃组织中ELTD1、DLL4、VEGF、CD34、CA9等几种蛋白的表达。同时统计各研究对象的临床病理参数及Ki-67阳性细胞数表达水平,运用SPSS23.0统计学软件进行统计学分析。结果:(1)胃癌组织中ELTD1的表达与胃癌的临床分期、淋巴结转移、肿瘤浸润深度、肿瘤组织分化程度无显著相关性(p>0.05)。(2)VEGF的表达与胃癌的临床分期、淋巴结转移、肿瘤浸润深度、组织分化程度显著相关(p<0.05)。(3)DLL4的表达与胃癌的临床分期、淋巴结转移、肿瘤浸润深度存在相关性(p<0.05),而与胃癌组织分化程度无相关性(p>0.05)。(4)胃癌组织中ELTD1的表达与VEGF、DLL4、CD34、CA9、Ki-67的表达均无相关性(p>0.05)。(5)VEGF在癌组织中的表达水平与CD34表达水平呈显著正相关(p<0.01,rs>0),与CA9表达水平呈负相关(p=0.01,rs<0),但与Ki-67表达无显著相关性(p>0.05)。(6)癌组织中DLL4表达水平与CD34蛋白表达水平呈正相关(p<0.01,rs>0),与CA9表达水平呈显著负相关(p<0.01,rs<0),而与Ki-67无相关性(p>0.05)。(7)ELTD1在胃癌组织、癌旁组织、慢性胃炎组织、正常胃黏膜组织等不同组织中表达无显著差异(p>0.05)。(8)在胃癌、癌旁、慢性胃炎、正常胃黏膜等4种不同组织中VEGF蛋白的表达存在显著差异(p<0.05),而将胃癌组织中蛋白的表达与其他几种组织之间蛋白的表达进行两两比较发现,胃癌组织中VEGF的表达与癌旁组织、慢性胃炎组织、正常胃黏膜组织之间均存在显著差异(p<0.05)。(9)在胃癌、癌旁、慢性胃炎、正常胃黏膜等4种不同组织中DLL4蛋白的表达存在显出差异(p<0.05),而将胃癌组织中蛋白的表达与其他几种组织之间蛋白的表达进行两两比较发现,胃癌组织中DLL4的表达与慢性胃炎组织、正常胃黏膜组织之间均存在显著差异(p< 0.05),而与癌旁组织之间不存在差异(p>0.05)。结论:(1)胃癌组织中ELTD1的表达与胃癌发生发展以及肿瘤组织的微血管密度、缺氧程度、细胞增殖情况均无相关性,未能证明ELTD1的表达受VEGF、DLL4的调控。ELTD1在胃癌中的表达及作用还有待进一步的研究。(2)DLL4、VEGF两个蛋白的表达水平均与胃恶性肿瘤的发生发展相关,并且二者的表达均与组织缺氧程度呈负相关,而与肿瘤组织微血管密度呈正相关,表明VEGF及DLL4是胃癌发生发展过程中的重要调控分子。

Other Abstract

Objective:To explore the expression of ELTD1, VEGF and DLL4 in gastric adenocarcinoma and their revelance, and clarify the relationship between ELTD1, VEGF and DLL4 with tumor angiogenesis, tumor cell proliferation and hypoxia, in order to provide a better biological indicator to evaluate the biological behavior of gastric cancer, and provide new targets for the treatment of GC and theoretical basis.Methods: Immunohistochemical staining was used to detect the expression of ELTD1, DLL4, VEGF, CD34, and CA9 in 43 cases of gastric adenocarcinoma tissues, 5 cases of paracancerous tissues, 4 cases of chronic gastritis tissues, and 7 cases of healthy human gastric tissues. Meanwhile, the clinicopathological parameters and expression levels of Ki-67 positive cells were recorded. SPSS23.0 statistical software was used for statistical analysis.Result: (1) There was no correlation between ELTD1 expression in gastric cancer tissues and the clinical stage, lymph node metastasis, depth of tumor invasion and histological differentiation of gastric cancer (p>0.05). (2) The expression of VEGF was significantly correlated with the clinical stage, lymph node metastasis, depth of tumor invasion and the degree of tissue differentiation in gastric cancer (p< 0.05). (3) The expression of DLL4 was correlated with the clinical stage, lymph node metastasis, and depth of tumor invasion (p<0.05), but not with the degree of differentiation of gastric cancer (p>0.05). (4) There was no correlation between the expression of ELTD1 in gastric carcinoma and the expression of VEGF, DLL4, CD34, CA9 and Ki-67 (p> 0.05). (5) The expression level of VEGF in cancer tissues was positively correlated with the expression level of CD34 (p< 0.01, rs > 0), and negatively correlated with the expression level of CA9 (p=0.01, rs < 0), but it had no significant correlation with Ki-67 (p> 0.05). (6) The expression level of DLL4 in cancer tissues was positively correlated with the expression level of CD34 protein (p< 0.01, rs > 0), and negatively correlated with the expression level of CA9 (p< 0.01, rs < 0), but not correlated with Ki-67 (p> 0.05). (7)There was no significant difference in the expression of ELTD1 in gastric cancer tissue, paracancerous tissue, chronic gastritis tissue, normal gastric mucosa tissue and other different tissues (p> 0.05). (8) There were significant differences in the expression of VEGF protein in 4 different tissues, such as gastric cancer, para cancer, chronic gastritis, and normal gastric mucosa (p< 0.05). The comparison between the expression of the protein in gastric cancer tissues and other several tissues found that there were significant differences in the expression of VEGF between gastric cancer tissues and adjacent tissues, chronic gastritis tissues and normal gastric mucosa tissues (p< 0.05). (9) There were significant differences in the expression of VEGF protein in 4 different tissues, such as gastric cancer, para cancer, chronic gastritis, and normal gastric mucosa (p< 0.05). The comparison between the expression of the protein in gastric cancer tissues and other several tissues found that there were significant differences in the expression of VEGF between gastric cancer tissues and chronic gastritis tissues and normal gastric mucosa tissues(p< 0.05), but there was no difference with paracancerous tissues (p>0.05).Conclusion: (1) The expression of ELTD1 in gastric cancer was not related to microvessel density, the degree of hypoxia, the proliferation, the occurrence and development of gastric cancer. This study failed to demonstrate that ELTD1 expression is regulated by VEGF and DLL4. The expression and role of ELTD1 in gastric cancer remains to be further studied. (2) The expression of both DLL4 and VEGF proteins were related to the occurrence and development of gastric malignancies. The expression of DLL4 and VEGF were negatively correlated with the degree of tissue hypoxia and positively correlated with the microvessel density of gastric cancer. The results showed that VEGF and DLL4 are important regulators in the development of gastric cancer.

URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/201078
Collection第二临床医学院
Recommended Citation
GB/T 7714
魏友芬. ELTD1、VEGF、DLL4在胃腺癌中的表达及 临床意义[D]. 兰州. 兰州大学,2018.
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