第一临床医学院知识库

目的：评价辅酶Q10对大鼠丙泊酚输注综合征的预防作用。 

方法：清洁级健康雄性SD大鼠30只，8~10周龄，体重250~280 g。采用随机数字表法分为3组（n=10）：对照组（C组）静脉输注生理盐水；丙泊酚组（P组）静脉输注1%丙泊酚中/长链脂肪乳注射液；辅酶Q10组（CoQ10组）灌胃100 mg/kg辅酶Q10，1 h后静脉输注丙泊酚，3组输注速率前6 h为20 mg·kg^-1·h^-1，后6 h为40 mg·kg^-1·h^-1，总时间为12 h。分别于给药即刻（T₀）、6 h（T₁）和12 h（T₂）时采集颈总动脉血样2 ml，其中0.5 ml行血气分析，1.5 ml检测血清AST、ALT、CK、CK-MB、cTnI、BUN、Cr水平。采血完毕后处死大鼠，取心肌组织观察病理学结果。

结果：与C组比较，P组和CoQ10组T_1，2时血清AST、ALT、CK、CK-MB和cTnI水平升高（P<0.05），BUN、Cr水平差异无统计学意义（P>0.05），心肌病理学损伤加重；与P组比较，CoQ10组T_1，2时血清AST、ALT、CK、CK-MB和cTnI水平降低（P<0.05），BUN、Cr水平差异无统计学意义（P>0.05），心肌病理学损伤减轻。

结论：辅酶Q10可有效预防大鼠丙泊酚输注综合征的发生。

Objective：To evaluate the efficacy of coenzyme Q10 in preventing propofol infusion syndrome in rats.

Methods：Thirty pathogen-free male Sprague-Dawley rats, aged 8－10 weeks, weighing 250－280 g, were randomly divided into 3 groups (n=10 each) using a random number table: control group (group C), propofol group (group P) and coenzyme Q10 group (group CoQ10). Normal saline was infused intravenously in group C. In group P, 1% propofol in medium- and long-chain triglyceride emulsion injection was infused intravenously. In group CoQ10, CoQ10 100 mg/kg was administrated by intragastric gavage, and 1 h later propofol was infused intravenously. The infusion rate was 20 mg·kg^-1·h^-1 within the first 6 h and 40 mg·kg^-1·h^-1 for the rest 6 h, and the total time was 12 h in the three groups. Immediately after the start of administration (T₀), and at 6 and 12 h after the start of administration(T_1,2), blood samples 2 ml were taken from the common carotid artery, with 0.5 ml for blood gas analysis and 1.5 ml for determination of the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), creatine kinase isoenzyme-MB (CK-MB), cardiac troponin I (cTnI), blood urea nitrogen (BUN）and creatinine (Cr). After blood sampling, the rats were sacrificed, and myocardial tissues were obtained for pathological examination.

Results：Compared with group C, the serum AST, ALT, CK, CK-MB and cTnI levels were significantly increased at T_1,2 (P<0.05), no significant changes were found in serum BUN and Cr levels (P>0.05), the pathological changes of myocardium were aggravated in P and CoQ10 groups. Compared with group P, the serum AST, ALT, CK, CK-MB and cTnI levels were significantly decreased at T_1,2 (P<0.05), no significant changes were found in serum BUN and Cr levels (P>0.05), and the pathological changes of myocardium were attenuated in group CoQ10. 

Conclusion：Coenzyme Q10 can effectively prevent the development of propofol infusion syndrome in rats.