| 结直肠癌组织中EpCAMhigh/CD44+癌干细胞的分布、数量及 |
| Alternative Title | A PRELIMINARY STUDY OF EpCAMhigh/CD44+CANCER STEM CELLS
|
| 张秋菊 |
| Subtype | 硕士
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| Thesis Advisor | 刘斌
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| 2009-05-25
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 结直肠癌
肿瘤干细胞
ESA
EpCAM
CD326
CD44
CD133
免疫组织化学染色
免疫组织化学双重染色
|
| Abstract | 目的
探讨原发性结直肠癌组织中ESA和CD44蛋白表达的关系及其临床病理意义，从而进一步检测EpCAMhigh/CD44+的结直肠癌干细胞在癌组织原位中的分布、数量、形态特征，并探讨其与肿瘤发生发展机制及预后的关系，并比较结直肠癌组织中CD133和CD44两者作为结直肠癌干细胞标记的价值。
方法
采用免疫组织化学SP法检测10例正常肠黏膜、13例伴不典型增生的结直肠腺瘤和67例癌组织中ESA 、CD44及CD133表达与结直肠癌干细胞的关系；采石蜡连续切片、常规HE、免疫组化及双重免疫组化方法定位癌组织中的结直肠癌干细胞，光镜下观察其数量、分布及形态特征。
结果
1．χ2检验显示结直肠癌组织中ESA和CD44的表达明显高于其在正常肠粘膜组织和腺瘤中的表达；在结直肠癌中，CD44的表达与淋巴结转移，分化程度及Dukes分期有关（P<0.05）；ESA的表达与年龄、浸润深度、分化程度及Dukes分期有关（P<0.05）。
2．Spearman等级相关分析显示ESA的表达与CD44阳性细胞的数量呈正相关（rs =0.614 P=0.000）。
3．结直肠癌中，ESA和CD44双阳性细胞的数量约占肿瘤细胞的0.1%～30%，它们沿腺管样结构的基底侧呈局灶状或散在分布；细胞呈卵圆形或立方状，胞浆稀少，胞核相对规则，均质深染，呈卵圆形或高柱状；且细胞数量与结直肠癌的分化程度呈负相关，分化越低，数量越多。
4．CD133在癌组织中阳性细胞呈片状分布，CD133的表达与各病理参数均无相关性（P＞0.05）；CD44(+)细胞散在分布，CD44的表达与淋巴结转移，分化程度及Dukes分期有关（P＜0.05），但两者在癌组织中的表达无关（P＞0.05）；CD133(+)细胞的数量明显多于CD44(+)的细胞，且CD44(+)的细胞CD133均呈阳性表达，而CD133(+)的细胞CD44部分阳性。
结论
1．ESA与CD44在结直肠癌的发生发展、浸润转移中起重要作用且密切相关，两者可能形成复合体，协同作用，共同表达在结直肠癌干细胞上；同时也作为结直肠癌干细胞的表面标记；同步检测它们在结直肠癌组织中的表达并综合分析两者之间的关系对评价结直肠癌的侵袭转移能力及预后具有一定价值。
2．结直肠癌中，双阳性细胞的数量约占肿瘤细胞的0.1%～30%，它们沿腺管样结构的基底侧呈局灶状或散在分布；细胞呈卵圆形或立方状，胞浆稀少，胞核相对规则，均质深染，呈卵圆形或高柱状；且细胞数量与结直肠癌的分化程度呈负相关，分化越低，数量越多。
3．CD133和CD44同时作为肿瘤干细胞候选的表面标记，在结直肠癌组织中均有不同程度表达，本实验从形态和功能方面证实，CD44作为结直肠癌干细胞的特异性标记比CD133更有价值。 |
| Other Abstract | Purpose
To investigate the relationship between the expressions of ESA and CD44 and their clinic- opathologic significance in primary colorectal carcinoma, furtherly to examine the distribution, quantity and morphological characteristics of the EpCAMhigh/CD44+ colorectal cancer stem cells (Co-CSCs)in situ tissue, and explore the relationship between cancer stem cells (CSCs) and tumorigenesis mechanism and prognosis. The value of CD133 and CD44 as cancer stem cells markers were comparised.
Methods
The expressions of CD44, ESA and CD133 protein in 10 cases of normal mucosa,13 cases of adenoma with atypical hyperplasia and 67 cases of colorectal carcinoma were detected by SP immunohistochemical method, and the relationship between CD44 and ESA with different pathological parameters were studied. The locations of duble-ESA and CD44 positive cells were detected by HE staining, SP immunohistochemical method and double immunohistochemical staining on continous slide.
Results
1. The expressions of CD44 and ESA in colorectal carcinoma(100%, 53.7%) were significantly higher than in normal mucosa(00.0%, 00.0%) and adenoma with atypical hyperplasia(7.1%, 00.0%). Significant relationship was found among the expression of CD44 and lymphatica metastasis (χ2=8.375 P=0.015), pathological grade (χ2=14.284 P=0.006), and Dukes stage（χ2=7.948 P=0.019）. The expression of ESA was correlated with age (χ2=16.918 P=0.000), depth of infiltration（χ2=15.195 P=0.001）, pathological grade （χ2=10.395 P=0.034）and Dukes stage（χ2=7.681 P=0.021）.
2. The expressions of ESA and CD44 were positive correlation（rs=0.614 P=0.000）
3. The number of double-positive ESA and CD44 cells accounted for 0.1% to 30% in all tumor cells; they was scattered or focal distribution along the basement of glandular-like structure; The cells were cube or oval, the cytoplasm was scarce, and the nucleus were oval or cylindrical high, relatively rules, deep stained and homogeneous; The number of double-positive cells and colorectal cancer was negatively correlated with the degree of differentiation.
Conclusion
1. The expressions ESA and CD44 are highly related to occurrence, development, infiltration and metastasis of colorectal carcinoma, they may form a complexes co-expressing on colorectal cancer stem cells. ESA and CD44 are very applicable the surface markers of colorectal cancer stem cells for the diagnosis of carcinoma metastasis and evaluating the prognosis of the patients... |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/215924
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| Collection | 基础医学院
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Recommended Citation
GB/T 7714 |
张秋菊. 结直肠癌组织中EpCAMhigh/CD44+癌干细胞的分布、数量及[D]. 兰州. 兰州大学,2009.
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