| 结直肠癌中CD44+/CD166+癌干细胞的特征及临床病理学意义 |
| Alternative Title | The Feature and Clinic Pathological Significance of CD44+/CD166+ Cancer Stem Cells in Colorectal Carcinoma
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| 罗雁 |
| Subtype | 硕士
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| Thesis Advisor | 刘斌
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| 2011-05-26
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 结直肠癌
肿瘤干细胞
CD44
CD166
组织芯片
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| Abstract | 目的：观察结直肠癌干细胞CD44+/CD166+在正常肠粘膜、腺瘤及结直肠癌组织中及人结直肠癌细胞系(SW480)中的表达及形态特征。
方法:取61例结直肠癌、10例正常肠粘膜组织及18例伴不典型增生的结直肠腺瘤制成42点的共三块组织芯片，应用免疫组织化学及免疫组织化学双染法，定位标记CD44和CD166双阳性细胞。采用流式细胞仪分析SW480中连续三代细胞所处的周期，观察阳性细胞的形态及表达。
结果:
1. 结直肠癌中，CD44的表达与病理分级及浸润深度有关（χ2 = 19.882，6.459，P<0.05），CD166的表达与病理分级及浸润深度有关（χ2= 10.802，8.041，P<0.05），CD44与CD166的表达呈正相关（r=0.333, P=0.009<0.01）。
2. 结直肠癌中双阳性细胞沿腺管的基底侧散在分布。分化程度的降低，数量增多（r=0.449 P=0.000<0.01），浸润深度的加深，细胞的数量增多（r=0.291 P=0.023<0.05）。
3. 人结直肠癌细胞系SW480中的小圆形细胞，CD44和CD166呈胞浆黄染。与结直肠癌实体瘤中的圆形细胞形态相似，可能为肿瘤干细胞。
结论:
1.CD44与CD166作为结直肠癌干细胞的表面标记物与结直肠癌的分化程度及浸润程度密切相关。
2.观察结直肠癌干细胞的数量、分布、HE染色，为结直肠癌的病理诊断和分期提供参考。
3.SW480中的小圆形细胞与结直肠癌实体瘤中的圆形细胞的形态和CD44与CD166免疫标记特征相同，具备肿瘤干细胞的特征。 |
| Other Abstract | Objective:To investigate the expressions and the morphologic features of the cancer stem cell
CD44+/CD166+ in normal mucosa, adenoma and adenocarcinoma and SW480.
Methods: 61 cases of colorectal carcinoma, 10 cases of normal mucosa and 18 cases of adenoma were collected and made into three tissue microarrays each containing 42 dots. The locations of CD44+/CD166+ were detected by immunohistochemical staining and double immunohistochemical staining. The cell cycle of the three successive of SW480 was analyzed by Flow Cytometry. Investigate the expressions and morphologic features of double positive cells.
Results:
1. Significant relationship was found among the expression of CD44, pathological grade and depth of invasion（χ2 = 19.882，6.459，P<0.05）. The expression of CD166 was also correlated with pathological grade and depth of invasion（χ2 = 10.802，8.041，P<0.05）. There was a positive correlation between the expressions of CD44 and CD166（r=0.333, P=0.009<0.01）.
2.The quantity of double-positive cells were positively correlated with the differentiation and the depth of invasion of colorectal carcinoma（r=0.449 P<0.01;r=0.291 P<0.05）.
3. The small-round cells were similar with the round cells of the colorectal carcinoma. Strong cytoplasm CD44、CD166 staining in SW480 .
Conclusion:
1. As the surface markers of colorectal cancer stem cell, CD44 and CD166 are highly related to differentiation and invasion of colorectal carcinoma.
2. To observe the number, distribution and morphology of CSC can contribute to the diagnosis and prognostic evaluation of colorectal carcinoma.
3. The morphology and immunophenotype of small-round cells in SW480 are similar with the small round cells in colorectal carcinoma . The small-round cells have the characteristics of cancer stem cells. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/215928
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| Collection | 基础医学院
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Recommended Citation
GB/T 7714 |
罗雁. 结直肠癌中CD44+/CD166+癌干细胞的特征及临床病理学意义[D]. 兰州. 兰州大学,2011.
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