| 结直肠癌中CD44+/ALDH1+癌干细胞的临床病理学意义 |
| Alternative Title | The significance of clinical pathology of CD44+/ALDH1+ cancer stem cells in colorectal carcinomas
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| 张彩兰 |
| Subtype | 硕士
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| Thesis Advisor | 刘斌
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| 2013-05-23
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 肿瘤干细胞
结直肠癌干细胞
CD44+/ALDH1+
SW480
组织芯片
免疫组织化学染色
免疫组织化学双重染色
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| Abstract | 目的 研究肿瘤干细胞标志物CD44和ALDH1在人结直肠癌组织中的表达、相互关系及临床病理学意义；探讨并进一步比较CD44+/ALDH1+细胞在人结直肠癌、伴不典型增生的管状腺瘤、正常肠粘膜组织中的数量、位置及形态特征；进一步研究人结肠癌细胞系SW480中CD44+/ALDH1+细胞的表达数量及形态特征，来论证CD44+/ALDH1+细胞作为结直肠癌干细胞标志物的价值。方法 取68例人结直肠癌组织、20例伴不典型增生的管状腺瘤、12例正常肠粘膜组织，利用组织芯片仪制作组织芯片，制成(6×7列)组织芯片共三块；采用HE染色、免疫组织化学染色、免疫组织化学双重染色技术，定位标记CD44、ALDH1单阳性及双阳性细胞；SW480结肠癌细胞系，细胞培养后制备细胞爬片，采用HE染色、免疫组织化学染色、免疫组织化学双重染色技术进一步观察双阳性细胞的数量、形态特征。 |
| Other Abstract | Objective To investigate the expression, relationship and its clinicopathologic significance of the cancer stem cells(CSCs) makers CD44 and ALDH1 in colorectal carcinomas. Further to compare the quantity,location, and morphological characteistics of colorectal cacer stem cells CD44+/ALDH1+
in tubular adenoma, colorectal carcinomas and normal intestinal mucosa.To observe the morphological characteristics, quantity of CD44+/ALDH1+ cells in human colon cancer cell lines(SW480). Further more to demonstrate the value of CD44+/ALDH1+ as a marker of colorectal cancer stem cells(Co-CSCs).Methods 68 cases colorectal cancer, 20 cases tubular adenoma and 12 cases normal intestinal mucosa were collected and made into three tissue microarray(6×7) by microarray instrument. The expression of CD44 and ALDH1were detected by HE staining, immunohistochemical staining and double immunohistochemical staining technology. The location, morphological characteristics, quantity of CD44+/ALDH1+ cells in human colon cancer cell lines(SW480) were detected by HE staining, immunohistochemical staining and double immunohistochemical staining technology. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/215929
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| Collection | 基础医学院
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Recommended Citation
GB/T 7714 |
张彩兰. 结直肠癌中CD44+/ALDH1+癌干细胞的临床病理学意义[D]. 兰州. 兰州大学,2013.
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