兰州大学机构库 >生命科学学院
六种不同盐爪爪属(Kalidium)植物的抗盐差异性分析
Alternative TitleAnalysis of different salt tolerance of six species in genus Kalidium
刘得成
Thesis Advisor吴雨霞
2018-04-13
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword盐爪爪属(Kalidium) 盐生植物 总含盐量 离子含量 pH 萌发率 脯氨酸
Abstract

耐盐性是一种复杂的性状,被认为在植物中是多次起源的。在进化过程中,各种盐生植物的重新适应,使得其能生活于高盐度的环境中。盐爪爪属(Kalidium)植物,作为一种肉质化的稀盐型真盐盐生植物,共有六种,包括尖叶盐爪爪(Kalidium. cuspidatum),盐爪爪(Kalidium. foliatum),细枝盐爪爪(Kalidium. gracile),黄毛头(Kalidium. sinicum),里海盐爪爪(Kalidium. caspicum)及圆叶盐爪爪(Kalidium. schrenkianum),在中国主要分布在荒漠与半荒漠的盐碱地区。本研究在野外充分调查的基础上,分别从六种不同盐爪爪属植物分布的地区采集土样 (0-100 cm),对土壤pH值及总含盐量进行分析,并测定土壤样品中Na,K+,Ca2+,Mg2+,CO32-,Cl-,HCO3-,SO42-含量。同时用不同浓度的NaCl(0-500 mM)和Na2SO4 (0-250 mM)对野外收集的盐爪爪属种子的萌发及幼苗根的生长进行测定。此外,用不同浓度的NaCl处理六个种的四个月幼龄的植株,一个月后测定相应的生理指标。研究结果发现: 1)盐爪爪属植物所分布土壤环境中的pH值范围主要集中在8.5-10.5;尖叶盐爪爪(K. cuspidatum),细枝盐爪爪(K. gracile),黄毛头(K. sinicum),盐爪爪(K. foliatum)生长土壤里总含盐量依次为72.37 g/kg,74.69 g/kg,76.50 g/kg,66.46 g/kg,而里海盐爪爪(K. caspicum)和圆叶盐爪爪(K. schrenkianum)分别为51.48 g/kg和12.98 g/kg。在所有土壤样品,Na是最丰富的阳离子,并与所有深度下(20-40 cm除外)总含盐量呈极显著的正相关关系。尖叶盐爪爪(K. cuspidatum)分布的地区Cl-有较高含量为10.62 g/kg,而里海盐爪爪(K. caspicum)分布的地区SO42-含量可高达11.78 g/kg; 2)六个种种子的萌发率均与NaCl及Na2SO4的浓度呈负相关。在200 mM NaCl处理下,尖叶盐爪爪(K. cuspidatum)种子的萌发率仍能达到45 %;而在150 mM Na2SO4处理中,里海盐爪爪(K. caspicum)种子萌发率最大值可达55 %。尖叶盐爪爪(K. cuspidatum)根的生长量在300 mM NaCl处理下最高为49.93 %;而当Na2SO4浓度为150 mM时,里海盐爪爪(K. caspicum)的最大值仅次于尖叶盐爪爪(K. cuspidatum)及盐爪爪(K. foliatum)为21.23 %; 3)300 mM NaCl下,黄毛头(K. sinicum)的生长受到抑制,株高降低了22.57 %;含水量最低仅为47.73 %;三种抗氧化酶(SOD、CAT、POD)的活性均下降;而脯氨酸的含量最高为361.62 μg/g FW。400 mM NaCl下,里海盐爪爪(K. caspicum)内的SOD,CAT,POD三种酶的活性均有最大值分别为144.49 U/g FW,163.66 nmol/min/ g FW,14933.74 U/g FW。500 mM NaCl 处理时,尖叶盐爪爪(K. cuspidatum),盐爪爪(K. foliatum)及细枝盐爪爪(K. gracile)仍能生存,各个种内的脯氨酸含量均达到最大值,分别为412.17 μg/g FW,248.77 μg/g FW,172.98 μg/g FW。此时盐爪爪(K. foliatum)及细枝盐爪爪(K. gracile)的SOD和CAT活性也最大。尖叶盐爪爪(K. cuspidatum)的叶绿素含量最高为0.26 mg/g FW。盐胁迫处理后,土壤总含盐量,盐爪爪属六个种内总体的Na+水平,脯氨酸及丙二醛的含量与盐浓度呈正相关关系。而甜菜碱的含量变化并不显著。 结合以上结果可发现:尖叶盐爪爪(K. cuspidatum)和里海盐爪爪(K. caspicum)所在生境中分别以NaCl和Na2SO4为主;尖叶盐爪爪(K. cuspidatum)、盐爪爪(K. foliatum)及细枝盐爪爪(K. gracile)对盐具有较强的耐受性,而里海盐爪爪(K. caspicum),圆叶盐爪爪(K. schrenkianum)则相对较弱,黄毛头(K. sinicum)的耐盐性最低。不同地区土壤中的含盐量,pH值及不同离子的浓度,对盐爪爪属植物的谱系地理分布具有明显的选择作用;盐爪爪属六个种不同的抗盐差异性主要是由其生境中的盐分所决定的。

Other Abstract

Salt tolerance is a complex trait that is thought to have multiple origins in plants, and during the evolution, various halophytes readapt to make them live in high salinity environment. Kalidium (Chenopodiaceae) is a genus of succulent euhalophytes with six species has a certain ability to adapt to saline-alkali soil and desert areas in Northwest China. On the basis of full field survey, soil samples (0-100 cm) from the different areas where six species of Kalidium distributed were collected. pH value, total salt contents, and the concentrations of Na, K, Ca2+, Mg2+, CO32-, Cl-, HCO3-, SO42- from the soil samples were analyzed. Seeds germination and the growth of roots among six species were tested under different concentrations of NaCl (0-500 mM) and Na2SO4 (0-250 mM). Then the germination percentage and recovery germination percentage, and the growth percentage of roots under different concentrations were calculated. The relative physiological indexes of four months old seedlings were determined after treatments with different contents of NaCl (300 mM,400 mM,500mM) for 30 days. The results are as following: 1) pH value mainly ranges from 8.5-10.5;The total salt concentrations of the soil where K. cuspidatum, K. gracile, K. sinicum, K. foliatum located can be as high as 72.37 g/kg, 74.69 g/kg, 76.50 g/kg, 66.46 g/kg, and the maximum of K. caspicum and K. schrenkianum only are 51.48 g/kg, 12.98 g/kg, respectively. Nais the most abundant cation in all soil samples, and has a very significant positive correlation with total salt contents at all depths (except 20-40 cm). Cl- with a higher concentration of 10.62 g/kg in K. cuspidatum distributed area, and the content of SO42- is as high as 11.78 g/kg in K. capsicum distributed areas; 2) The germination percentages of six species are negatively correlated with the increase concentration of NaCl and Na2SO4, the germination percentage still can reach 45% in K. cuspidatum at a higher concentration of NaCl (200 mM). While in Na2SO4, the maximum of germination percentage is 55 % (150 mM) in K. capsicum, the growth percentage of radicle in K. cuspidatum is the highest as 49.93 % in 300 mM NaCl; and in 150 mM Na2SO4, the maximum of K. capsicum is 21.23 % followed after K. cuspidatum and K. foliatum; 3) After treatment with 300 mM NaCl, the growth of K. sinicum is inhibited, and the plant height is decreased by 22.57 % than the control; water content is only about 47.73 % which below 50 %; and the activities of three antioxidant enzymes (SOD, CAT, POD) are also decreased; While the proline content was also highest as 361.62 μg/g FW. When the concentration is high as 400 mM, the activities of SOD, CAT and POD in the K. capsicum are 144.49 U/g FW, 163.66 nmol/min/ g FW, 14933.74 U/g FW, respectively. Under the extreme concentration (500 mM), However, K. cuspidatum, K. foliatum, K. gracile are still able to survive, and the proline contents in each species reached the maximum of 412.17 μg/g FW, 248.77 μg/g FW, 172.98 μg/g FW, respectively. The activities of SOD and CAT in K. foliatum、K. gracile can also be the highest. While in K. cuspidatum the total chlorophyll content is 0.26 mg/g FW, which is higher than others. In summy, the total salt contents of soil, Na+ level in the six Kalidium species is positively related to the increasing concentration of NaCl on the whole, and so as well the proline and malondialdehyde. However, there is no significant difference in glycine betaine. These findings suggest that adaptation to topsoil salinity in early stages is a major determinant of the six species’ geographical distributions. However, relative proportions of the ions are also apparently important as K. cuspidatum and K. caspicum occupy areas where the major anions are Cl- and SO42-, respectively. The differences in salt content, pH value and ion concentration of different areas can make one obvious choice to the spectrum distribution of Kalidium; and the different salt tolerance of six species in genus Kalidium are mainly determined by the salt in their habitat.

URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/220858
Collection生命科学学院
Recommended Citation
GB/T 7714
刘得成. 六种不同盐爪爪属(Kalidium)植物的抗盐差异性分析[D]. 兰州. 兰州大学,2018.
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