核孔蛋白及R蛋白参与BAK1 BKK1介导的细胞死亡调控机理的研究

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	核孔蛋白及R蛋白参与BAK1 BKK1介导的细胞死亡调控机理的研究
Alternative Title	Several Nucleoporins and R Proteins Are Essential for BAK1 and BKK1-Mediated Cell-Death Control in Arabidopsis thaliana
	詹延延
Thesis Advisor	黎家
	2015-06-03
Degree Grantor	兰州大学
Place of Conferral	兰州
Degree Name	硕士
Keyword	细胞死亡 BAK1 BKK1 核孔蛋白 R蛋白
Abstract	在拟南芥中BAK1及其同源物BKK1能参与不依赖油菜素内酯信号转导的自发性细胞死亡调控，这种死亡与水杨酸的积累密切相关。bak1-4 bkk1-1强双突变体在无菌条件下萌发一周后就会发生致死性的细胞死亡。目前关于这种细胞死亡信号转导的具体分子机理还有待研究。bak1-3 bkk1-1由于仍有部分野生型BAK1 mRNA的表达，它的死亡表型明显弱于bak1-4 bkk1-1。因此我们以bak1-3 bkk1-1为背景，遗传筛选得到了一个遗传抑制子sbb1-1，它能很好地抑制bak1-3 bkk1-1的死亡表型。遗传分析显示这种死亡受抑制的表型是由SBB1的缺失突变造成的，克隆发现SBB1编码NUP107-160核孔亚复合体中的一个亚基蛋白Nup85。运用反向遗传学方法，我们首先证明了SBB1选择性地调控BAK1 BKK1介导的细胞死亡，bir1、bon1和mekk1诱发的细胞死亡不依赖SBB1的功能。其次，我们的遗传证据还证明了部分Nup107-160亚复合体成员及SBB1相关蛋白RNA解旋酶DRH1也是拟南芥中BAK1 BKK1介导的细胞死亡信号通路上的重要蛋白。最后，我们通过RNAi方法证明R蛋白RBB1家族同样调控BAK1 BKK1介导的细胞死亡。
Other Abstract	Arabidopsis BAK1 and its paralog BKK1 were found to regulate BR-independent and salicylic acid (SA)-dependent spontaneous cell-death pathways. A double null mutant bak1-4 bkk1-1 displays a lethality phenotype about one week after germination even when grown under sterile conditions .To date, molecular mechanisms of the cell-death signal transduction remain unclear. bak1-3 bkk1-1 double mutant,however,can express a reduced amount of wild-type BAK1 mRNA and shows a much reduced cell-death phenotype than bak1-4 bkk1-1. A genetic suppressor named sbb1-1 was identified via genetic modifier screen for the weak mutant. Genetic analyses show that the cell-death suppression was caused by a loss-of-function mutant of SBB1 which encodes a nucleoporin (NUP) 85-like protein belonging to the NUP107-160 subcomplex. Here we report that SBB1 is a novel regulator fundamental for BAK1 and BKK1-mediated cell-death signaling pathway, but not required for bir1，bon1 or mekk1-triggered cell death control. Our genetic evidence also demonstrated that members of NUP107-160 subcomplex and SBB1 associated DRH1 are essential for BAK1 and BKK1-mediated spontaneous cell death signaling pathway in Arabidopsis. Further, RBB1 family genes were identified playing important roles in modulating bak1 bkk1-conferred cell death control by using an RNAi approach.
URL	查看原文
Language	中文
Document Type	学位论文
Identifier	https://ir.lzu.edu.cn/handle/262010/221250
Collection	生命科学学院
Recommended Citation GB/T 7714	詹延延. 核孔蛋白及R蛋白参与BAK1 BKK1介导的细胞死亡调控机理的研究[D]. 兰州. 兰州大学,2015.

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