|Alternative Title||Reproductive toxicity of inhalable particulate matter containing cadmium to male Kunming mice in different ages|
|Place of Conferral||兰州|
|Keyword||雄性小鼠 镉 呼吸暴露 睾丸 附睾 生殖机能|
为了研究含镉可吸入颗粒物呼吸暴露对胚后发育不同阶段生殖系统的影响,本实验采用不同周龄的5组健康雄性昆明小鼠，分别为3周龄、4周龄、5周龄、6周龄和7周龄，囊括未性成熟、将近性成熟和完全性成熟的小鼠，每一周龄小鼠又分为处理组与对照组，每组10只。处理组用3 mM氯化镉通过气动雾化法对小鼠进行呼吸暴露染毒，每天2 h，连续染毒7天，对照组不作处理。染毒7天后两组均麻醉，取睾丸和附睾，测定小鼠睾丸和附睾的脏器系数。小鼠一侧睾丸与附睾用10%中性甲醛固定，制作并观察组织切片；另一侧负80 ℃冰冻，留作重金属检测和抗氧化能力的检测，6、7周龄小鼠该侧睾丸取出后进行精子活力检测后再冰冻保存用于组织镉含量检测。
Cadmium is a widely used heavy metal in industry, but it is non-essential element for organism. Excessive cadmium can cause serious damage to organisms. With the development of modern industry, cadmium pollution has become more and more serious, major cadmium pollution incidents have occurred frequently, and cadmium pollution has attracted more and more attention. In the past, studies on the toxicity of cadmium have focused on the liver and kidneys and so on, and there have been few studies on the reproductive system. The reproduction system is closely related to the population multiply. The toxic effects of cadmium on the liver and kidneys mainly affect individual health status, but the toxic effects of cadmium on the reproductive system will further affect the population multiply. Therefore, it is significant to explore the toxicity of cadmium on the reproductive system.
To explore the effect of respiratory exposure with inhalable particulate containing cadmium on the reproductive system at different stages of postembryonic development. Five groups of healthy male Kunming mice of different ages were used in this experiment. They were 3 weeks old, 4 weeks old, 5 weeks old, 6 weeks old and 7 weeks old. They included immature, near mature and complete mature. Rats, one week old mice were divided into treatment and control groups, with 10 mice in each group. In the treatment group, the mice were treated with 3 mM cadmium chloride (CdCl2) by pneumatic atomization. The mice were continuously exposed to CdCl2 for 2 hours per day for 7 days, and the control group was not treated. After exposure for 7 days, mice in all the groups were anesthetized. The testes and epididymis were excised to detect the organ coefficients of the mice. The unilateral testis and epididymis were fixed in 10% neutral formaldehyde, and the tissue sections were made and observed; the residual testis and epididymis were frozen at -80° C for detections on heavy metal and anti-oxidative ability in tissues. The sperm motility was measured after the testicles were removed from 6 and 7-week-old mice, and then frozen for detections heavy metal in tissues.
Research shows, cadmium has a significant effect on testicular development in young mice (3 and 4 weeks old) (P<0.05), but the effect of cadmium on the testicular development of mice after 5 weeks old is not obvious. Cadmium also caused epididymis atrophy in all age groups (P<0.05). Cadmium disturbs the arrangement of spermatogenic cells in the testicular seminiferous tubules, and some spermatogenic cells are released to the center of seminiferous tubules. Cadmium leads to narrowing of the epididymal duct and thickening of the wall of the epididymal duct. In short, cadmium treatment affects the histological structure of testis and epididymis. Cadmium also reduces sperm motility (P<0.05) and increases sperm abnormality rate (P<0.05). Cadmium treatment did not have a significant effect on MDA content and SOD activity in mice testis. However, cadmium significantly reduce SOD activity and MDA content in the epididymis of 6-week-old mice (P<0.05). The cause of this phenomenon may be the epididymis will swell rapidly during this period, cell division and proliferation are exuberant and leading to higher MDA background value, and cadmium will affect this normal state.
|郑鹏飞. 含镉可吸入颗粒物对不同周龄雄性昆明小鼠的生殖毒性[D]. 兰州. 兰州大学,2018.|
|Files in This Item:||There are no files associated with this item.|
|Recommend this item|
|Export to Endnote|
|Similar articles in Google Scholar|
|Similar articles in Baidu academic|
|Similar articles in Bing Scholar|