兰州大学机构库 >生命科学学院
Alternative TitleStudy on the Assembly Behavior of Photo-Responsive DNA Amphiphile at the LC-Aqueous Interface
Thesis Advisor王锐
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
KeywordDNA 双亲分子 液晶 组装 调控




Other Abstract

Liquid crystals (LCs) are a new class of materials state that can exhibit characteristics between the solid and the liquid state. LCs have quickly become widespread in our daily life since 1888 and are well known for their applications in electronic display technology. In addition, in the past two decades, LCs have been investigated as excellent sensing materials, with the intrinsic properties of short-range molecule-molecule interactions and long-range orientational communication that can be used to amplify and transform micro- and nanoscale molecular interaction into macroscopic measurable optical signals. Hence, they provide a label-free, low-cost, low power consumption and real-time detection platform.

Previous studies of the molecular interactions using LCs-based sensing platforms mainly focused on traditional amphiphiles, nucleic acid, proteins, bacteria and viruses. However, these kinds of functional interface have low specificity and selectivity. It is worth noting that for DNA amphiphiles, the DNA can be programmed to control its sequence and length, to be further modified by functional groups. So it is expected to improve the responsiveness and selectivity of the LC-based detection system. On the other hand, the regulation of the LC interface with the amphiphiles assembly often relies on the introduction of additional chemical substances. On the contrary, using remote and clear light stimulus to control the LC interface will provide a potential expanded scope of application, but relevant reports are few.

Therefore, the research focus of this article was to design a type of photo-responsive azobenzene-containing DNA amphiphiles (named Azo-DNA), and studied its assembly behavior at planar and cambered LC-aqueous interfaces. Additionally, the use of light to regulate the assembly behavior of the DNA amphiphiles was investigated. The preliminary experiment results showed that for a series of concentrations, Azo-DNA did not cause any optical signal change at the planar LC-aqueous interface. This differed from the traditional amphiphiles: as the assemblies of Azo-DNA are too stable to disassemble, so it is difficult for them to interact with LCs. Then we tried to mix the Azo-DNA with DLPC to promote its disassembly, for which some special optical textures were obtained that differed from the individual ones. Subsequently, we used dual- fluorescent labelling to confirm the detailed assembly behavior in suit. For the cambered interface (LC droplets), strong external ultrasonic waves effectively caused disruption of the Azo-DNA assemblies, then promoted the assembly at the LC droplets surfaces, which were cambered and with high surface energy. We successfully achieved the assembly of Azo-DNA at the LC droplets interface as well as reversibly controlling the assembly/disassembly of the LC droplets, which based on the different polar properties for trans and cis azobenzene. Currently, two reversible cycles have been achieved, laying the foundation for future work to extend this research further.

Document Type学位论文
Recommended Citation
GB/T 7714
安小苹. 光响应DNA双亲分子在液晶-水相界面的组装行为研究[D]. 兰州. 兰州大学,2017.
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