兰州大学机构库 >生命科学学院
复方四君子汤及党参多糖调节溃疡性结肠炎小鼠肠道菌群的作用研究
Alternative TitleSijunzi decoction and Codonopsis pilosula polysaccharide alleviate DSS-induced mice
陈新君
Thesis Advisor张春江
2016-05-28
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword肠道菌群 溃疡性结肠炎 益生菌 益生元 四君子汤
Abstract本研究将四种药物:四君子汤,党参多糖,布拉氏酵母菌及多糖+酵母组合分别作用于DSS诱导的小鼠急性UC模型,通过对炎症小鼠体重变化,结肠长度,病理组织改善,MPO活力的检测,比较了四种不同药物缓解结肠炎症的能力;并利用二代测序技术深入剖析四种药物干预后,不同组小鼠在整体菌群结构组成上的变化及差异,同时在不同分类学水平上发掘对不同药物干预起响应的关键菌属;分析不同药物调节肠道微生态能力,主要评估党参多糖作为益生元的潜质。 四种药物均缓解了DSS造成的小鼠体重下降、结肠缩短,组织损伤等肠炎症状。同时小鼠整体菌群结构及在多种分类学水平上的组成均发生了明显差异。通过扭转因急性炎症引起的F/B值降低,抑制有害菌,促进有益菌增殖,是上述药物恢复结肠炎小鼠菌群失调的共同作用方式。此外,研究中最为明显的结果是短链脂肪酸(SCFA)产生相关菌属的显著增殖,表现为:布拉氏酵母菌选择性促进Roseburia, Anaerovorax, Lactobacillus; 四君子汤提高Butyrivibrio, Quinella 和 Anaerotruncus 的丰度; 另外,党参多糖作为益生元的证据充分,不仅具备促进常见益生菌Bifidobacterium, Lactobacillus, Arthrobacter, Akkermansia等增殖这一基本益生能力,在鉴定出的与SCFA产生相关细菌中,有7个菌属能显著被党参多糖富集包括:Anaerovorax, Roseburia, Prevotella, Dialiste, Megamonas, Faecalibacterium,Subdoligranulum。
Other AbstractIn this study, Based on pyrosequencing, the structural changes of gut microbiota were analyzed in dextran sulphate sodium (DSS)- induced acute colitis mice during treatments with four drugs: S.boulardii, SJZT, Dangshen polysaccharide, S.boulardii+ polysaccharide. All four drugs profoundly inhibited body weight loss, colon shortening and ameliorated histological damage. All drugs were showing reverting effect on DSS-induced dysbiosis of gut microbiota in mice. Benifical microbe, especially Short Chain Fatty Acid (SCFA)-producing bacteria genus were remarkable enriched. Roseburia, Anaerovorax,Lactobacillus were selectively increased by S.boulardii. Butyrivibrio, Quinella and Anaerotruncus were selectively facilitated by SJZT. Most notably, Dangshen polysaccharide selectively promoted the most identified bacteria genus, Bifidobacterium, Arthrobacter, Akkermansia, Anaerovorax, Roseburia, Prevotella, Dialister, Megamonas, Faecalibacterium, Subdoligranulum.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/221470
Collection生命科学学院
Recommended Citation
GB/T 7714
陈新君. 复方四君子汤及党参多糖调节溃疡性结肠炎小鼠肠道菌群的作用研究[D]. 兰州. 兰州大学,2016.
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