|Alternative Title||Antinociceptive and anti-inflammatory activities and mechanisms of iridoid glycosides extract of Lamiophlomis rotata (Benth.) Kudo
|Place of Conferral||兰州
2. 研究总环烯醚萜苷和8-O-乙酰山栀子苷甲酯、Phloyoside II和山栀子苷甲酯的体外抗炎活性。
1.Extract and enrich the iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo (IGLR), and study on its antinociceptive and anti-inflammatory activities in mice.
2.Study the anti-inflammatory activity of IGLR and 8-O-acetyl-shanzhiside methylester, phloyoside II and shanzhiside methylester in vitro by determining the concentrations of NO and PGE2 in LPS treated-macrophage RAW264.7.
3.Study on the compositions and the contents of iridoid glycosides between Lamiophlomis rotata and Phlomis umbrosa.
1.Combining with the macro-porous adsorptive resins and polyamide chromatographic method, IGLR were extracted. UV and HPLC were used to qualitation and quantification analyse the iridoid glycosides.
2.After IGLR were administrated to each mouse. Several classical mice model were adopted to evaluate the antinociceptive and anti-inflammatory activities
3.The concentrations of NO and PGE2 in cell were determined and used to evaluate the anti-inflammatory activity of IGLR and three compounds. MTT test was employed to evaluate the cytotoxicity .
4.TLC was used to identify iridoid glycosides; and HPLC was used to determine the content of 8-O-acetylshanzhiside methylester.
1.The ultraviolet absorption of the total iridoid glycoside was very similar to that of 8-O-acetyl shanzhiside methylester.
2.The iridoid glycosides have the significant antinociceptive and anti-inflammatory effects in vivo.
3.The iridoid glycosides and three compounds could inhibit the release of NO and PGE2, but no dose dependent was showed.
4.L. rotata and P. umbrosa have the plenty of iridoid glycosides and similar chromatograms.
1.The IGLR was enriched and extracted successfully from the aqueous extract with the macro-porous adsorptive resins and polyamide chromatographic method.
2.IGLR have the significant antinociceptive and anti-inflammatory effects in vivo.
3.IGLR and three compounds have the significant anti-inflammatory effect in vitro, and no cell toxicity.
4.The roots of L. rotata and P. umbrosa have the similar iridoid glycosides.|
尚小飞. 独一味总环烯醚萜苷镇痛抗炎作用及其机理研究[D]. 兰州. 兰州大学,2010.
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