藏药螃蟹甲的化学成分研究

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	藏药螃蟹甲的化学成分研究
Alternative Title	Studies on the chemical constituents of phlomis younghusbandii
	张超
Thesis Advisor	贾正平
	2011-05-21
Degree Grantor	兰州大学
Place of Conferral	兰州
Degree Name	硕士
Keyword	螃蟹甲环烯醚萜苷苯乙醇苷独一味糙苏萝卜秦艽 HPLC 镇痛抗炎
Abstract	目的 1. 研究藏药螃蟹甲的化学成分。 2. 建立HPLC法测定藏药螃蟹甲中环烯醚萜苷和苯乙醇苷类化合物的含量。 3. 研究比较螃蟹甲、独一味、糙苏和萝卜秦艽中的环烯醚萜苷和苯乙醇苷类成分和含量，为它们的进一步开发和利用提供借鉴。 4. 研究藏药螃蟹甲的镇痛抗炎作用。方法 1. 运用大孔吸附树脂、薄层层析色谱、硅胶柱色谱和反相制备型高效液相色谱等现代分离技术对螃蟹甲的化学成分进行提取﹑分离与纯化，得到单体化合物，然后利用理化性质和质谱﹑核磁共振等波谱技术鉴定化合物的结构。 2. 运用高效液相色谱（HPLC）法对螃蟹甲中5个环烯醚萜苷：sesamoside，shanzhiside methyl ester，7,8-dehydropenstemoside，penstemoside，8-O-acetylshan- zhiside methyl ester和3个苯乙醇苷：acteoside，alyssonoside，isoacteoside的含量进行测定。色谱柱为Symmetry C18 (4.6 mm ×150 mm，5 μm)，检测环烯醚萜苷的流动相为乙腈-水梯度洗脱，0～5 min：7% 乙腈，5～10 min：7%-12% 乙腈，10～40 min：12%乙腈，流速1.0 mL•min-1，柱温20 °C，检测波长为235 nm；检测苯乙醇苷的流动相为甲醇：水（36：64），HAc调pH至4.5，流速1.0 mL•min-1，柱温20 °C，检测波长为330 nm。 3. 通过建立HPLC法测定螃蟹甲、独一味、糙苏与萝卜秦艽中的5个环烯醚萜苷：sesamoside，shanzhiside methyl ester，7,8-dehydropenstemoside，penstemoside，8-O-acetylshanzhiside methyl ester和3个苯乙醇苷：acteoside，alyssonoside，isoacteoside的含量，来初步比较这四种药用植物中的环烯醚萜苷和苯乙醇苷类成分和含量。 4. 螃蟹甲水提取物小鼠一次腹腔注射给药后，采用小鼠扭体法、热板法、耳肿胀法、足肿胀法及毛细血管通透性实验等方法来观察其镇痛抗炎作用。结果 1. 从螃蟹甲中分离得到了14个化合物，分别为shanzhiside methyl ester (1)，sesamoside (2)，7,8-dehydropenstemoside (3)，penstemoside (4)，8-O-acetylshanzhi- side methyl ester (5)，phlomiol (6) ，acteoside (7)，alyssonoside (8)，isoacteoside (9)，三十烷酸(10)，三十烷酸对羟基苯乙酯(11)，β-谷甾醇(12)，β-谷甾醇葡萄糖苷(13)和蔗糖(14)。 2. 环烯醚萜苷类化合物sesamoside，shanzhiside methyl ester，7,8- dehydropenstemoside，penstemoside，8-O-acetylshanzhiside methyl ester和苯乙醇苷类化合物acteoside，alyssonoside，isoacteoside分别在48～648 (r=0.999 3)，48～336(r=0.999 5)，40～280(r=0.999 4)，10～70(r=0.999 6)，40～280(r=0.999 7) ，20～500(r=0.999 6)，10～130(r=0.999 5)，10～130 (r=0.999 7)μg•mL-1范围内与峰面积呈良好线性关系，并且平均加样回收率均在96%～104%之间，RSD均小于5%(n=6)。 3. 螃蟹甲、独一味、糙苏和萝卜秦艽具有相似的高效液相色谱图，并且都含有大量的环烯醚萜苷和苯乙醇苷类成分，尤其含量较大的sesamoside，shanzhiside methyl este，acteoside更是广泛分布于这四种药用植物中。 4. 螃蟹甲水提取物各剂量组能显著减少小鼠扭体反应的次数；只有高剂...
Other Abstract	Objective: 1.To study the chemical constituents of Phlomis younghusbandii. 2.To establish RP-HPLC methods for the determination of iridoid glycosides and phenylethanoid glycosides, respectively in Phlomis younghusbandii. 3.To study on the compositions and the contents of iridoid glycosides and phenylethanoid glycosides among Phlomis younghusbandii、Lamioplomis rotata、Phlomis umbrosa and Phlomis medicinalis Diels, which can provide reference for their further development. 4.To study the analgesic and anti-inflammatory effects of Phlomis younghusbandii. Methods: 1.Chemical constituents were isolated and purified by macroporous resin, TLC, silica gel column chromatography and Pre-HPLC．Their structures were identified by physical and chemical properties and MS, NMR spectra date. 2.Five iridoid glycosides (sesamoside, shanzhiside methyl ester, 7,8-dehydropenstemoside, penstemoside, 8-O-acetylshanzhiside methyl ester) and three phenylethanoid glycosides (acteoside, alyssonoside, isoacteoside) were determined by HPLC. HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm, Waters) column, for the iridoid glycosides the mobile phase was gradient elution of acetonitrile-water, 0~5 min: 7% acetonitrile; 5~10 min: 7% -12% acetonitrile; 10~40 min: 12% acetonitrile. The flow rate was 1.0 mL•min-1. The column temperature was 20 °C and the detection wavelength was 235 nm; for the phenylethanoid glycosides the mobile phase was the mixture of methanol-water (36:64, pH=4.5, HAc). The flow rate was 1.0 mL•min-1. The column temperature was 20 °C and the detection wavelength was 330 nm. 3.HPLC was used to identify and determine the content of iridoid glycosides and phenylethanoid glycosides in the four medicinal plants: Phlomis younghusbandii、Lamioplomis rotata、Phlomis umbrosa and Phlomis medicinalis Diels. 4.Administered the water extract of Phlomis younghusbandii by intraperitoneal injection, the acetic-acid induced writhing times, the hot plate threshold, carrageenan-induced hind paw edema, xylene-induced ear swelling, and acetic acid induced the peritoneal capillary permeability in mice were observed and determined to evaluate the analgesic and anti-inflammatory activities of Phlomis younghusbandii. Results: 1.Fourteen compounds were isolated and identified respectively as shanzhiside methyl ester (1), sesamoside (2), 7,8-dehydropenstemoside (3), penstemoside (4), 8-O-acetylshanzhiside methyl ester (5), phlomiol (6), acteoside (7), alyss...
URL	查看原文
Language	中文
Document Type	学位论文
Identifier	https://ir.lzu.edu.cn/handle/262010/221650
Collection	生命科学学院
Recommended Citation GB/T 7714	张超. 藏药螃蟹甲的化学成分研究[D]. 兰州. 兰州大学,2011.

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