兰州大学机构库 >生命科学学院
不同生境土壤中硝化和反硝化微生物群落结构的研究
Alternative TitleStudy on Community Structure of Nitrifiers and Denitrifiers in Soil of Different Habitats
南怀燕
Thesis Advisor马晓军
2015-05-28
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword高寒草甸 高寒湿地 农田 AOA AOB nirK 丰度 群落结构
Abstract本文以三种不同生境的土壤为研究对象,通过构建基因克隆文库与实时荧光定量PCR(qPCR)相结合的方法,调查了三种不同土壤间氨氧化和反硝化微生物丰度、群落结构以及多样性之间的差异,揭示了三种不同土壤类型中氨氧化和反硝化微生物与土壤理化因子之间的相关性。实验所得结果如下:第一:实时荧光定量PCR结果显示,不同生境土壤中硝化和反硝化微生物丰度有显著性差异(p<0.05)。氨氧化古菌和氨氧化细菌的amoA基因拷贝数分别为2.1×107~1.7×109 copies g-1dry soil和7.7×105~1.6×108 copies g-1dry soil。高寒草甸土中AOA的丰度分别是农田和湿地土中的80倍左右,农田土中AOB的丰度分别是湿地和高寒草甸土中的211.8和14.1倍,只有在农田土中AOB的丰度比高,且AOB的丰度是AOA的7.3倍。nirK基因拷贝数为2.1×105~1.4 ×107 copies g-1 dry soil,高寒草甸土中nirK的丰度是农田和湿地土中的11.8和69.2倍。第二:皮森相关性分析表明:AOA的丰度与土壤理化均无显著相关性,而AOB的丰度与含水量成负相关(r=-0.716,p<0.05),与硝态氮(NO3--N)成正相(r=0.928, p<0.01); nirK的丰度与土壤理化因子无显著相关性。第三:CCA分析结果表明,不同生境土壤中硝化和nirK型反硝化微生物群落结构的差异主要是由土壤理化因子引起的。
Other AbstractIn order to explore the response soil type and soil properties to nitrifiers and denitrifiers, this paper investigated the abundance and community structure of nitrifiers and denitrifiers by the combination of clone library analysis and quantitative polymerase clain reaction(qPCR) in three types of soil of different habitats. The results are as follows: Firstly: The results of real-time(qPCR) assays showed that abundances of nitrifiers and denitrifiers are greatly significant(p<0.05). The abundances of Ammonia-oxidizing archaea and Ammonia-oxidizing bacteria amoA gene copies ranged from 2.1×107 to1.7×109 copies g-1 dry soil and 7.7×105~1.6×108 copies g-1 dry soil, respectively. The abundance of AOA amoA gene copies in alphine meadow soil was about 80 fold in agricultural and wetland soil, respectively, and the abundance of AOB amoA gene copies in agricultural soil was respectively 211.8 and 14.1 fold in wetland and alphine meadow soil, while only in the agricultural soil the abundance of AOB was higher than AOA and was 7.3 fold in AOA. The abundance of nirK gene in the alphine meadow soil was respectively 11.8 and 69.2 fold in the agricultural and wetland soil, ranging from 2.1×105 to1.4×107 copies g-1dry soil. Secondly: Pearson’s correlation analysis revealed that no significant relationships were obtained between the abundance of AOA amoA gene and nirK gene and environmental variables (p>0.05). The abundance of AOB amoA gene was negative correlation with soil moisture (r=-0.716,p<0.05), whereas it was positive correlation with NO3--N (r=0.928,p<0.01). Thirdly: The results of CCA analysis showed that differences of nitrifiers and denitrifiers community structure were caused by soil properties between soil of different habitats.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/221678
Collection生命科学学院
Recommended Citation
GB/T 7714
南怀燕. 不同生境土壤中硝化和反硝化微生物群落结构的研究[D]. 兰州. 兰州大学,2015.
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