白血病中XRCC1和CDH13启动子甲基化的研究

兰州大学机构库 >生命科学学院

	白血病中XRCC1和CDH13启动子甲基化的研究
Alternative Title	XRCC1 and CDH13 promoter Methylation in Leukemia
	张婧
Thesis Advisor	杨金波
	2012-05-26
Degree Grantor	兰州大学
Place of Conferral	兰州
Degree Name	硕士
Keyword	白血病甲基化 CDH13 XRCC1 MSP
Abstract	本论文运用甲基化特异性PCR的方法对BJ、K562、HL60三种细胞系以及43例白血病人骨髓样本DNA和20例正常人血液细胞DNA中XRCC1和CDH13基因启动子甲基化进行了研究。在人正常成纤维细胞BJ中，这两个基因均未发生甲基化，在人慢性粒细胞白血病细胞K562中，XRCC1发生了甲基化而CDH13没有发生甲基化，在人急性早幼粒细胞白血病细胞HL60中，这两个基因都发生了甲基化。在白血病人中，这两个基因均有一定程度甲基化，但是在正常人中XRCC1有一定程度甲基化，而CDH13在正常血液样本中没有发生甲基化。因此CDH13具有成为白血病诊断分子标志物的可能性。用DNA甲基化酶抑制剂处理K562细胞后，XRCC1的表达水平上升，证明甲基化可能是抑制其基因表达的一种机制。
Other Abstract	In this study, we mainly use methylation-specific-PCR to detect methylation status of XRCC1 and CDH13 in three cell lines namely BJ, K562, HL60 and 43 leukemia patient samples as well as 20 normal controls. We found that in BJ cells, none of the two genes is methylated and XRCC1 is methylated in K562 cells but CDH13 is not. In HL60 cells, both of the two genes are methylated. In leukemia patients, both of these two genes are methylated to some extent. However, in normal controls, XRCC1 is methylated in some samples and none of the normal samples detected has CDH13 methylation. In conclusion, CDH13 may serve as a biomarker for leukemia detection. Upon DNA methytrasferase inhibitor treatment, expression level of XRCC1 has increased which indicates that promoter methylation might be a mechanism to inhibit XRCC1 expression in K562 cells.
URL	查看原文
Language	中文
Document Type	学位论文
Identifier	https://ir.lzu.edu.cn/handle/262010/221785
Collection	生命科学学院
Recommended Citation GB/T 7714	张婧. 白血病中XRCC1和CDH13启动子甲基化的研究[D]. 兰州. 兰州大学,2012.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

生命科学学院知识库