|Alternative Title||The Effects of TUB4 on Arabidopsis Growth and Development and the Analysis of the New Role of TAO1
|Place of Conferral||兰州
|Other Abstract||In this study, TUB4 and TAO1 were identified as a candidate mutations genes by Map-based cloning. Finally, we confirmed that TUB4 responsible for the abnormal pavement cells shape of ecs1. At the same time, we analysed the novel functions of TAO1 besides disease resistance in Arabidopsis.
The first part mainly study on the mechanism of the effect TUB4 on epidermal cell morphogenesis in Arabidopsis, including the following as:
1. Plant height is decreased, the right-handed helical growth of the stem and root in ecs1.
2. Average area of pavement cells and mesophyll cells are remarkably enlarged in ecs1. Endoreduplication is enhanced in ecs1. The cortical microtubule arrays pattern is altered in hypocotyls epidermal cell of ecs1. Cellulose content is reduced in ecs1, CESA1, CESA4 and CESA5 expression levels is decreased in ecs1.
3. The root gravitropic is weakened in ecs1. The auxin accumulation is reduced in root tip of ecs1, and those of auxin can be restored in root tip with NAA and NPA treatment. PIN1 and PIN2 protein polarity distribution is altered at root cells membrane of ecs1. Many vesiculars are found in root cells, and those vesiculars are insensitivity to BFA treatment in ecs1.
These results showed that TUB4 mutation may affect the vesicles transport in the root, lead to the polarity orientation of PIN1 and PIN2 changed at root cells membrane, finally affect cell morphogenesis and root growth development in ecs1.
In the second part, we analysed the novel functions of TAO1 in Arabidopsis.
TAO1 is a disease resistance protein in plant. Here, we found that TAO1 expression is wider throughout various tissues and organs in Arabidopsis, and that TAO1 protein is localized to both the plasma membrane and chloroplasts. Moreover, we found that TAO1 expression levels was induced by ACC and NAA. We also found that endogenous ethylene production remarkably decreased in TAO1-overexpression lines, but increased in the tao1-10 T-DNA mutant line compared to the wild type, suggesting that TAO1 may be a negative regulator of ethylene signaling. These novel characteristics shed new light on the role(s) of TAO1 in Arabidopsis independent of its role in disease resistance.|
杨建霞. TUB4对拟南芥生长发育的影响和TAO1基因新功能分析[D]. 兰州. 兰州大学,2015.
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