|Alternative Title||Genetic Transformation of PttKN1 Gene and Its Functions Analysis in Petunia hybrida
|Place of Conferral||兰州
|Abstract||PttKN1（Populus tremula × tremuloides KNOTTED1）基因是从杨树的维管束形成层中分离获得的，序列比对揭示它可能属于KNOX基因的第一类基因。 在本研究中，我们利用农杆菌介导的叶盘法已将该基因导入到矮牵牛中，并通过多层次各种水平的分析，探讨了该基因的可能表达模式和功能。
|Other Abstract||PttKN1(Populus tremula × tremuloides KNOTTED1) gene is a new KNOX gene that was isolated from the vascular cambial region of hybrid aspen. Sequence alignment of the gene to a number of plant KNOX genes showed that it belongs to class I KNOX genes. I PttKN1 gene was introduced into Petunia hybrida by Agrobacterinm and its functions had been studied from several aspect in this study.
1.RT-PCR analysis indicated that wild-type Petunia did not express PttKN1 gene but seedlings resistant to kanamycin expressed it.
2.When the PttKN1 gene was overexpressed in Petunia hybrida ,transgenic plant exhibited typical phenotypical features: 1) lobed leaves ,wavy leaves, petioleless leaves, multiform leaf morphology and formation of the ectopic meristems on leaves; 2) defects in internode elongation, stunted plant, severe phenotype was acervate; 3) loss of apical dominance, the plant was scrubby; 4) shortened corolla, dissected corolla, distorted petals, knots projecting from petals with much red color; in addition, some flower organs added in quantity and produce fused flowers, compared with wild type .
3.Analysis of vascular bundle of leaves showed that venation patterns of leaves were abnormal and midvein was shortened, compared to wild type.
4.Analysis of vascular bundle of flower showed that corolla midvein was lost and shortened. The protruding margins of wild-type flowers were changed into concave margins and concave margins of wild-type flowers were changed into protruding margins.
5.The section observation of stem and leaf showed that the vascular cambial region of stem was thicked and ectopic meristems were formed on leaves.
Taken together, our data indicated that PttKN1 gene, a transcript factor, regulates a number of genes which are involved in the formation and maintenance of shoot apical meristem and development of vascular tissue.Therefore, PttKN1 could be very important to plant development, especially it may play a key role in the development of vascular tissue.|
胡鑫. PttKN1基因在矮牵牛中的遗传转化及其功能分析[D]. 兰州. 兰州大学,2002.
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