兰州大学机构库 >生命科学学院
NaCl胁迫下钙添加对蒙古沙拐枣生理特性的影响
Alternative TitleEffects of calcium on physiological characteristics of Calligonum mongolicum Turcz. under salt stress
王文银
Thesis Advisor徐当会
2018-06-04
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword蒙古沙拐枣 NaCl胁迫 钙盐 净光合速率 脯氨酸 丙二醛 叶绿素 灰色关联度
Abstract

不同浓度的NaCl对植物造成不同程度的影响,钙盐在一定水平上能减缓NaCl造成的胁迫。为了探究在相同盐胁迫下,不同种类钙盐的缓解效果以及NaCl与钙盐之间是否存在一个最佳的组合,本试验以荒漠植物蒙古沙拐枣(Calligonum mongolicum Turcz.)为研究材料,采用盆栽法,设计以下处理:(1)对照(CK):不添加NaCl和钙盐;(2)NaCl处理浓度:50、100、200、300 mM;(3)NaCl浓度(0、100、200、300 mM)与Ca(NO3)2浓度(5、10、15、20 mM)交互处理;(4)NaCl浓度(0、100、200、300 mM)与CaCl2浓度(5、10、15、20 mM)交互处理。通过测定以上处理条件下蒙古沙拐枣同化枝中各生理指标的变化情况,使用灰色关联度分析方法分析了各生理指标与地上生物量的关联度,探讨了蒙古沙拐枣的抗盐机理以及钙盐对NaCl胁迫的缓解作用和机制。主要研究结果如下:

少量NaCl(50 mM),同化枝中叶绿素含量及净光合速率(Pn)均增加,说明少量的盐可以促进植物生长;高浓度NaCl(300 mM)使丙二醛(MDA)、脯氨酸(pro)、可溶性蛋白显著性增加,使可溶性糖、叶绿素A、叶绿素B、总叶绿素、Pn显著降低,盐胁迫导致蒙古沙拐枣同化枝光合速率降低是由于叶肉细胞同化能力降低引起的。当钙盐单独处理时,使同化枝内MDA、pro、可溶性糖等生理指标与CK相比增加,使Pn有不同程度的降低,得出钙盐单独处理时同样对植物造成了胁迫。(3)在NaCl胁迫下添加不同浓度Ca(NO3)2对植物体内各生理指标的含量均存在显著的交互作用。NaCl与Ca(NO3)2交互作用时,可提高蒙古沙拐枣同化枝中pro、可溶性蛋白以及可溶性糖的含量,降低MDA的含量,不同的钙盐对叶绿素的缓解作用主要体现在叶绿素A中。(4)在NaCl处理浓度为100和200 mM时,从MDA、pro、Pn、可溶性糖含量可以看出,Ca(NO3)2的添加浓度为10和20 mM时,缓解效果最佳。(5)在相对较低浓度的NaCl胁迫(100 mM)下,低浓度的Ca2+缓解效果更佳,高浓度的Ca2+会加重胁迫;在中高浓度NaCl作用下,则相反。在200和300 mM NaCl处理下,添加相同浓度的Ca(NO3)2与CaCl2,Ca(NO3)2的缓解效果优于CaCl2。(6)灰色关联度分析法得出,不同指标与地上生物量的关联度由大到小依次是:胞间CO2浓度(Ci)> 蒸腾速率(Tr)>净光合速率(Pn)> 气孔导度(Gs) > Chl A/B > pro > 可溶性蛋白 > MDA >可溶性糖 > Chl A > Chl > Chl B。

由上述结果可以得出,少量的NaCl(50 mM)可以促进植物的生长,在盐胁迫条件下,灰色关联度分析结果表明与植物生长关系最紧密的是光合参数指标,高浓度的NaCl影响蒙古沙拐枣的生长,外源钙盐的添加可以缓解NaCl胁迫下对植物造成的损伤,在较低NaCl胁迫下,较低浓度的钙盐缓解效果更佳,在较高NaCl浓度下,则Ca(NO3)2的缓解作用优于CaCl2

Other Abstract

Different concentrations of NaCl caused different damages to the plants, and calcium salts could relieve the stress caused by NaCl to some extent. In order to explore the effects of different types of exogenous calcium salts and the optimal combination of NaCl and calcium under the same salt stress, the desert plant of Calligonum mongolicum Turcz. potted in plastic was used to the research material. The experimental design and profess are as follows: (1) control(CK): not adding any NaCl or calcium; (2) NaCl stress concentrations: 50, 100, 200, 300 mM; (3) interaction between NaCl (0, 100, 200, 300 mM) and Ca(NO3)2 concentrations (5, 10, 15, 20 mM); (4) interaction between NaCl (0, 100, 200, 300 mM) and CaCl2 concentrations (5, 10, 15, 20 mM). By measuring the changes of various characteristics in the assimilation branches of C. mongolicum Turcz. under the above processing conditions, the correlation degree between physiological indexes and above-ground biomass was analyzed using the grey correlation analysis method. The mechanism of salt resistance and the alleviating effect of calcium on NaCl of C. mongolicum Turcz. were discussed. The main results are as follows:

The chlorophyll content and the net photosynthetic rate (Pn) of the assimilating shoots of the C. mongolicum Turcz. were increased with a little of NaCl (50 mM), indicating that a little NaCl could promote the growth of plants; The malondialdehyde (MDA), proline (pro), and soluble proteins content were significantly increased and the soluble sugar, chlorophyll a, chlorophyll b, total chlorophyll, and Pn were significantly decreased at high concentrations of NaCl (300 mM). The decrease of photosynthetic of C. mongolicum Turcz. caused by salt stress is due to the decrease of carboxylation.

When the calcium salt was treated alone, the physiological characteristics MDA, pro, soluble sugar in the assimilation shoots of C. mongolicum Turcz. were increased compared to CK, and the Pn was reduced to some degrees. It was found that the calcium salt alone also caused stress to the plants when treated separately.

Adding Ca(NO3)2 under NaCl stress had a significant interaction with the physiological parameters in plants. When NaCl interacts with Ca(NO3)2, it could increase the content of pro, soluble protein and soluble sugar in the assimilating shoots of C. mongolicum Turcz. and reduce the content of MDA. The effect of different calcium salts on chlorophyll was mainly reflected in chlorophyll A.When NaCl were 100 and 200 mM, it can be seen from the MDA, pro, Pn, and soluble sugar content that adding 10 and 20 mM Ca(NO3)2 had the best mitigation effect. Under low concentration of NaCl stress (100 mM), low concentration of Ca2+ had the better relieves, and high concentration of Ca2+ would aggravate the stress; In the middle and high concentration of NaCl, the opposite was true. Under 200 and 300 mM NaCl treatments, adding the same concentration of Ca(NO3)2 and CaCl2, the mitigation effect of Ca(NO3)2 was better than CaCl2.

The grey relational degree analysis method showed that the correlation degree between physiological indexes and above-ground biomass as follow: intercellular CO2 concentration > transpiration rate > net photosynthetic rate > stomatal conductance > chlorophyll A/B > pro > solubility Protein > MDA > soluble sugar > chlorophyll A > Total chlorophyll > chlorophyll B.

From the above , it can be concluded that a little of NaCl (50 mM) could promote the growth of plants. Under the condition of salt stress, the gray correlation analysis showed that the index of photosynthetic parameters were significantly correlated with above-ground biomass, and the high concentration of NaCl affected the growth of C. mongolicum Turcz. and the addition of exogenous calcium salt could relieve the damage to plants caused by NaCl stress. Under the low NaCl stress, the lower concentration of calcium salt had better remission effect, and at higher NaCl concentration, the mitigation effect of Ca(NO3)2 was better than CaCl2.

URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/221934
Collection生命科学学院
Recommended Citation
GB/T 7714
王文银. NaCl胁迫下钙添加对蒙古沙拐枣生理特性的影响[D]. 兰州. 兰州大学,2018.
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