EDS5及其同源基因EDS5-LIKE参与BAK1和BKK1介导的细胞死亡途径功能的探究

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	EDS5及其同源基因EDS5-LIKE参与BAK1和BKK1介导的细胞死亡途径功能的探究
Alternative Title	Molecular mechanisms of EDS5 and its paralog EDS5-LIKE in regulating BAK1- and BKK1-mediated cell death control pathway
	赵建新
Thesis Advisor	黎家
	2016-05-23
Degree Grantor	兰州大学
Place of Conferral	兰州
Degree Name	硕士
Keyword	BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) 细胞死亡水杨酸 EDS5 EDS5-LIKE 植物免疫
Abstract	在植物适应多种复杂的外界环境中，LRR-RLKs（富含亮氨酸重复序列类受体激酶）在感受细胞外信号并启动细胞内多种反应等方面发挥着极其重要的作用。拟南芥Somatic Embryogenesis Receptor Kinase (SERK)成员从属于LRR-RLKs亚家族，成员包括SERK1、SERK2、SERK3/BAK1、SERK4/BKK和SERK5。它们在油菜素内酯的信号传导、细胞死亡调控及病原菌的抵抗等方面发挥关键作用。BAK1和BKK1的缺失突变体bak1-3 bkk1-1表现出强烈的细胞死亡表型。即使在无菌的培养条件下，bak1-3 bkk1-1双突变体的子叶也出现黄化，并最终导致PR1（病原菌相关基因）表达水平升高、活性氧的爆发以及水杨酸的积累。 EDS5属于MATE-transporter (multidrug and toxin extrusion transporter) 家族，很多研究表明其与水杨酸合成相关。本论文研究目的为探究EDS5及其同源蛋白EDS5-LIKE的功能以及其在BAK1和BKK1介导的细胞死亡途径中的作用。首先进行了EDS5及其同源蛋白EDS5-LIKE的亚细胞定位，然后将这两个蛋白的编码基因超表达到野生型Col-0和C24中，最后将EDS5及其同源基因EDS5-LIKE在bak1-3 bkk1-1的背景下敲除。发现bak1-3 bkk1-1 eds5中的细胞死亡表型相对bak1-3 bkk1-1得到明显缓解，而bak1-3 bkk1-1 eds5-like中的细胞死亡表型与bak1-3 bkk1-1没有明显差异。这些结果表明，EDS5和EDS5-LIKE均定位于叶绿体膜上，且EDS5的功能可能是将在叶绿体中合成的水杨酸运输到细胞质中发挥作用。此外，bak1-3 bkk1-1 eds5可以抑制bak1-3bkk1-1的表型，因此EDS5参与到了bak1-3 bkk1-1介导的细胞死亡途径中，而EDS5-LIKE可能没有参与到此途径中。
Other Abstract	LRR-RLKs play important roles in sensing and transducing extracellular signals under various environmental conditions. Arabidopsis SERKs (Somatic Embryogenesis Receptor Kinases) are the members of the LRR-RLK subfamily. SERKs contain five members, SERK1, SERK2, SERK3/BAK1, SERK4/BKK1 and SERK5. All of them act as crucial regulators in multiple physiological processes including brassinosteroid signaling, cell death control, immunity responses to pathogenic microorganisms. bak1-3 bkk1-1 double mutant shows a cell death phenotype even under a sterile condition. Cotyledons of bak1-3 bkk1-1 seedlings showed yellow color, increased expression of PR genes (pathogenic related genes), outbreak of reactive oxygen species, and accumulation of salicylic acid. EDS5 belongs to MATE-transporter (multidrug and toxin extrusion transporter) family, which is related to the synthesis of salicylic acid (SA). To determine whether EDS5 and paralog EDS5-LIKE are involved in the cell death control pathway mediated by BAK1 and BKK1, we examined the subcellular localization of EDS5 and EDS5-LIKE. We also overexpressed the two genes in Col-0 and C24. Finally we constructed bak1-3 bkk1-1 eds5 and bak1-3 bkk1-1 eds5-like triple mutants. Our detailed analyses indicated that eds5 can significantly suppress the cell death phenotype of bak1-3 bkk1-1. eds5-like, however, cannot suppress the cell death phenotype of bak1-3 bkk1-1. Our results indicated that EDS5 and EDS5-LIKE locate in the envelope of chloroplasts, and EDS5 acts as the transporter of salicylic acid from the chloroplast to cytoplasm. In addition, we conclude that EDS5, but not EDS5-LIKE, is involved in the cell death control pathway mediated by BAK1 and BKK1.
URL	查看原文
Language	中文
Document Type	学位论文
Identifier	https://ir.lzu.edu.cn/handle/262010/221998
Collection	生命科学学院
Recommended Citation GB/T 7714	赵建新. EDS5及其同源基因EDS5-LIKE参与BAK1和BKK1介导的细胞死亡途径功能的探究[D]. 兰州. 兰州大学,2016.

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