兰州大学机构库 >学院待认领
转化生长因子β1对脊髓损伤保护作用的实验研究
Alternative TitleAN EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF TRANSFORMING GROWTH FACTOR BETA 1 FOR SPINAL CORD INJURY
陈文胜
Thesis Advisor孙正义 ; 王栓科
2005-05-31
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword脊髓损伤 慢性脊髓损伤 脊髓缺血再灌注损伤 转化生长因子β1 胶质细胞源性神经营养因子
Abstract目的 通过研究大鼠慢性脊髓损伤及减压后转化生长因子β1及其mRNA的表达变化和大鼠脊髓缺血再灌注损伤后转化生长因子β1及胶质细胞源性神经营养因子的表达变化,探讨转化生长因子β1对脊髓损伤的保护作用。 方法 ①wistar大鼠36只,制备慢性脊髓压迫中、重度及减压后3、7、14d模型,用免疫组化和原位杂交技术 ,观察各组压迫段及压迫邻近段脊髓组织TGF-β1及其mRNA的分布和含量变化。②Wistar大鼠42只,随机分成:正常组、伤后 3、6、12、24、48 h组, 每组 7只。用免疫组化和原位杂交技术,观察大鼠脊髓缺血再灌注损伤后3、6、12、24、48h大鼠脊髓TGF-β1和GDNF的分布和含量变化。 结果 ①慢性脊髓压迫中、重度及减压后3、7d出现大量的TGF-β1;脊髓重度压迫组TGF-β1表达达高峰,减压14天TGF-β1偶见。脊髓神经功能于中、重度压迫组显著下降,减压后逐渐恢复。②脊髓缺血再灌注损伤后3h TGF-β1开始增加,损伤后24h达高峰, GDNF在损伤后6h大量表达,损伤后24h达高峰,以后逐渐下降。脊髓的神经功能于伤后3h显著下降,以后逐渐恢复。  结论 (1)大鼠脊髓损伤过程中机体有内源性保护机制。(2)TGF-β1对脊髓损伤有一定的保护作用。(3)GDNF对脊髓损伤有一定的保护作用。(4)GDNF需要TGF-β1支持其在体内、外的神经营养作用。
Other AbstractObjective To study protective effect of transforming growth factor beta 1 for spinal cord injury through research the expression changes of transforming growth factor beta1,TGF-β1 mRNA after chronic spinal cord injury and the expression changes of transforming growth factor beta1, Glial cell line-derived neurotrophic factor after ischemic reperfusion injury of spinal cord in Rats. Methods ①Thirty-six wistar rats were divided equally into six groups: moderate spinal cord compression group,severe spinal cord compression group and 3 ,7,14 days groups after decompression, normal rats as control groups. The distribution and content changes of TGF-β1 and its mRNA in spinal cord segments were observed by means of immunohistochemistry and in situ hybridization. Neural functional was observed by using grades of Tarlov and inclined plane tests. ② Forty-two wistar rats were randomly divided into normal(n=7), and experimental group(n=35). The rats were give no operation in the normal group, open abdominal and clamping rat abdominal aorta for 40min in the experimental group. Rat were sacrificed at 3、6、12、24 and 48 hours after injury. The distribution and content changes of TGF-β1 and GDNF in spinal cord segments were observed by means of immunohistochemistry and in situ hybridization. Results ①The expression of TGF-β1 was negative in the normal spinal cord. The positive cells of TGF-β1 expressed primarily in moderate compression group,severe compression group and 3 ,7,days groups after decompression. The expression intensity of positive cells in severe compression were (120.33±10.97). The expression intensity of positive cells expressed by mRNA were (86.51±11.75 ) at severe compression. The expression of TGF-β1 was very little in 14 days groups after decompression. Neural functional of spinal cord was declined notablely in moderate compression group and severe compression group, after decompression,it was comeback gradually. ②There was a lot of positive cells of TGF-β1 in spinal cord after ischemic reperfusion injury. TGF-β1 expressed primarily in astrocytes, microglia, macrophages and neuron. Protein began to increase at 3 hours after injury. At 24 hours TGF-β1 and TGF-β1 mRNA expressed to a high level.After that it comedown gradually. There was a lot of positive cells of GDNF after 6h in spinal cord after ischemic reperfusion injury. GDNF expressed primarily in neuron and glial cell. At 24 hours GDNF and GDNF mRNA expressed to a high...
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222165
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
陈文胜. 转化生长因子β1对脊髓损伤保护作用的实验研究[D]. 兰州. 兰州大学,2005.
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