兰州大学机构库 >学院待认领
应用ROC曲线评价P53抗体及核内小核糖体蛋白U1-A抗体对非小细胞肺癌的诊断价值
Alternative TitleEvaluation of the diagnostic value of P53 antibody and small nuclear ribonuelioprotein U1-A antibody to non-
潘辉
Thesis Advisor蔡曦光
2012-05-22
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword蛋白P53 核内小核糖体蛋白U1-A 抗体 非小细胞肺癌 诊断 ROC曲线
Abstract目的:评价P53抗体和U1-AsnRNP(核内小核糖体蛋白U1-A)抗体检测对非小细胞肺癌的诊断价值和临床意义。 方法:采用酶联免疫吸附法(ELISA)检测了46例非小细胞肺癌患者血清中的P53抗体及U1-AsnRNP抗体,并以43例健康人血清作对照。 结果:(1)非小细胞肺癌组血清P53抗体浓度的中位数(四分位数间距)[25.38ng/ml(65.69ng/ml)]明显高于对照组血清P53抗体浓度的中位数(四分位数间距)[10.45ng/ml(10.44ng/ml)],两组间比较差异有统计学意义(P=0.000)。 (2)非小细胞肺癌组血清U1-AsnRNP抗体浓度的中位数(四分位数间距)和对照组血清U1-AsnRNP抗体的浓度的中位数(四分位数间距)分别为165.57U/L(104.51U/L)、170.05U/L(74.12U/L),两组间比较差异无统计学意义(P=0.715)。 (3)应用ROC曲线对P53抗体和U1-AsnRNP抗体对非小细胞肺癌的诊断价值进行了评价,ROC曲线下面积分别71.7%、47.8%,P53抗体诊断非小细胞肺癌的敏感性和特异性分别为62.2%、79.1%。 结论:(1)P53抗体对非小细胞肺癌的诊断有良好的敏感性和特异性,血清P53抗体检测对非小细胞肺癌诊断有重要的临床意义。 (2)U1-AsnRNP抗体对非小细胞肺癌的诊断价值欠佳。 关键词:蛋白P53;核内小核糖体蛋白U1-A;抗体;非小细胞肺癌;诊断;ROC曲线
Other AbstractObjective:To evaluate the diagnostic value and clinical significance of the detection of P53 antibody and U1-AsnRNP (small nuclear ribosomalprotein U1-A) antibody to NSCLC. Methods:Forty-six patients with NSCLC were tested for P53 antibodies and U1-AsnRNP antibodies by enzyme linked immunosorbent assay( ELISA),Forty-three healthy persons were recruited as controls. Results:(1)The concentration of serum P53 antidbody in patients was obviously higher than the concentration of control group[25.38ng/ml(65.69ng/ml)and 10.45ng/ml(10.44ng/ml)]. There was statistic significant difference between the two groups (P = 0.000). (2)The concentration of serum U1-AsnRNP antidbody in patients was 165.57U/L(104.51U/L), and it was 170.05U/L(74.12U/L)in control group. There was no statistic significant difference between the two groups(P=0.715). (3)ROC curve analysis was done in order to evaluate their diagnostic value to NSCLC,which showed the area under the ROC curve( AUC) of P53 antibody and U1-AsnRNP antibody were 71.7% and 47.8 %. P53 antibody sensitivity and specificity of diagnosis to NSCLC were 62.2% and 79.1%,respectively. Conclusions:(1)The P53 antibody for the diagnosis of NSCLC demonstrated good sensitivity and specificity,and the decection of serum P53 antibody has important clinical significance in diagnosis of NSCLC. (2)The U1-AsnRNP antibody has poor value in diagnosis of NSCLC. Key words:Protein P53;Small nuclear ribonuelioprotein U1-A Antibody; Non-small cell lung cacer; Diagnose;ROC curve
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222314
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
潘辉. 应用ROC曲线评价P53抗体及核内小核糖体蛋白U1-A抗体对非小细胞肺癌的诊断价值[D]. 兰州. 兰州大学,2012.
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