| Other Abstract | Objective :This study aims to establish the CPR model in rabbits in order to demonstrate the neuroprotective effects of ethanol post-cardiac arrest.
Methods: 40 rabbits were randomly divided into five groups: sham group, the control group, 0.5g/kg group, 1.0g/kg group, and 1.5g/kg group. After the estabilshment of CPR model , those rabbits were injected with different doses of ethanol immediately following the restoration of spontaneous circulation. After 2h, 8h and 12h, S-100 protein expression were tested respectively in order to determine the dose–response effect of ethanol in brain necrosis volume.
Results:
1 The brain necrosis volume of different groups. The brain necrosis volume of the sham group, the control group, the 0.5g/kg ethanol group, the 1.0g/kg ethanol group, and the 1.5g/kg ethanol group are 5.26±0.26%,, 29.2 ± 1.46%, 25.0 ± 1.25%, 20.4 ± 1.02%, and 15.3 ±0.76% respectively. Among the four groups, 1.5g/kg ethanol group has the most significant neuroprotective effect （p<0.001）.
2 Serum S-100 concentration of the different groups after 2h, 8h and 12h.
After 2h, Serum S-100 concentration of the sham group, the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, and the control group are 1.19 ± 0.05μg / L, 1.04 ± 0.05μg / L, 0.35 ± 0.01μg / L, 2.25 ± 0.11μg/L and 0. 06 ±0.03μg/L respectively. S-100 concentration of the 1.5g/k group is the lowest one （p<0.001）.
After 8h, S-100 concentration of the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, the control group and the sham group, are 3.96 ± 0.19μg / L, 2.12 ± 0.10μg / L, 0.45 ± 0.02μg / L, and 4.03 ± 0.20μg / L and 0. 06 ±0. 06μg/L respectively. The S-100 concentration of the 1.5g/kg group is lowest one （p <0.001）.
After 12h, the S-100 concentration of the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, the control group and the sham group are 5.58 ± 0.27μg / L, 4 96 ± 0.24μg / L, 0. 98 ± 0.04μg / L, 7. 25 ± 0.36μg /L and 0. 06 ±0. 01μg/L respectively. The serum S-100 concentration of the 1.5g/kg group is the lowest one （p <0.001）.
Conclusion: The result of the experiment suggests that an immediate injection of a dose of 1.5g/kg ethanol in rabbits immediately following the restoration of spontaneous circulation have neuroprotective effect.
Keywords: Post-cardiac arrest syndrome, cerebral resuscitation, ethanol, S-100, brain protection. |
