兰州大学机构库 >学院待认领
乙醇对家兔心脏骤停后综合征脑保护的实验研究
Alternative TitleNeuroprotective Effect of Acute Ethanol in Rabbits with Post-Cardiac arrest syndrome
魏林
Thesis Advisor李培杰
2013-05-22
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword心脏骤停后综合征 脑复苏 乙醇 脑保护
Abstract目的:本研究的目的是建立家兔心肺复苏模型,论证乙醇对心脏骤停后综合征的脑保护作用。 方法:40只家兔随机分入5组,分别是:假手术组,对照组,0.5g/kg乙醇组,1.0g/kg乙醇组,1.5g/kg乙醇组。建立家兔心肺复苏动物模型,自主循环恢复后即刻给予不同剂量乙醇。分别于2h,8h,12h抽血检测血清S-100蛋白浓度,存活12小时后,处死比较脑坏死体积。 结果:1.假手术组、对照组、0.5g/kg组、1.0g/kg组、1.5g/kg组脑坏死体积分别是5.26±0.26、29.2±1.46%、25.0±1.25%、20.4±1.02%、15.3±0.76%,由此可见1.5g/kg组脑坏死体积最小(p<0.001)。 2.自主循环恢复后2h、8h、12h分别监测 5组家兔血清S-100蛋白含量。 2h时,0.5g/kg组、1.0g/kg组、1.5g/kg组、对照组、假手术组,血清S-100浓度分别是1.19±0.05μg/L,1.04±0.05μg/L,0.35±0.01μg/L,2.25±0.11μg/L,0.06 ±0.03μg/L其中,1.5g/kg组血清S-100浓度最低(p<0.001)。 8h时,0.5g/k乙醇组,1.0g/kg组,1.5g/kg组,对照组,假手术组血清S-100浓度分别为3.96±0.19μg/L,2.12 ±0.10μg/L,0. 45±0.02μg/L,4.03±0.20μg/L,0.06 ±0.06μg/L。其中1.5g/kg乙醇组血清S-100浓度最低(p<0.001)。 12h时,0.5g/kg组,1.0g/kg组,1.5g/kg组,对照组,假手术组血清S-100浓度分别为5.58±0.27μg/L,4.96 ±0.24μg/L,0. 98 ±0.04μg/L,7.25 ±0.36μg/L,0. 06 ±0.01μg/L。其中1.5g/kg组血清S-100浓度最低(p<0.001)。 结论:1.5g/kg乙醇在家兔自主循环恢复后即刻注射具有脑保护作用。
Other AbstractObjective :This study aims to establish the CPR model in rabbits in order to demonstrate the neuroprotective effects of ethanol post-cardiac arrest. Methods: 40 rabbits were randomly divided into five groups: sham group, the control group, 0.5g/kg group, 1.0g/kg group, and 1.5g/kg group. After the estabilshment of CPR model , those rabbits were injected with different doses of ethanol immediately following the restoration of spontaneous circulation. After 2h, 8h and 12h, S-100 protein expression were tested respectively in order to determine the dose–response effect of ethanol in brain necrosis volume. Results: 1 The brain necrosis volume of different groups. The brain necrosis volume of the sham group, the control group, the 0.5g/kg ethanol group, the 1.0g/kg ethanol group, and the 1.5g/kg ethanol group are 5.26±0.26%,, 29.2 ± 1.46%, 25.0 ± 1.25%, 20.4 ± 1.02%, and 15.3 ±0.76% respectively. Among the four groups, 1.5g/kg ethanol group has the most significant neuroprotective effect (p<0.001). 2 Serum S-100 concentration of the different groups after 2h, 8h and 12h. After 2h, Serum S-100 concentration of the sham group, the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, and the control group are 1.19 ± 0.05μg / L, 1.04 ± 0.05μg / L, 0.35 ± 0.01μg / L, 2.25 ± 0.11μg/L and 0. 06 ±0.03μg/L respectively. S-100 concentration of the 1.5g/k group is the lowest one (p<0.001). After 8h, S-100 concentration of the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, the control group and the sham group, are 3.96 ± 0.19μg / L, 2.12 ± 0.10μg / L, 0.45 ± 0.02μg / L, and 4.03 ± 0.20μg / L and 0. 06 ±0. 06μg/L respectively. The S-100 concentration of the 1.5g/kg group is lowest one (p <0.001). After 12h, the S-100 concentration of the 0.5g/kg group, the 1.0g/kg group, the 1.5g/kg group, the control group and the sham group are 5.58 ± 0.27μg / L, 4 96 ± 0.24μg / L, 0. 98 ± 0.04μg / L, 7. 25 ± 0.36μg /L and 0. 06 ±0. 01μg/L respectively. The serum S-100 concentration of the 1.5g/kg group is the lowest one (p <0.001). Conclusion: The result of the experiment suggests that an immediate injection of a dose of 1.5g/kg ethanol in rabbits immediately following the restoration of spontaneous circulation have neuroprotective effect. Keywords: Post-cardiac arrest syndrome, cerebral resuscitation, ethanol, S-100, brain protection.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222364
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
魏林. 乙醇对家兔心脏骤停后综合征脑保护的实验研究[D]. 兰州. 兰州大学,2013.
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