兰州大学机构库 >学院待认领
血浆D-乳酸和二胺氧化酶对小儿胃肠功能障碍评价的研究
Alternative TitleStudy on plasma D-lactate and DAO to evaluate the children's GI dysfunction
张妮
Thesis Advisor朱保权 ; 张庆立
2005-05-31
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
KeywordD-乳酸 二胺氧化酶 胃肠功能障碍
Abstract目的: 探讨小儿危重症血浆D-乳酸和二胺氧化酶(DAO)的变化规律,评价血浆D-乳酸和DAO活性对小儿危重症胃肠功能障碍的诊断价值。 方法: 收集兰大二院儿科PICU2003年11月至2004年11月收住的62例患儿的血浆标本。采用分光光度法检测不同危重程度,胃肠功能障碍或衰竭时血浆D-乳酸和DAO的水平以及动态监测小儿危重症急性期和恢复期D-乳酸和DAO活性的变化,并与20例正常小儿进行比较研究。 结果: 1.随着危重程度增加,血浆D-乳酸显著上升,不同危重程度组血浆D-乳酸水平相比差异有统计学意义(F=21.72,P<0.01);组间比较,危重组、极危重组血浆D-乳酸较对照组和非危重组明显升高,差异有统计学意义(P<0.01);极危重组血浆D-乳酸较危重组明显升高,差异有统计学意义(P<0.01);非危重组血浆D-乳酸较对照组差异无统计学意义(P>0.05)。不同危重程度组血浆DAO活性比较极危重组较对照组下降,差异有统计学意义 (P<0.05)。2.胃肠功能损害加重,血浆D-乳酸升高差异有统计学意义(F=16.28,P<0.01)。不同胃肠功能状态患儿血浆DAO活性比较差异无统计学意义(F=2.36,P>0.05)。3.危重组及极危重组患儿恢复期血浆D-乳酸水平较急性期明显下降,差异有统计学意义(分别为t=6.25, P<0.01;t=8.40,P<0.01)。危重组、极危重组患儿急性期与恢复期血浆DAO活性比较差异无统计学意义(t=1.32,P>0.05)。 结论: 血浆D-乳酸水平与小儿危重症危重程度,胃肠功能损害程度密切相关。血浆D-乳酸可作为小儿危重症胃肠功能障碍的诊断指标,并可作为动态监测小儿危重症胃肠功能修复的指标。血浆DAO活性与小儿危重症胃肠功能之间的关系有待深入研究。
Other AbstractSTUDY ON PLASMA D-LACTATE AND DIAMINE OXIDASE TO EVALUATE THE CHILDREN’S GASTROINTESTINAL DYSFUNCTION Objective: To investigate the changes of plasma D-lactate level and diamine oxidase(DAO) activity in the critical children. To explore the value of D-lactate and DAO in measuring the intestinal barrier function, judging the course of disease. Methods: 62 Patients aged from 1 month to 14 years (averaged 3.9 years) including 44 boys and 18 girls were divided into no-critical group, critical group and extreme critical group as defined by Children’s Critical Disease Assessment Scale. Furthermore, all patients were divided into no-gastrointestinal dysfunction group and gastrointestinal dysfunction group basing on the patients with or without GI tract dysfunction, and 20 normal children were performed as control group. For statistical analysis the SPSS software package (version 8.0) was used. Statistic significance was set atP<0.05. Plasma D-lactate level and DAO activity were detected by spectrophotometric assay. Results: 1. The mean of plasma D-lactate in different critical groups was significant dramatically(F=21.72,P<0.01); D-lactate concentrations in extreme critical group increased dramatically than that of other groups(P<0.01). There was no relationship between DAO activity and severity of disease. 2. Plasma D-lactate levels in gastrointestinal dysfunction group were higher than that of normal group and no-gastrointestinal dysfunction group (F=19.59,P<0.01); 3. Plasma D-lactate levels in the recovered phase decreased significantly than that of the acute phase(t=6.25, t=8.40,P<0.01 respectively).There was no significant difference of DAO activity in different phases and different states of GI function. Conclusions: Plasma D-lactate may be more closely related to the development of GI dysfunction or failure and severity of disease. It may be a useful marker to evaluate and monitor gastrointestinal function. But the relationship between DAO activity and GI function needs to be further studied.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222431
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
张妮. 血浆D-乳酸和二胺氧化酶对小儿胃肠功能障碍评价的研究[D]. 兰州. 兰州大学,2005.
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