兰州大学机构库 >学院待认领
新生儿缺氧缺血性脑病患儿血清白细胞介素18和神经元特异性烯醇化酶含量测定及临床意义
Alternative TitleDetection and clinical significance of IL-18 and NSE in serum of newborns with Hypoxic- ischemic encephalopathy
袁二伟
Thesis Advisor余唯琪
2009-05-17
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword缺氧缺血,脑 白细胞介素-18 神经元特异性烯醇化酶 酶联免疫吸附试验
Abstract目的:研究新生儿缺氧缺血性脑病患儿血清白细胞介18和神经元特异性烯醇化酶的变化、相关性及临床意义,进行新生儿行为神经测定并探讨IL-18损伤脑的作用机制。 方法:选择2007年9月至2008年5月兰州大学第一医院NICU收治的HIE患儿33名,同期兰州大学第一医院出生的正常足月新生儿20名。HIE患儿在生后第1天和生后第7天早晨喂奶前由专人分别股静脉采血2ml,对照组的新生儿在生后第1天股静脉穿刺取血2ml,常温2000r/min离心10min后分离血清。采用ELISA法,在相同的条件下检测血清标本中的IL-18和NSE的含量。 结果:HIE组血清IL-18和NSE明显高于对照组,结果具有统计学意义( P均<0.01);HIE各组间血清IL-18和NSE均进行两两比较,中度组与轻度组比较差异有统计学意义(P均<0.05),重度组与其他两组比较差异均有统计学意义(P均<0.01);HIE各组生后第1天与生后第7天血清IL-18和NSE分别进行比较,中、重度组差异均有统计学意义(P<0.01,<0.05);生后第1天,HIE组血清IL-18和NSE呈正相关(r=0.832,P=0.000),IL-18和NSE与NBNA评分均呈负相关(r=-0.557,P=0.001;r=-0.496,P=0.003)。 结论:HIE患儿血清IL-18和NSE的浓度明显高于对照组,并与其临床分度基本一致。监测HIE患儿血清IL-18和NSE水平,对疾病早期诊断、病情程度的判断、治疗效果的评价以及预后判定具一定的临床价值
Other AbstractObjectives: The aim of this experiment is to study the changes of serum concentrations of Interleukin-18and Neuron-specific enolasein newborns with Hypoxic-ischemic encephalopathy, the correlation between IL-18 and NSE , and between IL-18 and NSE and Neonatal behavioral neurological assessment, and clinical significance. To explore the mechanism of brain injury caused by IL-18. Methods: 33 infants with HIE were choosed who were treated in Lanzhou University First Hospital NICU from September 2007 to May 2008. Twenty term normal newborns worked as control group. Infants with HIE were separately collect the femoral vein blood 2ml by special messengers before feeding in the morning on the first day and the seventh day after birth ,the control group were collect the femoral vein blood 2ml on the first day after birth .The bloood were centrifuged for 10 minutes at room temperature in 2000r/min .Serum concentrations of IL-18 and NSE were measured by enzyme-linked immunosorbent assay under the same conditions. Result: Serum concentrations of IL-18 and NSE in infants with HIE were significantly higher than those in control group(P<0.01).Both serum IL-18 and NSE concentrations were associated with the severity of HIE except mild group(P<0.01).Severe HIE newborns had the highest serum IL-18 and NSE levels,followed by moderate HIE newborns. Serum concentrations of IL-18 and NSE in moderate and severe HIE neonates in recovery period were significantly lower than those in acute period(P<0.01,<0.05). In acute period , there was a significant positive correlation between IL-18 and NSE (r=0.832,P=0.000), there was a significant negative correlation between IL-18 and NBNA(r=-0.557,P=0.001), and there was a negative correlation between NSE and NBNA(r=-0.496,P=0.003). Conclusions: The levels of IL-18 and NSE in HIE infants were approximately higher than those in control group, and they were identical with the severity of HIE,so they may be useful makers for the diagnosis of HIE.The measurement of IL-18 and NSE will be very important to the early diagnosis and jugement of therapeutic efficacy and the prognosis.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222459
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
袁二伟. 新生儿缺氧缺血性脑病患儿血清白细胞介素18和神经元特异性烯醇化酶含量测定及临床意义[D]. 兰州. 兰州大学,2009.
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