| 新生儿败血症核因子-Kappa B表达水平及临床意义 |
| Alternative Title | The significance on expression of nuclear factor-κB in neonatal sepsis
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| 倪倩 |
| Thesis Advisor | 朱保权
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| 2005-05-20
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 新生儿败血症
核因子-Kappa B
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| Abstract | 目的 目前研究认为炎性细胞因子如白介素（IL）-1、6、8等在新生儿败血症发病早期高水平表达而可以作为早期诊断指标。核因子-κB（nuclear factor-κB，NF-κB）作为炎性细胞因子的上游调节因子是否也在新生儿败血症中高水平表达不得而知。为寻找一个敏感的早期临床诊断指标以指导临床用药，本实验将探讨NF-κB表达水平与新生儿败血症的关系。
方法 参照《新生儿败血症诊疗方案》及血培养结果将患儿分为败血症组（26例）和非败血症组（31例）。对照组为正常新生儿（20例）。采用流式细胞仪分别检测患儿入院时、入院后24及48小时外周血单个核细胞（PBMC）中NF-κB的表达；每位患儿在入院时使用抗生素前取血做血培养。
结果 败血症组PBMC中NF-κB表达明显高于非败血症组和对照组（P<0.01），而非败血症组与对照组比较无统计学意义（P=0.285）；败血症组NF-κB入院时明显高于入院后24、48小时（分别为16.87±5.05，9.15±2.47，7.68±2.52，P<0.0001），并且在治疗后24小时表达水平显著下降，24小时与48小时比较无显著差异（P>0.05）；而非败血症组在入院时、入院后24及48小时NF-κB的表达水平无显著差异, P>0.05。
结论 1、NF-κB在新生儿败血症早期表达显著增加并随治疗动态变化，提示NF-κB可作为败血症患儿的早期诊断指标并指导临床治疗。2、流式细胞技术检测NF-κB具有较好的敏感性和特异性，且操作简便、快捷，有较好的临床实用价值。3、结合NF-κB检测，选择其抑制剂应用于临床必将对败血症的治疗及改善其预后产生重要的实际意义。4、关于NF-κB对新生儿败血症诊断的预测价值和预后的判断价值以及提高检测技术的准确性，开发、筛选NF-κB抑制剂乃至选择性抑制剂等都将需要进一步深入研究。
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| Other Abstract | Objective Inflammatory cytokines (such as interleukine-1, 6, 8 etc.)have been used , as a trial, in clinical diagnosis. In order to find whether nuclear factor-κB(NF-κB) which has been the up-regulator of inflammatory cytokines expresses in peripheral mononuclear cells ( PBMC) of neonatal sepsis and how it functions in neonatal sepsis, we plan to investigate the expression and the effect of NF-κB in neonatal sepsis.
Methods We separated 77 newborn infants into 3 groups, which are septic group (26 cases), non-septic group (31 cases) and control group (20 cases). NF-κB existed in PBMC was detected in 3 different periods, including at admission, after the 24th hour and 48th hour of admission, of each group by flow cytometry. At the same time, the sample of the first two groups were drawn for blood cultures at admission before using antibiotics.
Results The expression of NF-κB detected in septic group was more significant than that detected in the other two groups (P<0.01). And there was no significant difference between the non-septic group and the control group. Moreover, the expression of NF-κB, in septic group, increased at admission and decreased after the 24th hour, but it was no difference between the 24th hour and 48th hour. And, the expression of NF-κB was also no significant difference at the 3 different periods in non-septic group.
Conclusions It has been demonstrated that NF-κB increased in early period of neonatal sepsis, and not only can be used as a monitor standard in the early diagnosis of neonatal sepsis, but also can direct the therapy. Furthermore, the flow cytometry used in the NF-κB detecting, which considered as a sensitive and convenient method, can easily be used in most hospitals. Whether or not such an expression of NF-κB has the predictive value in diagnosis and prognosis needs further studies.
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| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222462
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
倪倩. 新生儿败血症核因子-Kappa B表达水平及临床意义[D]. 兰州. 兰州大学,2005.
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