兰州大学机构库 >学院待认领
戊乙奎醚后处理和远端缺血后处理对肢体缺血再灌注大鼠肝损伤的影响
Alternative TitleComparison of the effects of penehyclidine and remote ischemic post-conditioning on rat liver after lower-limb-ischaemia-reperfusion injury
张悦
Thesis Advisor冷玉芳
2011-05-17
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword再灌注损伤 肝脏 戊乙奎醚 炎症 活性氧 缺血后处理 缺血预处理
Abstract目的 观察不同再灌注时间对肢体缺血再灌注大鼠肝脏的影响;探讨比较戊乙奎醚后处理及远端缺血后处理在抑制氧化应激和抗炎方面对肢体缺血再灌注大鼠肝脏损伤的作用。 方法 健康成年雄性Wistar大鼠144只,体重220-250g,随机分为4组:对照组(C组)、肢体缺血再灌注组(LIR组)、戊乙奎醚后处理组(PHC组)及远端缺血后处理组(RIPO组),每组根据再灌注时间的不同又随机分为6个亚组(n=6):T0(0h)、T1(1h)、T2(3h)、T3(6h)、T4(12h)、T5(24h)。肢体缺血再灌注模型是通过弹力橡皮筋结扎双后肢,完全阻断血流3h后,立即剪开橡皮筋,恢复双后肢血流,分别再灌注一定时间实现的。PHC组在完全阻断大鼠双下肢血流2h57min自尾静脉注射戊乙奎醚0.15mg/kg, RIPO组缺血2h57min后,用弹力橡皮筋在双下肢进行三次短暂的(30s)复灌、(30s)缺血循环。测定血清血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性和肝脏病理改变评估肝功能;检测肝组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性以反映肝组织氧化损伤程度;测定TNF-α、IL-10含量、血浆内毒素浓度及肝组织髓过氧化物酶(MPO)活性反映炎症水平。 结果 与C组比较, LIR组血清ALT、AST活性T1~T5升高,PHC组血清ALT活性T2~T5升高,RIPO组血清ALT活性T1~T5升高,两组血清AST活性T1~T4升高,三组MDA含量在T1、T3升高,LIR组及RIPO组肝组织SOD活性T0~T5下降,PHC组肝组织SOD活性T1~T5下降,三组肝组织MPO活性T1~T4升高,三组血浆内毒素浓度及血清IL-10含量T0~T5升高,LIR组血清TNF-α含量在T0、T1升高,PHC组及RIPO组血清TNF-α含量在T1升高(P<0.05);与LIR组比较,PHC组血清ALT活性在T1~T3下降,RIPO组血清ALT活性T3下降,PHC组及RIPO组血清AST活性T1~T4下降,两组MDA含量于T1、T3下降,两组肝组织SOD活性T1~ T5升高,两组肝组织MPO活性在T1~T3下降,两组血浆内毒素浓度T1~T5下降,两组血清TNF-α含量T1降低,两组血清IL-10含量T1~T4升高(P<0.05);与PHC组比较,RIPO组血清ALT、AST活性T2升高,RIPO 组肝组织SOD活性T1~T2较低,RIPO组血浆内毒素浓度T1、T3~T5升高,RIPO组血清TNF-α含量T1升高,RIPO组血清IL-10含量T2~T4升高(P<0.05)。光镜下,C组肝小叶结构完整,肝细胞呈多边形,胞浆着色均匀;肝血窦呈透明间隙。LIR组T2、T3肝索排列紊乱或出现断裂,肝窦扩张,部分有充血;胞浆疏松化,空泡变性,甚至出现气球样变和嗜酸性变。PHC组及RIPO组T2、T3肝小叶结构基本完整,肝细胞无明显变性,肝窦排列相对整齐。 结论 下肢缺血再灌注可以引起肝脏损伤;戊乙奎醚后处理及远端缺血后处理均可减轻肢体缺血再灌注对大鼠肝脏的损伤;戊乙奎醚后处理的效果强于远端缺血后处理。
Other AbstractObjective To investigate and compare the effect of post-conditioning with penehyclidine and remote ischemic on liver peroxidation and inflammation in lower-limb-ischaemia-reperfusion rats. Methods One hundred and forty-four healthy male Wistar rats weighing 220-250g were randomly divided into four groups: control group (group C),lower-limb- ischemia-reperfusion group (LIR group), penehyclidine post-conditioning group (PHC group) and remote ischaemia post-conditioning group (RIPO group). Based on the duration of reperfusion, the rats in each group were randomly assigned to six sub-groups (n=6), no reperfusion (T0), reperfusion for 1 (T1),3 (T2),6 (T3),12 (T4),24hours(T5). Lower-limb-ischaemia-reperfusion was performed by applying rubber bands at the bilateral trochanter major level for 3 hours, followed by reperfusion except in group C. After 2 hours and 57minutes of lower-limb ischaemia, penehyclidine was injected into the rats through the tail vein in PHC group, whereas the rats in IPO group were subjected to three episodes of 30-s ischemia at 30-s intervals. Liver function was evaluated by serum alanine transaminase (ALT), aspartate transaminase (AST) activities and hepatic pathology. Oxidative injury was assessed by malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of the liver. Inflammatory level was reflected by myeloperoxidase (MPO) activity of the liver, tumour necrosis factor (TNF)-α, interleukin (IL)-10 levels in the serum and endotoxin concentration in the plasma. RESULTS Both the oxidative and inflammatory indices significantly increased after lower-limb-ischaemia-reperfusion. Furthermore, they were suppressed by either penehyclidine or remote ischaemic post-conditioning. Compared with group C, serum ALT and AST activities in group LIR were significantly higher from T1 to T5, serum ALT activity in group PHC was significantly higher from T2 to T5, serum ALT activity in group RIPO was significantly higher from T1to T5, and serum AST activity in group PHC and group RIPO was significantly higher from T1 to T4(P<0.05), in other three groups, MDA content in liver was significantly higher at T1 and T3(P<0.05), SOD activity of liver in group LIR and RIPO was significantly lower from T0 to T5, while in group PHC it was significantly lower from T1 to T5(P< 0.05), in other three groups, MPO activity in liver was significantly higher from T1 to T4(P< 0.05), plasma endotoxin concentration and serum IL-10 assay was higher...
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222534
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
张悦. 戊乙奎醚后处理和远端缺血后处理对肢体缺血再灌注大鼠肝损伤的影响[D]. 兰州. 兰州大学,2011.
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