兰州大学机构库 >学院待认领
无精症患者血清、精浆性激素水平与生精细胞检测的相关性研究.
Alternative TitleThe Purpose of The Study on Relation Between Sexual Hormone Detection in blood serum and Seminal Plasma and Spermatogenic Cells Test in Patients with Aspermia
田英
Thesis Advisor丁进芳
2008-05-27
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword无精子症 生精细胞 精浆 血清 性激素
Abstract目的 1.血清、精浆性激素水平的变化与无精症相关性的研究;2.无精症血清性激素和精浆性激素检测的临床诊断价值的研究;3.探讨无精子症患者精浆PH值、性激素水平和生精细胞检测的相关性。 方法 1.选择60例无精子症患者根据精液PH值分为:高 PH值组38例(pH值7.0~8.0)低pH值组22例(pH值<7.00);另以20例正常男性作为对照组。2.采用电化学发光免疫法(ECLI)测定精浆睾酮(T)、卵泡刺激素(FSH)、泌乳素(PRL)水平,血清性激素水平除上述三项激素外又增加黄体生成素(LH)的测定。3.将精液以3000转/min离心15min后沉渣涂片,自然干燥后瑞-姬染色,油镜(X1000)进行生精细胞学检测。 结果 1.高PH值组38例无精症组中精浆睾酮(T)、卵泡刺激素(FSH)与对照组相比,差异均有统计学意义(P<0.05),低PH值组22例精浆中T、FSH与对照组相比差异均无统计学意义(P >0.05)。无论是高PH值组还是低PH值组精浆中泌乳素(PRL)与对照组相比差异无统计学意义(P>0.05)。2.高PH值组38例无精症组中血清睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)与对照组相比,差异均有统计学意义(P<0.05),低PH值组22例血清中T、FSH、LH与对照组相比差异均无统计学意义(P>0.05)。无论是高PH值组还是低PH值组血清中泌乳素(PRL)与对照组相比差异无统计学意义(P>0.05)。3.生精细胞形态学检查对照组20例男性镜下均可发现正常生精细胞及发育成熟精子;高pH组38例患者中33例发现生精细胞,主要停滞于次级精母细胞和精子细胞阶段,低PH值组22例均未发现生精细胞。 结论 1.高PH值组38例无精症患者血清、精浆性激素与对照组间有统计学差异,结合精液细胞学检查可以推测此组主要由于睾丸原发性病变引起激素分泌水平异常所致。2.低PH值组22例血清、精浆FSH、T水平与对照组间无统计学差异,同时精液细胞学检查均未见生精细胞,说明性腺功能正常,这种无精子症患者主要是由于单纯性输精管阻塞而产生。
Other AbstractObjective:1.The change of sexual hormone in blood serum and seminal plasma and the study of pathogenetic mechanism;2.The mutual ralationship of sexual hormone in blood serum and seminal plasma and the study of diagnostic value;3.To detect the sexual hormone in seminal plasma and analyze the spermatogenic cells of the patients with aspermia. Method: 1.According to the account of PH of seminal plasma, 60 patients of aspermia were divided into high PH group adding up to 38 ones (PH 7.2-8.0) and low PH group 22 ones (PH<7.0). Then 20 normal men were choosed as the control group. 2.Electrochemistry luminescence immunity(ECLI)was used to detect sexual hormone in blood serum and seminal plasma. 3.Wright-Giemsa stain was used to test the spermatogenic cells in 60 aspermia patients and men of control group. Result:1.In high PH group, spermatogenic cell could be detected in 33 patients. And then there was statistic difference between high PH group and the control group in the follicle stimulating hormone (FSH) and testosterone(T) in seminal plasma (P<0.05). spermatogenic cell can be not detected in all of 22 examples in low PH group . There was no statistic difference between low PH group and the control group in the FSH and T in seminal plasma (P>0.05).2. In high PH group, spermatogenic cell could be detected in 33 patients. And then there was statistic difference between high PH group and the control group in the follicle stimulating hormone (FSH) and testosterone(T) in blood serum (P<0.05). spermatogenic cell can be not detected in all of 22 examples in low PH group . There was no statistic difference between low PH group and the control group in the FSH and T in seminal plasma (P>0.05). Conclusion1.comprehensive analysis of PH、sexual hormone and spermatogenic cell test in aspermia can correctly diagnose the cause of aspermia and have important significance in commanding the clinical treatment. 2. In high PH group, spermatogenic cell could be detected in 33 patients,can be inferred primary testicle process cause abnormal hormone secretion.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222538
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
田英. 无精症患者血清、精浆性激素水平与生精细胞检测的相关性研究.[D]. 兰州. 兰州大学,2008.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[田英]'s Articles
Baidu academic
Similar articles in Baidu academic
[田英]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[田英]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.