| 胃癌组织中ENO1、M2-PK的表达与临床病理意义 |
| Alternative Title | The expression of ENO1 and M2-PK in human gastric carcinomas and its clinical significance
|
| 倪田根 |
| Thesis Advisor | 关泉林
|
| 2011-05-18
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 胃癌
烯醇化酶-α
肿瘤型丙酮酸激酶
免疫组化
|
| Abstract | 目的 探讨烯醇化酶-α（enolase-α,ENO1)、肿瘤型丙酮酸激酶(tumor M2 pyruvate kinase,M2-PK)在胃良性病变组织及胃癌组织中的表达，分析其在胃癌组织中的相互关系及其临床病理学意义。
方法 应用免疫组织化学SP染色方法分别对55例胃癌组织和23例胃良性病变组织切片染色，观察胃癌组织和良性病变组织中ENO1、M2-PK的表达情况，比较两组间的差异。胃癌组再按年龄、性别、分化程度、浸润深度、淋巴结转移、临床分期等参数进行分组比较，观察胃癌组织中ENO1、M2-PK的表达情况及分析其临床病理意义。
结果
1、胃癌组织中ENO1阳性表达率为67.3% (37／55)，明显高于胃良性病变组30.4％(7／23) ( P<0.01)，其表达与胃癌分化程度、浸润深度、淋巴结转移及TNM分期均显著相关(均P<0.05)。
2、胃癌组织中M2-PK的阳性表达率为78.2％(43／55)，明显高于胃良性病变组织39.1％(9／23) ( P<0.01)，其表达与胃癌分化程度、浸润深度均显著相关(均P<0.05)。
3、胃癌组织中ENO1与M2-PK的表达呈正相关(r=0.5729，P<0.05)。
结论 胃癌组织中ENO1和M2-PK高表达与胃癌的发生、发展可能有关，两者在胃癌的侵袭、转移过程中可能具有协同作用，有望成为抗肿瘤靶向治疗的新靶点。 |
| Other Abstract | Objective To investigate the comparison of enolase-α(ENO1) and tumor M2 pyruvate kinase(M2-PK) expression in gastric cancer and benign gastric tissue, to study the association and clinicopathological significance of ENO1 and M2-PK expression in gastric cancer.
Methods The expression of ENO1 and M2-PK in 55 paraffin-embedded specimens of gastric cancer and 23 paraffin-embedded specimens of benign gastric tissue was detected by SP immunohistochemistry .Specimens of gastric cancer were divided into groups based on parameters such as age,gender,differentiation grade,depth of invasion,lymph node metastasis,TNM staging and so on. The correlation of ENO1 expression to M2-PK expression , and their correlations to the clinicophathologic features of gastric cancer were analyzed.
Results
1．The positive rate of ENO1 was significantly higher in gastric cancer than in benign gastric tissue (67.3% vs . 30.4％, P<0.01) , and closely related to differentiation grade ( P<0.01) , depth of invasion (P<0.01), lymph node metastasis (P<0.05) and TNM staging( P<0.05).
2．The positive rate of M2-PK was significantly higher in gastric cancer than in benign gastric tissue (78.2％ vs . 39.1％, P<0.01) , and closely related to differentiation grade ( P<0.05) , depth of invasion ( P<0.05) .
3． The expression of ENO1 was positively correlated to that of M2-PK (r=0.5729，P<0.05) .
Conclusion The up-regulation of ENO1 and M2-PK expression may play important roles in the oncogenesis and progression of gastric cancer. The ENO1 and M2-PK may take a synergistic effect in the infiltration and the metastasis of the tumor,which should be new targets of molecular targeted therapy. |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/222552
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
倪田根. 胃癌组织中ENO1、M2-PK的表达与临床病理意义[D]. 兰州. 兰州大学,2011.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
