兰州大学机构库 >学院待认领
微核技术在白血病患者遗传损伤研究中的应用价值
Alternative TitleThe application value of Micronucleus technology in genetic damage research of Leukemia patients
王子妍
Thesis Advisor赵丽
2009-05-26
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword微核 细胞周期阻断法 遗传损伤 预后因素
Abstract目的: 应用微核检测结合临床资料分析初诊白血病患者的遗传损伤情况及微核试验结果与预后相关因素间的关系,初步探讨微核试验在白血病发病机制、诊断及预后研究中的价值。 方法 :应用细胞周期阻断法,分析54例初诊白血病患者的骨髓及外周血与30例健康人外周血的微核率、微核细胞率、核芽出现率、核质桥出现率、核分裂指数及细胞凋亡情况,(1)比较初诊白血病患者自身骨髓与外周血微核率及微核细胞率结果,分析其异同;(2)结合染色体中期分析、融合基因检测结果作为染色体损伤指标,与健康人相应指标进行比较,分析初诊白血病患者的遗传损伤;(3)分析完整的31例初诊患者资料,结合患者性别、年龄、初诊白细胞数、血红蛋白数、血小板数目、初诊骨髓中幼稚细胞比例、染色体中期分析结果、融合基因检测结果等临床资料,分析与微核试验结果相关性。 结果 :1. 54例初诊白血病患者外周血微核率(MNR)为11.63±3.05‰,骨髓微核率(MNR)为12.42±1.62‰,经配对t检验,P值均>0.05,差异无统计学意义;外周血微核细胞率(MCR)为10.81±4.92‰;骨髓微核细胞率(MCR)为11.13±3.75‰,经配对t检验,P值均>0.05,差异无统计学意义。2. 54例初诊白血病患者外周血微核细胞率(MCR)为10.81±4.92‰,30例健康人外周血微核细胞率(MCR)为3.81±2.21‰,经t检验,P值<0.01;统计学差异有显著性。初诊白血病患者微核率(MNR)为11.63±3.05‰,健康人微核率(MNR)为3.81±2.21‰,经t检验,P值<0.01;统计学差异有显著性。初诊白血病患者核芽出现率(Buds)为3.50±2.93%,健康人核芽出现率(Buds)为0.20%±0.40,经χ2检验,P值<0.05;有统计学差异。初诊白血病患者核质桥出现率(NPBs)为1.88 ±1.89%,健康人核质桥出现率(NPBs)为0.40 ±0.80%,经χ2检验,P值<0.05;有统计学差异。初诊白血病患者核分裂指数(NDI)为1.51±0.30%,健康人核分裂指数(NDI)为1.22±0.32%,经χ2检验,P值>0.05,差异无统计学意义。初诊白血病患者凋亡细胞出现率(AC)为2.23±1.33%,健康人凋亡细胞出现率(AC)为0.16±0.37%,经χ2检验,P值<0.05;有统计学差异。初诊白血病患者经染色体中期分析异常检出率为24.00%,健康人异常检出率为0.00%,经χ2检验,P值<0.01;统计学差异有显著性。初诊白血病患者融合基因阳性率为18.00%,健康人融合基因检出率为0.00%,经χ2检验,P值<0.01;统计学有显著性差异3.初诊白血病患者性别、年龄、初诊白细胞数、血红蛋白数、血小板数目、初诊骨髓中幼稚细胞比例、染色体中期分析结果、融合基因检测结果与淋巴细胞微核率、微核细胞率、核芽出现率、核质桥出现率、核分裂指数及细胞凋亡情况经相关性检验,微核率、微核细胞率与年龄及染色体异常核型有相关性,核分裂指数与初诊时白细胞数有相关性,其它指标间无明显相关性。 结论 :1.白血病患者的外周血微核细胞率、微核率、核芽出现率、核质桥出现率、凋亡细胞出现率、经染色体中期分析异常检出率、融合基因阳性率均高于健康人相应检测指标,经统计学分析,统计学差异有显著性。白血病患者的外周血核分裂指数略高于健康人,但统计...
Other AbstractObjective :To apply micronucleus test combine clinical data to analyze preliminary diagnosis leukemia patient’s genetic damage, and the relationship between the results of Micronucleus test and prognosis correlation factor. Preliminary investigate the value of micronucleus test in leukemia nosogenesis、diagnosis、prognosis study. Methods : To apply Cytokinesis-blocked micronucleus test analysis 54 preliminary diagnosis leukemia patient’s bone marrow and peripheral blood compare the MNR、MCR、 Buds 、NPBs、NDI、AC with 30 healthy people’s peripheral blood’s results. (1)compare the MNR、MCR between the leukemia patient’s bone marrow and peripheral blood ,analysis the different ;(2)combined the chromosome metaphase analysis and gene detection as the target of the chromosome damage, compare with healthy people’s ,to analysis the leukemia patient’s inheritance damage;(3)select 31 complete case, combined the sex ,age ,the count of the white blood cell ,the count of the hemoglobin ,the count of the platelet ,the proportion of the naïve cell in bone marrow ,chromosome metaphase analysis result ,gene detection ,analysis the correlation with the result of the micronucleus test. Results : 1.The 54 preliminary diagnosis leukemia patient’s peripheral blood’s MNR is 11.63±3.05‰, MCR is 10.81±4.92‰;bone marrow ‘s MNR is 12.42±1.62‰,MCR is 11.13±3.75‰, though the t test, P value >0.05, no statistical significance differences.2. The 54 preliminary diagnosis leukemia patient’s peripheral blood’s MCR is 10.81±4.92‰,30 healthy people’s peripheral blood’s MCR is 3.81±2.21‰, though the t test, P value<0.01, existence statistical significance differences.The leukemia patient’s peripheral blood’s MNR is 11.63±3.05‰,healthy people’s peripheral blood’s MNR is 3.81±2.21‰,though the t test, P value<0.01, existence statistical significance differences. The leukemia patient’s peripheral blood’s Buds 3.50±2.93%,healthy people’s peripheral blood’s Buds 0.20%±0.40,though the χ2 test, P value<0.05;existence statistical significance differences. The leukemia patient’s peripheral blood’s NPBs is 1.88 ±1.89%,healthy people’s peripheral blood’s NPBs is 0.40 ±0.80%,though the χ2 test, P value<0.05;existence statistical significance differences. The leukemia patient’s peripheral blood’s NDI is 1.51±0.30%,healthy people’s peripheral blood’s NDI is 1.22±0.32%,though the χ2 test,,P value >0.05,no statistical significa...
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222596
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
王子妍. 微核技术在白血病患者遗传损伤研究中的应用价值[D]. 兰州. 兰州大学,2009.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Altmetrics Score
Google Scholar
Similar articles in Google Scholar
[王子妍]'s Articles
Baidu academic
Similar articles in Baidu academic
[王子妍]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[王子妍]'s Articles
Terms of Use
No data!
Social Bookmark/Share
No comment.
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.