|Alternative Title||Effect of Pseudomonas Aeruginosa on murine splenic cells and development of bladder cancer model in mice
|Place of Conferral||兰州
|Other Abstract|| Objective: To explore the optimization for establishment of mouse models of orthotopic bladder tumors, to evaluate the effects of pseudomonas aeruginosa (PA-MSHA) on bladder cancer in mouse subcutaneous tumor model and on the proliferation of splenic lymphocytes, to explore the mechanism involved in, and to provide new treatment strategies for bladder cancer.
Methods: After 1 or 24 hours the pretreatment of murine bladder mucosa with silver nitrate, HCl, trypsin and ethanol, the mice were killed to obtain bladder tissue samples. Pathological changes were observed using hematoxylin and eosin staining. The microenvironment modification was assessed by electron microscopy. Mast cells were counted with toluidine blue staining. The glycosaminoglycan (GAG) layer was observed by periodic acid-Schiff staining. Mice were intravesically pretreated with the above four reagents, respectively, and the rate of tumor formation was determined. Subcutaneous bladder tumor model was established, and divided into four groups: normal saline treatment group, low PA-MSHA dose treatment group, medium PA-MSHA dose treatment group, and high PA-MSHA dose treatment group. PA-MSHA or normal saline were injected around the tumors. Three mice were sacrificed in each group at 14, 21 and 28 after implantation, serum was collected from each mice at 3000×g for 5 minutes, then CD4+ or CD8+ were detected through flow cytometry analysis. Tumor tissue, and major organs were obtained to observe pathological changes, VEGF and MVD were also assessed. The spleens were removed aseptically from C57BL/6 mice, then spleen cell suspensions were treated with low PA-MSHA dose, medium PA-MSHA dose, high PA-MSHA dose and RPMI-1640 culture medium as control for 24, 48, 72 h. At the end point, the optical density (OD) values of the samples were measured on a microplate reader.
Results: In the model mice, a part of umbrella cells were sloughed off, the submucosal layer was exposed and incontinuous in some areas at one hour after pretreatment with trypsin and ethanol. Mucosal damages were more serious in the HCl and silver nitrate groups. However, there was no significant difference in the inflammatory cell infiltration between the experiment and control groups. Mild edema and congestion in the mucosa, tightly arranged epithelial cells, and continuous mucosa were observed in the mice after trypsin and ethanol pretreatment for 24 hours.
By contrast, the thickness of mucosa was uneven ...|
李涛. 铜绿假单胞菌制剂对小鼠膀胱癌和脾细胞作用的机制研究[D]. 兰州. 兰州大学,2014.
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