| 糖尿病大鼠胃动力变化与胃血管变化关系的研究 |
| Alternative Title | To investigate the correlative of the gastric motility change and gastric vasal change in type 2 diabetic rats
|
| 张喜娟 |
| Thesis Advisor | 严祥
; 刘永铭
|
| 2010-05-26
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 胃动力
血管
2型糖尿病
大鼠
内皮细胞
|
| Abstract | 糖尿病大鼠胃动力变化与胃血管变化关系的研究
目的：研究糖尿病胃动力变化与胃血管变化的关系。
方法：72只SPF级雄性Wistar大鼠适应性喂养一周，随机分为实验组和对照组，实验组动物采用高脂高糖饲料喂养[1]诱导并小剂量链脲佐菌素（STZ）注射破坏胰岛β细胞，制造2型糖尿病模型。分别于4周，24周时应用单光子发射断层显像仪（SPECT）技术测定胃排空，观察血内皮素（ET-1）、血栓调节蛋白(TM)、血管性假性血友病因子 (vWF)，并在显微镜下观察胃血管的病理变化。
结果：24周模型组大鼠血浆TM、vWF、ET-1显著升高，4周模型组轻度升高。显微镜观察见早期血管内皮轻度损害，微血管代偿性增加，胃动力加快。随着病程进展，血管内皮损害进一步加重，胃粘膜微血管逐渐减少，毛细血管扩张，粘膜下及肌层小血管管壁增厚，管腔狭窄，管壁/管腔比值明显增大，胃动力延缓。
结论：糖尿病胃动力变化与胃血管变化相关，并且血管病变可能是引起胃动力变化的始动因素。 |
| Other Abstract | To investigate the correlative of the gastric motility change and gastric vasal change in type 2 diabetic rats
Objective:To investigate the correlative of the gastric motility change and gastric vasal change in type 2 diabetic rats.Methods:72 male SPF Wistar rats were first divided random into two groups: control (n=24) and diabetic group (n=48). The control was fed ordinary laboratory diet for 20 weeks, but the diabe- tic group was fed fat- and caloric-rich laboratory diet for 6 weeks and then inject- ed with small dose of streptozotocin(30mg/kg), until 24weeks to develop diabet- ic gastric motility disorders model. Gastric emptying was measured by SPECT after 4,24weeks either. In addition observing plasma endothelin (ET-1), thromb- omodulin (TM), vWillebrand factor (vWF), and observed under a microscope, the gastric peripheral vascular and microvascular pathological changes.Results: The plasma TM, vWF, ET-1 increased significantly in 24 weeks and increased slightly in 4 weeks. Early microvascular compensatory increase，and gastric motility speed up. As the illness progresses, gastric microvascular diminishing、telangiectasia、small vessel wall thickening in submucosal and muscular layers, stenosis,wall / lumen ratio significantly increased.Conclusion: The vascular changes associated with changes in gastric motility in diabetes. |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/222659
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
张喜娟. 糖尿病大鼠胃动力变化与胃血管变化关系的研究[D]. 兰州. 兰州大学,2010.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
