| 使用多重PCR技术快速检测VAT和VAP患者常见病原菌的研究 |
| Alternative Title | Study of common pathogenic Bacteria of rapid detection with multiplex PCR for the patients with VAT and VAP
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| 王小红 |
| Thesis Advisor | 董晨明
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| 2012-05-22
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 多重PCR
呼吸机相关性气管支气管炎
呼吸机相关性肺炎
病原体
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| Abstract | 目的 评价多重PCR在快速诊断呼吸机相关性气管-支气管炎和肺炎常见病原菌中的临床作用和价值。方法 选择常见病原体特异性基因序列，即金黄色葡萄球菌的mecA、FemA基因、铜绿假单胞菌的脂蛋白I基因（OprI）、大肠埃希氏杆菌的PhoA基因、肺炎克雷白杆菌的mdh基因以及鲍曼不动杆菌的OXA-51型酶基因（blaOXA-51-like）设计并合成6对引物，通过经典酚氯仿提取痰液标本中的DNA，扩增病原体DNA后电泳并观察其结果，同时给予普通PCR和分离培养作为对照。结果 (1) 多重PCR与普通PCR检测比较:对痰标本同时进行多重PCR和普通PCR检测，两者均检测出金黄色葡萄球菌20例，鲍曼不动杆菌20例，大肠埃希氏杆菌10例，绿脓假单胞菌20例，肺炎克雷白杆菌15例，其中有15份标本为以上任何2种菌的混合感染。多重PCR与普通PCR的符合率达100％。结论 M-PCR技术能够高通量、快速检测诊断病原体，具有较好的敏感性和特异性。可能为VAT或VAP的临床诊断提供一个新的快速检测手段，也为临床治疗提供更为准确的参考依据。 |
| Other Abstract | Objective To observe the rapid diagnosis value of multiplex PCR in patients with Ventilator-Associated Tracheobronchitis and Ventilator-Associated Pneumonia.Methods Select a common pathogen-specific gene sequences, Staphylococcus aureus mecA, femA genes、Pseudomonas aeruginosa lipoprotein I gene (OprI)、Escherichia coli PhoA genes、Kpneumoniae mdh genes、Acinetobacter baumannii OXA-51 type enzyme gene, Through the DNA in the sputum of classic phenol-chloroform extraction, the amplified pathogen DNA electrophoresis and observe the results, while giving the PCR and separation of culture as a control. Conclusion M-PCR is a high- flux, rapid detection and diagnosis of pathogens, with better sensitivity and specificity。May provide a new means of rapid detection of VAT or VAP clinical diagnosis, provide a more accurate reference for clinical treatment. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222700
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
王小红. 使用多重PCR技术快速检测VAT和VAP患者常见病原菌的研究[D]. 兰州. 兰州大学,2012.
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