| 肾癌肿瘤干细胞CD73和DLL4表达情况研究及其重组PEAs-pVAX1-DLL4转染移植瘤鼠的实验研究 |
| Alternative Title | The Study on the expression of CD73、DLL4 in RCC and the research of recombinant PEAs-pVAX1-DLL4 in the subcutaneously transplanted tumor in rat
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| 于忆 |
| Thesis Advisor | 岳中瑾
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| 2015-11-29
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 博士
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| Keyword | CD73
P糖蛋白
Delta样分子4
疫苗
重组质粒PEAs-pVAX1-DLL4
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| Abstract | 159例肾癌标本通过免疫组化技术（二步法）对表面抗原CD73及DLL4，P-gp表达判定。CD73 在159例肾细胞癌中75例表达增强，存在统计学意义（p = 0.000）；P-gp 的染色在85例肾癌组织中有42例在血管内呈强表达并存在统计意义(p = 0.000)。DLL4在肾癌组织中（52/89例；58.43%）表达比较有统计学意义(p = 0.000)。CD73 在肾癌细胞中的阳性表达水平与肿瘤类型（p = 0.000 ）、肿瘤TNM分期(p = 0.002)、肿瘤分级(p = 0.002)存在显著相关性。P-gp 在RCC的阳性表达仅与肾细胞癌TNM分期(p = 0.015)和肿瘤分级(p = 0.026)存在统计学意义。DLL4与RCC肿瘤TNM分期存在统计学差异(p = 0.009)。通过Cox回归模型进行生存分析和风险比例评估，CD73(p = 0.037) 和肿瘤分级(p = 0.035)与患者生存期相关；CD73强表达的患者中位生存期为62.06 5.35 月, 明显短于CD73 表达欠缺的103.72 3.67 月。故而得出，CD73的表达水平与肾细胞癌密切相关。
检测质粒重组体PEAs-pVAX1-DLL4转染皮下移植瘤鼠对肿瘤抑制的效果探讨。DLL4质粒组的昆明鼠生存时间为33.14±6.06天，对照组为27.67±6.02天。瘤体对照组（17.11±9.30 cm3）与质粒组体积（5.08±2.75cm3）间存在统计学差异(P=0.035)。质粒组肿瘤生长抑制率为70.31%，瘤重抑制率34.19%。CD4阳性表达在质粒组（32.41±4.19 pg/ml）显著性增高，与对照组15.67±7.02 pg/ml（P =0.001）、正常组17.99±6.71 pg/ml（P =0.005）存在差异。IL2，IL4，r-IFN在质粒组表达最高，存在统计学意义。DLL4 CDNA可在体外、体内进行转染，延缓肿瘤生长。 |
| Other Abstract | The specimens of 159 cases of renal cancer were tested the expression proteins of CD73 , DLL4 and P - gp by immunohistochemical technique (two-step method) . In comparison with the normal tissue, CD73 express on 75 cases in 159 cases of renal cell carcinoma（p = 0.000) . Strong expression of P - gp are 42 cases of 85 cases of kidney tissue within the blood vessels and there is statistical significance. 58.43% DLL4 expression (52/89 cases) comparing 5.89% normal tissue (1/17cases) is statistically significant (p = 0.000). CD73 expression level in kidney cells and tumor type（p = 0.000 ）, tumor TNM staging (p = 0.002)and grading (p = 0.002)exist significant correlation. P - gp is only related to renal cell carcinoma TNM staging(p = 0.015)and grading(p = 0.026). DLL4 expression level is significant correlation with tumor TNM staging (p = 0.009).COX multivariate linear regression analysis, only the expression of CD73 (p = 0.037) and tumor grade (p = 0.035) associated with patient survival in RCC. In the kidney cancer cells, the median survival time of the CD73 patients with strong expression (62.06 + / - 5.35 months)was significantly shorter than the lack expression of CD73 (103.72 + / - 103.72 months). CD73 expression level is closely related to renal cell carcinoma。.
Transplantation tumor were injected recombinant plasmids PEAs – pVAX1 - DLL4 exploried the level of molecular biology and effect. DLL4 plasmid group of kunming mice survival time is 33.14±6.06 days, comparing control group survival 27.67±6.02 days. Tumors volume of control group（17.11±9.30 cm3）has statistical significance(P=0.035) comparing with plasmid group （5.08±2.75cm3）.Plasmid tumor growth inhibition rate was 70.31%, wheareas tumor inhibition rate was 34.19%. CD4 positive expression increased significantly on plasmid group （32.41±4.19 pg/ml）compared with the control group15.67±7.02 pg/ml（P =0.001） , the empty plasmid group 17.99±6.71 pg/ml（P =0.005）. The expression of IL2, IL4, r - IFN is highest in plasmid groups, significancly. So, DLL4 CDNA may transfect in vitro and in vivo and inhabited tumor growth . |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222727
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
于忆. 肾癌肿瘤干细胞CD73和DLL4表达情况研究及其重组PEAs-pVAX1-DLL4转染移植瘤鼠的实验研究[D]. 兰州. 兰州大学,2015.
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