| 舍曲林联合氟康唑抗白念珠菌生物膜活性的体外研究 |
| Alternative Title | Antifungal Activity of Sertraline in Combination with Fluconazole against Candida albicans Biofilm in vitro
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| 吴真珍 |
| Thesis Advisor | 骆志成
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| 2015-05-19
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 白念珠菌
氟康唑
舍曲林
生物膜
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| Abstract | 近年来随着器官移植等的增多致白念珠菌感染最为常见。通常这些感染与生物膜的形成有关。目前临床上安全且行之有效的抗真菌药物数量较少，这中包括氟康唑。目的：探讨在体外舍曲林对白念珠菌是否有抑制作用；评价两药之间有无联合抗真菌作用。方法：（1）在体外测定氟康唑和舍曲林的最小抑菌浓度（MIC）。（2）测定两药之间有无相加作用。结果：（1）氟康唑对Ⅰ、Ⅱ的MIC值为8μg/ml,8μg/ml，对Ⅲ的MIC值为128μg/ml。（2）舍曲林对临床菌株Ⅰ、Ⅱ及Ⅲ生物膜的MIC50值分别为475μg/ml、237.5μg/ml及＞475μg/ml，氟康唑对临床菌株Ⅰ、Ⅱ、Ⅲ的MIC50值均＞128μg/ml；联合用药时氟康唑对临床菌株Ⅲ无增强作用，对临床株Ⅰ、Ⅱ的表现为相加作用。结论：（1）舍曲林对白念珠菌表现出一定的抑制作用。（2）白念珠菌形成生物膜后对氟康唑表现出耐药性。（3）舍曲林可能通过抑制菌丝的生长而增加氟康唑对生物膜的敏感性。 |
| Other Abstract | Background：In recent years, The incidence of fungal infections are become increasingly because of organ transplantation. Candida albicans is implicated in many biomaterial-related infections. The number of effective and safe antifungal drugs is limited. including fluconazole.
Objective: To determine the antiCandida Albicans activity of sertraline in vitro; To assess combination against Candida albicans biofilm in vitro.
Method: (1) The minimus inhibitory concentration(MIC) of sertraline, fluconazole to clinical strains. (2)To evaluate the combined effect of these two drugs.
Result: (1) The MIC50 of fluconazole for Ⅰ, Ⅱ and Ⅲ were 8μg/ml, 8μg/ml and 128μg/ml; (2) The MIC50 of sertraline for Ⅰ, Ⅱ and Ⅲ were 475μg/ml, 237.5μg/ml and ＞475μg/ml, the MIC50 of fluconazole for biofilms cells of strains were ＞128μg/ml.
Conclusion: (1) Sertraline has antifungal effects on Candida albicans. (2) Candida albicnas biofilm was resistant to fluconazole; (3) sertraline may increase the sensitivity of biofilm cells of Candida albicans to fluconazole by devoid of hyphal growth.
Keywords：Candida albicans, Fluconazole, Sertraline, Biofilm |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222748
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
吴真珍. 舍曲林联合氟康唑抗白念珠菌生物膜活性的体外研究[D]. 兰州. 兰州大学,2015.
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