| 三七总皂苷对大鼠肾脏损伤保护作用的实验研究 |
| Alternative Title | An experimental study on protection of panax notoginseng saponins after renal injury of rats
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| 陈丑彦 |
| Thesis Advisor | 孙少华
; 冯颖
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| 2011-05-19
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 三七总皂苷
肾脏
创伤
骨折
细胞凋亡
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| Abstract | 目的：研究三七总皂苷对肾脏损伤后细胞凋亡及凋亡调控基因的影响，探讨三七总皂苷对肾脏损伤的保护机制。
方法：大鼠经轻度腹腔麻醉后，利用自由落体式生物撞击仪直接打击大鼠脊肋区制作肾脏创伤模型，打击股骨干中段制作骨折模型。实验动物分为五组，单纯创伤组（36只）、单纯骨折组（36只）、创伤给药组（36只）、骨折给药组（36只）、正常对照组（6只），前四组再平均分为6组，分别在打击后1、6、12、24、36、48小时各处死6只，给药组于创伤后即刻予以腹腔注射三七总皂苷（70mg/kg），骨折组，创伤组及对照组给予腹腔注射同等剂量的生理盐水。实验动物颈椎脱臼法处死后，迅速解剖动物，提取肾脏标本，中性福尔马林及4%多聚甲醛固定。HE染色观察创伤及骨折后肾脏组织的基本病理改变。采用免疫组化技术及原位杂交技术检测Bcl-2、Bax在肾脏组织中的表达规律，利用TUNEL技术观察凋亡细胞的变化及分布的规律性。 |
| Other Abstract | Objective To study the effect of panax notoginseng saponins(PNS) on the apoptosis, apoptosis-related gene expression in the renal tissue, and to explored the regulation mechanism of PNS protection..
Methods The model of renal trauma and femoral fracture were established through the free-fall biological impact instrument against the spinal rib area and the middle femur of rats. Westar rats were randomly divided into five groups: normal control(n=6), renal trauma(n=36), trauma treatment (n=36), fracture(n=36), and fracture treatment (n= 36). The injured rats were executed at 1, 6, 12, 24, 36, and 48 hours after injury (n=6 per time point). In the treatment group, each rat was injected with PNS through intraperitoneal (70mg/kg). Renal specimens were rapidly collected, fixed in 4% neutral formalin and paraformaldehyde, embed in paraffin, made tissue chips for observing the histological change with HE staining, the expression of Bcl-2/Bax and the transcriptions of Bcl-2 mRNA /Bax mRNA by immunohistochemistry and situ hybridization. The apoptosis was examined using TUNEL staining skill. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222771
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
陈丑彦. 三七总皂苷对大鼠肾脏损伤保护作用的实验研究[D]. 兰州. 兰州大学,2011.
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