兰州大学机构库 >学院待认领
脑源性神经营养因子与2型糖尿病患者颈动脉粥样硬化的关系
Alternative TitleRelationship of brain derived neurotrophic factor and carotid atherosclerosis in type 2 diabetes mellitus
邱世超
Thesis Advisor任建功
2014-05-23
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword脑源性神经营养因子 2型糖尿病 颈动脉粥样硬化 内皮细胞生长因子
Abstract目的: 通过研究2型糖尿病(T2DM)伴发颈动脉粥样硬化患者(C组,64例)、T2DM非颈动脉粥样硬化患者(B组,56例)和健康人群(A组,60例)血清脑源性神经营养因子(BDNF)水平、血管内皮生长因子(VEGF)及外周血淋巴细胞BDNF mRNA表达水平,探讨BDNF与T2MD颈动脉粥样硬化的关系及其可能机制。 方法: 收集兰州大学第二医院糖尿病2科住院T2DM患者120例,依据颈动脉彩色多普勒超声显像分为伴发颈动脉粥样硬化组64例(C组)和非颈动脉粥样硬化组56例(B组)。同时收集我院同一时间体检中心健康体查者60例作为本实验健康对照组(A组)。测定计算3组年龄、性别、糖尿病病程(Dd)、血脂、血压、空腹血浆葡萄糖(FPG)、空腹胰岛素(FINS)、糖化血红蛋白(HbA1c)、颈动脉内中膜层厚度(CIMT)、BDNF、外周血淋巴细胞BDNF mRNA表达水平、血清血管内皮生长因子(VEGF),计算稳态胰岛素抵抗指数。2组间均数比较采用t检验,3组间均数比较采用单因素方差分析,BDNF相关分析采用Pearson相关分析和逐步回归分析。 结果 (1) T2DM组血清BDNF水平(6.21±3.31ng/ml)及BDNF mRNA表达水平(4.42±1.23)显著低于健康对照组(9.97±2.59 ng/ml,5.86±1.29,均P<0.05)。T2DM组患者血清VEGF水平(66.65±21.64 pg/ml)明显高于健康对照组(49.29±20.23 pg/ml ,P<0.05)。(2)C组BDNF(5.62±3.41ng/ml )及BDNF mRNA(4.10±1.13)明显低于B组(6.88±3.10 ng/ml, 4.77±1.25,P<0.05)及A组 (9.97±2.59 ng/ml,5.86±1.29 ,P<0.05);C组 VEGF(71.34±20.30 pg/ml)明显高于B组(61.29±22.05 pg/ml,P<0.05)及A组(49.29±20.23 pg/ml, P<0.05)。(3)Pearson相关分析显示,C组血清BDNF与CIMT、BDNF mRNA、VEGF呈相关关系,(r值分别为-0.221,0.359,-0.372,均P<0.05)。(4)多元逐步回归分析表明,C组CIMT与血清BDNF具有直线回归关系(r2值为0.157,P<0.05)。 结论:T2DM合并颈动脉粥样硬化患者血清BDNF水平、BDNFmRNA水平降低、VEGF水平升高,提示血清低浓度BDNF及高浓度VEGF共同参与颈动脉粥样硬化的发生及发展。
Other AbstractObjective: To investigate the relationship between brain-derived neurotrophic factor (BDNF) and carotid atherosclerosis in patients with type 2 diabetes mellitus(T2DM) through detecting the serum BDNF level , serum vascular endothelial growth factor(VEGF) level and the expression of BDNF mRNA in peripheral blood lymphocytes in patients with carotid atherosclerosis(group C ,n=64), patients without carotid atherosclerosis(group B,n=56) and healthy individuals(group A,n=56). Methods: A total of 120 T2DM patients were assigned to non carotid atherosclerosis group( group B, n=56) and the carotid atherosclerosis group( group C,n=64). 60 healthy controls were recruited from health check-up populations of Lanzhou university second hospital (group A,n=60). Age,Sex, diabetes duration(Dd), lipids, blood pressure, fasting plasma glucose(FPG), serum insulin, hemoglobin A1C, carotid intima-media thickness(CIMT), serum BDNF, BDNF mRNA, VEGF, and insulin resistance were measured. Student’s t test and analysis of variance were used for data analysis. Results: (1)Both serum level of BDNF(6.21±3.31ng/ml) and the expression of BDNF mRNA (4.42±1.23)in peripheral blood lymphocytes in T2DM patients were significantly lower than that in group A(9.97±2.59 ng/ml, 5.86±1.29, P<0.05). The serum level of VEGF (66.65±21.64 pg/ml)in T2DM patients was significantly higher than that in group A(49.29±20.23 pg/ml ,P<0.05). (2) Both serum level of BDNF(5.62±3.41 ng/ml) and the expression of BDNF mRNA(4.10±1.13) in peripheral blood lymphocytes in group C were significantly lower than that in group A (9.97±2.59 ng/ml,5.86±1.29, P<0.05)and group B(6.88±3.10 ng/ml, 4.77±1.25,P<0.05). The serum level of VEGF in group C (71.34±20.30 pg/ml)was significantly higher than that in group A(49.29±20.23 pg/ml, P<0.05)and group B(61.29±22.05 pg/ml,P<0.05).(3)In pearson correlation analysis, serum BDNF was correlated with CIMT, BDNF mRNA and VEGF in group C (r value were -0.221, 0.359, -0.372, respectively ; all P<0.05).(4)Multiple stepwise regression analysis showed that serum BDNF was the independent variable of CIMT in group C(r2 value was 0.157, P<0.05). Conclusions:The level of serum BDNF and BDNFmRNA are significantly decreased and the VEGF is significantly increased in T2DM with carotid atherosclerosis, suggesting that low level of BDNF and high level of VEGF are correlated with the occurrence and development of carotid atherosclerosis.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/222948
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
邱世超. 脑源性神经营养因子与2型糖尿病患者颈动脉粥样硬化的关系[D]. 兰州. 兰州大学,2014.
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