| 脑钠素T-381C基因多态性与原发性高血压遗传易感性的研究 |
| Alternative Title | Relationship study between T-381C gene polymorphism of brain natriuretic peptide and genetic susceptibility to essential hypertension
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| 周振磊 |
| Thesis Advisor | 马丽雅
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| 2009-04-30
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 原发性高血压
脑钠素
基因
多态性
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| Abstract | 背景与目的: 脑钠素(brain natriuretic peptide, BNP)基因启动子区域-381bp处存在T/C多态性位点，国外研究表明此位点与血浆BNP水平有关，但BNP基因型与原发性高血压的易感性关系尚未见报道，本研究拟对此问题进行探讨。
方法: 高血压组138例，对照组141例。两组年龄、体重指数差异无统计学意义。对两组进行血压测量，空腹12小时以上采血检测血糖、总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇等。聚合酶链式反应-限制性片段长度多态性方法进行DNA多态性分析，琼脂糖凝胶电泳判断基因型。
结果：检测到TT、TC、CC三种基因型。在高血压病组与对照组之间基因型构成比差异有统计学意义（P <0.05）。在高血压组TC-CC基因型和C等位基因频率（0.034和0.178）显著高于对照组（分别为0.199和0.106），P值分别为0.042和0.016。在高血压组内TT基因型的空腹血糖高于TC-CC基因型，差异有统计学意义（P=0.045）。
结论：脑钠素T-381C基因多态性与原发性高血压遗传易感性相关，TC-CC基因型和C等位基因发生高血压的风险较大。
关键词 原发性高血压；脑钠素；基因；多态性； |
| Other Abstract | OBJECTIVE: A -381 bp T/C polymorphism located in the promoter region of brain natriuretic peptide（BNP） gene was found to be associated with plasma concentration of BNP. However，its association with essential hypertension remained unclear .The aim of this study was designed to explore this association in Chinese population.
METHODS: 138 essential hypertensive patient (male 87 and female 51,age 49.28 ± 11.19 y,and body mass index 24.12±1.96kg/ m2<上标!>) and 141 normal controls (male 95 and female 46,age 48.17±12.66 y,and body mass index23.94±2.42kg/m2<上标!>)were genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Phenotypic measure- ments include blood pressure,blood glucose,serum triglyceides,serum total high-density lipoprotein (HDL) and low- density lipoprotein (LDL).
RESULTS: The frequencies of TC and CC genotypes (0.304) and C allele (0.178) were significantly (P=0.042 and 0.016) higher in hypertensive patients than normal controls (0.199 and 0.106). Furthermore , C allele carriers , compared with TT subject , had lower concentrations of blood glucose in hypertensive patients (P=0.045) .
CONCLUSION: The brain natriuretic peptide T-381C polymorphism might correlate with genetic susceptibility to essential hypertension in Chinese .The risk of essential hypertension was increased in the persons carrying allele C or genotype TC and CC. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/222954
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
周振磊. 脑钠素T-381C基因多态性与原发性高血压遗传易感性的研究[D]. 兰州. 兰州大学,2009.
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