| 淋巴毒素αNcoI基因多态性与冠心病遗传易感 |
| Alternative Title | Association of lymphotoxin α NcoI gene polymor- -phism and the genetic predisposition of coronary heart disease
|
| 高涵翔 |
| Thesis Advisor | 张钲
; 白锋
|
| 2008-05-06
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 冠心病
淋巴毒素α
基因多态性
遗传易感性
|
| Abstract | 目的：检测淋巴毒素α（LT-α）NcoⅠ基因多态性，并探讨其与我国汉族冠心病（CHD）遗传易感性之间的关系。
方法：选取家族史阳性的汉族冠心病患者57例，家族史阴性的汉族正常对照62例，进行血压测量，采集静脉血，检测甘油三酯（TG），总胆固醇（TC），高密度脂蛋白胆固醇（HDL-C），低密度脂蛋白胆固醇（LDL-C）等，采集动脉血，提取DNA，以聚合酶链反应-限制性片断长度多态分析（PCR-RFLP）的方法，进行DNA多态性的检测，以分析LT-α基因型以及基因频率分布。
结果：(1)LT-α NcoⅠ基因多态性含有两个等位基因(LT-α1和LT-α2) 及三种基因型(纯合子LT-α1/1和LT-α2/2 ,杂合LT-α1/2)；(2)冠心病组的LT-α的基因型与正常对照组差异无显著性（χ2 = 1.479 , P>0.05）；(3)LT-α的基因型与冠状动脉狭窄程度差异无显著性（χ2 = 6.495, P >0.05）。 |
| Other Abstract | Objective: To identify polymorphism of lymphotoxin α (LT-α), and to determine whether lymphotoxin α (LT-α) NcoI gene polymorphism is associated with genetic predisposition of coronary heart disease in Chinese Han population.
Methods: 57 coronary atherosclerotics with family history of coronary heart disease and 62 healthy controls were included in the study. We analysed all the samples′ allele frequency and genotype frequency of LT-α by PCR-RFLP. Measurement included blood pressure, serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C).
Results: LT-α Ala252Gly gene polymorphism had 2 alleles(LT-α1 and LT-α2) and 3 kinds of genotype: homozygote LT-α 1/1, LT-α 2/2 and heterozygote LT-α 1/2; No population significant differences were detected in LT-α genotypes and allele frequencies between coronary heart disease patients or healthy controls（χ2 = 1.479 , P = 0. 477>0.05）. The gene polymorphism was not associated with the degree of coronary artery stenosis as well（χ2 = 6.495, P = 0. 592>0.05）.
Conclusion: LT-α Ala252Gly gene polymorphism was not associated with the genetic predisposition of coronary heart disease. The allele frequency and genotype frequency of LT-α was not correlated with the degree of coronary artery stenosis as well. |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/223053
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
高涵翔. 淋巴毒素αNcoI基因多态性与冠心病遗传易感[D]. 兰州. 兰州大学,2008.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
