兰州大学机构库 >学院待认领
粒细胞集落刺激因子对大鼠脊髓损伤后运动功能的影响
Alternative TitleGranulocyte-Colony Stimulating Factor improves motor function recovery after spinal cord injury in rats
李昭成
Thesis Advisor王文己
2013-05-27
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword粒细胞集落刺激因子 脊髓损伤 运动功能 治疗 恢复
Abstract背景:粒细胞集落刺激因子是一种可以刺激细胞分化、增殖、存活的粒细胞系中的蛋白质。近期,有文献报道了粒细胞集落刺激因子在脑梗塞模型及急性脊髓损伤模型中的神经保护作用。目的:通过观察粒细胞集落刺激因子治疗脊髓损伤大鼠模型的运动功能恢复情况,探讨其治疗脊髓损伤的可能性。方法:50只成年Wistar大鼠,4只仅做椎扳切除术,其余46只使用改良的Allen’s法制作T10节段脊髓损伤撞击模型后随机分为两组: vehicle组(n=23)和G-CSF组(n=23),G-CSF组应用粒细胞集落刺激因子治疗,vehicle组注射等剂量磷酸盐缓冲液。于术后1、7、14、21、28、35天分别应用BBB运动功能评分法和Rivlin斜板实验及术后7、14、21、28、35天使用网格步行试验,对两组动物的运动功能恢复情况进行行为学评估。结果:术后G-CSF组3只、vehicle组4只动物因创伤、感染等因素死亡,其余动物均存活至实验观察结束。所有动物术后均出现后肢瘫痪症状。与vehicle组相比,G-CSF组术后的BBB运动功能评分第7天p<0.05,第14、21、28、35d天p<0.01;网格步行试验的错误数第14天p<0.05,第21、28、35天p<0.01;Rivlin斜板实验评分第7、14、21、28、35天p<0.01,各项行为学评分均有显著差异。通过统计学处理发现,G-CSF组各项评分均优于vehicle组。结论:粒细胞集落刺激因子对脊髓损伤模型运动功能的恢复有较好的效果。
Other AbstractBackground: Granulocyte colony-stimulating factor (G-CSF) is a protein that stimulates differentiation, proliferation, and survival of cells in the granulocytic lineage. Recently, a neuro protective effect of G-CSF was reported in a model of cerebral infarction and a model of spinal cord injury (SCI). Aim: To investigate the possibility of Granulocyte-Colony Stimulating(G-CSF) treat spinal cord injury therapy by observing beneficial effects of G-CSF on motor function recovery after spinal cord injury in rats. Methods: 50 adult Wistar rats, 4 of them received only the laminectomy but no impact (n= 4). The rest 46 animals were subjected to the spinal cord injury model at T10 section using improved Allen’s weight drop method. Randomly divided the rest 46 animals into vehicle group (n=23) and G-CSF group (n=23), G-CSF group were treated post-operatively with G-CSF, and vehicle group use PBS. The animals’ motor behavior was evaluated with Basso-Beattie-Bresnahan score (BBB score)and modified Rivlin loxotic plate test monitored weekly for 5 weeks postinjury (day 1, 7, 14, 21, 28 and 35), and Grid walk test from 2nd week for 4 weeks post-operatively(day 7, 14, 21, 28 and 35). Results: Seven animals died before the observation period for trauma or infection, three in G-CSF group and four in vehicle group, the rest were survived till the observation ended. Hind limbs paralysis occurred in all animals postoperatively. There are significant differences between two groups with a positive influence of G-CSF treatment on the motor behavior test(p<0.05 at day 7 and p<0.01 at day 14, 21, 28, 35 of mean BBB score. p<0.05 at day 14 and p<0.01 at day 21, 28, 35 of Mean Grid walk test errors. p<0.01 at day7, 14, 21, 28and 35 of Mean modified Rivlin loxotic plate degree). Conclusion: Consulted all SCI studies which report improved functional recovery following G-CSF therapy, and together with the results of this experiment, G-CSF is play a positive influence of treatment on the motor functional recovery.
URL查看原文
Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223068
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
李昭成. 粒细胞集落刺激因子对大鼠脊髓损伤后运动功能的影响[D]. 兰州. 兰州大学,2013.
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