兰州大学机构库 >学院待认领
利多卡因复合氯胺酮对全脑缺血大鼠海马细胞凋亡的影响
Alternative TitleEffects of lidocaine combined ketamine on apoptosis in hippocampus following global cerebral ischemic injury in rats
张全云
Thesis Advisor石翊飒
2009-05-18
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword利多卡因 氯胺酮 脑缺血/再灌注 海马 细胞凋亡
Abstract目的:研究利多卡因复合氯胺酮对全脑缺血/再灌注大鼠海马CA1区细胞坏死和凋亡的影响。 方法:Wistar大鼠60只,随机分为6组:对照组(Ⅰ组n =4)、假手术组(Ⅱ组n =4)、模型组(Ⅲ组n =4)、利多卡因组(Ⅳ组n =16)、氯胺酮组(Ⅴ组n =16)、利多卡因复合氯胺酮组(Ⅵ组n =16),采用四血管阻断法制备全脑缺血/再灌注模型。Ⅳ、Ⅴ、Ⅵ组在夹闭血管前15min分别腹腔注射利多卡因10mg/kg;氯胺酮10mg/kg;利多卡因复合氯胺酮混合液10mg/kg,再灌注12、24、48、72h行HE染色和细胞凋亡(TUNEL法)检测,观察大鼠海马CA1区细胞坏死和凋亡。 结果:与Ⅱ组比较,Ⅳ、Ⅴ、Ⅵ组24 h缺血神经元显著增多,差异有统计学意义(P<0.05 );Ⅵ组缺血神经元较Ⅳ、Ⅴ组显著减少,差异有统计学意义(P<0.05),Ⅳ、Ⅴ组间无统计学差异,缺血神经元高峰在24h出现。与Ⅱ组相比,Ⅳ、Ⅴ、Ⅵ组24h凋亡细胞显著增多,差异有统计学意义(P<0.05 ),Ⅵ组细胞凋亡数较Ⅳ、Ⅴ组减少,差异有统计学意义(P<0.05 );Ⅳ、Ⅴ组间无统计学差异,细胞凋亡高峰在24h到48h间,此后,随再灌注时间的延长而减少。 结论:利多卡因复合氯胺酮可减少和降低脑缺血/再灌注后大鼠神经细胞坏死和凋亡的发生。
Other AbstractObjective: To investigate the effect of lidocaine combined ketamine to the cells necrosis and apoptosis in the CA1 region of rat hippocampus following global cerebral ischemia-reperfusion. Methods: Sixty adult Wistar rats were randomly divided into 6 groups: groupⅠcontrol (n = 4), groupⅡsham operation (n = 4), group Ⅲ Model (n = 4), group Ⅳ lidocaine (n = 16), group Ⅴ ketamine (n = 16), group Ⅵ combined of lidocaine combined ketamine (n = 16). The global cerebral ischemia (10min) was induced by the use of the four-vessel occlusion method. Group Ⅳ,Ⅴ,Ⅵ intraperitoneal the lidocaine 10mg/kg, ketamine 10mg/kg, lidocaine combined ketamine 10mg/kg. The effect of cells necrosis and apoptosis was detected by using HE staining and TUNEL methods to ccmpare and analysis. Result: Compared with groupⅡthe numbers of ischemia neuron of group Ⅳ,Ⅴ,Ⅵ have significant deviation (P<0.05)in 24h, and group Ⅵ have significant decrease than group Ⅳ,Ⅴ(P<0.05). The ischemia neurons peak present in 24h. Compared with groupⅡthe numbers of apoptosis of group Ⅳ,Ⅴ,Ⅵ have significant deviation (P<0.05)in 24h , and group Ⅵ have significant decrease than group Ⅳ,Ⅴ(P<0.05). The apoptosis peak present in 24h and 48h, and decrease with reperfusion time prlong. Conclusion: The lidocaine combined ketamine can reduced the cell necrosis and apoptosis after global crebral ischemia-reperfusion in rats hippocampus.
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223075
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
张全云. 利多卡因复合氯胺酮对全脑缺血大鼠海马细胞凋亡的影响[D]. 兰州. 兰州大学,2009.
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