| 兰州地区婴幼儿急性下呼吸道感染与人偏肺病毒相关性研究 |
| Alternative Title | The relevance study of infants with acute lower respiratory tract infection and human metapneumovirus in Lanzhou，China
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| 史延 |
| Thesis Advisor | 王爱华
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| 2014-05-27
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 人偏肺病毒
婴幼儿
急性下呼吸道感染
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| Abstract | 目的：了解兰州地区婴幼儿急性下呼吸道感染患儿人类偏肺病毒(Human Metapneumocirus, hMPV)分布情况。
方法：收集2008年12月~2009年3月急性下呼吸道感染患儿咽拭子洗液标本202份，采用荧光检测及RT-PCR扩增分别检测hMPV，并进行统计学分析。
结果：剔除不合格标本及早期污染标本后，88份咽拭子洗液标本中共检测到17份RT-PCR阳性扩增产物，阳性率为19.2%（17/88）。分析认为阳性与年龄无统计学意义（P=0.693），与性别无统计学意义（P=0.719），年龄与性别有相关性，但是相关性很低（R=0.167；P=0.486）；9份荧光阳性产物，阳性率10.23%（9/88），分析认为阳性与年龄无统计学意义（P=1.000），与性别无统计学意义（P=0.663），年龄与性别有相关性，但是相关性很低（R=0.100；P=0.764）。
结论：（1）兰州地区急性呼吸道感染患儿中存在hMPV 感染; (2) hMPV阳性分布与性别分布均无关；年龄与性别低度相关。
关键词：人偏肺病毒，婴幼儿，急性下呼吸道感染 |
| Other Abstract | Objective :To understand the distribution of Lanzhou infants with acute respiratory tract infection and Human Metapneumoviru（HMPV）.
Methods :The author collected 202 throat swab lavage specimens of infants with acute lower respiratory tract infection from December 2008 to March 2009, and respectively detected HMPV using the methods of fluoroscopic examination and RT-PCR （Reverse Transcription Polymerase Chain Reaction） amplification, and then made a statistical analysis.
Results :After removing the unqualified and early contaminated specimens,17 RT-PCR positive amplification products were detected in 88 throat swabs lavage specimens, so the positive rate is 19.2% (17/88). The statistical analysis proves that there is no statistical significance of positive and age (P=0.693), positive and gender(P=0.719), and there is a correlation of positive and age, but it is very low (R=0.167; P=0.486);There are 9 fluorescence positive products, and the positive rate is 10.23% (9/88). The analysis thinks that there is no statistical significance of positive and age (P = 1.000), positive and gender (P = 0.663), but age and gender are relevant, however, the correlation is very low (R = 0.100; P = 0.764).
Conclusion:
(1) HMPV infection in the presence of infection in children with acute respiratory Lanzhou;
(2) The methods of IF (immunofluorescence) and RT-PCR all show that there is no relationship between positive distribution and age distribution,；and there is a very low correlation of age and gender.
Keywords: Human Metapneumovirus,Infants,Acute Lower Respiratory Tract Infect |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223098
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
史延. 兰州地区婴幼儿急性下呼吸道感染与人偏肺病毒相关性研究[D]. 兰州. 兰州大学,2014.
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