| Other Abstract | Objectives: To analyze the feature of epidemiology and clinical characteristics of Group A rotaviruses (GARV), Human Bocavirus (HBoV), Human Calicivirus (HuCV), Human Astrovirus (HAstV), Enterovirus (HEV) and Human Adenovirus (HAdV) in Lanzhou area of China from July 2012 to June 2013, and to provide scientific data for the prevention and control of children diarrhea.
Methods:A total of 331 stool samples were collected from hospitalized children less than five years with acute infection diarrhea, in Department of Pediatrics, the First Hospital of Lanzhou University, between July 2012 and June 2013. Personal information and Clinical data were collected from all children. Group A rotavirus was identified by enzyme immunoassay (EIA). Rotavirus G serotype, P serotype and HBoV were confirmed by nested-PCR. HuCV, HAstV, HEV and HAdV were detected by reverse transcription-Polymerase Chain Reaction (RT-PCR) or PCR. The positive stool samples were sequenced.
Results: 1.Of the 331 stool samples, 45.32% were rotavirus-positive. GARV was the most prevalent virus. The results indicated that G9 (84.00%) has gotten the highest detection rate for the first time, followed by G3 (12.67%), G1(1.33%), G2 and G1+G9 (1.33%). No G4 was detected. P[8] accounted for the most majority (96.97%), P[4] and P[4] + P[8] type were 2 cases (1.33%), P[6] was 1 cases (0.67%).The most common G/P combination identified was G9P[8], followed by G3P[8]. The most common co-infections were Rotaviruses and Bocavirus. GARV was no obvious distribution of seasons, peaked in March. The median age was 9 months (rang, 0 month to 28 months). Of all episodes of rotavirus diarrhea, 96.0% occurred during the frist 18 months, peaking at 7-12 months. Compared with non-GARV, GARV infections can be associated with severe clinical presentation, including vomiting (66.0%), fever (67.3%), frequency of diarrhea(>5 times/day) (57.3%) and frequency of vomiting(>3 times/day) (40.4%).
2.Among the 331 stool samples, 14.8%(49/331) were bocavirus-positive. We found human bocavirus 1, 2 ,3 and 4 at respective 26 ,15,7 and 1 cases. The seasonal distribution of HBoV was throughout the year. HBoV was mostly found in children aged from 7 to 12 months with a median age was 9 months. The children under 2 years old were the most susceptible population for HBoV infection (93.88%). 71.3% of the mixed infections was detected and the majority were rotaviruses. There was no statistic difference in incidence of fever and vomitin... |
