|Alternative Title||Research on the making process of the compound tissus-engineering scaffolds of the PLA/silk fibroin and biological evaluation
|Place of Conferral||兰州
|Other Abstract||Objective Research on the making process of the compound tissus-engineering scaffolds of the PLA/silk fibroin and research its biological evaluation.Compare it to the pure PLA scaffolds.
Methods Prepare and dip the PLA scaffolds matrix into the silk fibroin solution. After that, put it into the dryer to make it a PLA/silk fibroin scaffolds. Then divid them into two group of scaffolds,one group is PLA group,and the other one is compound scaffolds .Compare the two scaffolds according to ISO-10993 standard for hematolysis test,dynamic coagulation time test, cell toxicity test，stimulation test and the pyrogen test and research them separately.
Results In the stimulation test，the two kinds of materials have equally not aroused the obvious animal skin stimulation, it shows that the experiment is in accordance with the standard. In the pyrogen test, the two scaffolds material arouses the animal temperature rising without exception under 0.2℃ and the total number of degree is under 1.0℃,therefore there is no obvious difference. In the hematolysis test, the hemolysis of the two scaffolds samples to be smaller than 5% equally, P=0.000,So we may think that the compound scaffolds resists the hemolysis is better than the PLA scaffolds.In the dynamic coagulation time test,the compound one in blood time experiment,its blood time is 37 minutes, but the PLA’s is 26 minutes. The compound scaffolds clotting the blood performance to have obviously to surpass the PLA scaffolds .During the cell toxicity test ,we may see from the cell growth curve, the compound scaffolds group's cell growth situation is obviously surpass the PLA’s group, and at the meantime the cell toxicity of the compound scaffolds is obviously smaller than the PLA scaffolds.
Conclusion The material of PLA/silk fibroin compound scaffolds has the advanced biological consistent than the simplex scaffolds.Accordingly,the PLA/silk fibroin can be used as a scaffolds matrix to transplant into the body.|
邢帅. 聚乳酸/丝素蛋白复合组织工程支架的制备及生物学性能评价[D]. 兰州. 兰州大学,2008.
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