| 脊髓损伤后Wnt-3a的表达及其对神经干细胞增殖的影响 |
| Alternative Title | The expression of Wnt-3a after spinal cord injury and the effect of it on the proliferation of neural stem cells
|
| 才礼扬 |
| Thesis Advisor | 王栓科
|
| 2011-05-20
|
| Degree Grantor | 兰州大学
|
| Place of Conferral | 兰州
|
| Degree Name | 硕士
|
| Keyword | 脊髓损伤
Wnt-3a
内源性神经干细胞
|
| Abstract | 目的：研究大鼠脊髓损伤（SCI）后Wnt-3a信号蛋白的表达及其对脊髓神经干细胞增殖的影响。方法：选用30只SD成年雌性大鼠，随机分为正常对照组（A组，n=5）和损伤组（B组，n=25）。A组不损伤脊髓，B组在大鼠T9-T10段采用Allen打击法（25g？cm）造成脊髓损伤，于造模后1d，3d，7d，14d，28d进行取材。对距离损伤中心5mm的脊髓进行免疫组织化学染色，检测Wnt-3a蛋白表达的动态变化及内源性神经干细胞的增殖，与A组比较，并统计分析两者之间的相关性。结果：A组大鼠的脊髓中央管周围和外周软膜仅有极少量的Wnt-3a蛋白的表达，而BrdU阳性细胞也并不明显，白质中几乎没有。B组大鼠损伤1d后在脊髓中央管周围和外周软膜就有BrdU阳性细胞和Wnt-3a蛋白的表达，3d后表达明显增多，7d后达到高峰，14d后逐渐下降，28天后仅有少量表达。用统计软件分析Wnt-3a蛋白的表达与Brdu的表达具有相关性。结论：大鼠脊髓损伤后Wnt-3a蛋白表达增多，促进内源性神经干细胞的增殖。 |
| Other Abstract | AIM:To investigate the effect of Wnt-3a on the proliferation of neural stem cells after spinal cord injury in rats.METHODS:Thirty SD female rats were randomly divided into two groups: the contrast group (Group A,n=5) and the injury group (Group B ,n=25). The spinal cords of Group A were not injured and the spinal cords of Group B were injured by the Allen method. To took the spinal cord of models after injured 1d,3d,7d,28d and stained them by immunohistochemistry. Tested the expression of Wnt-3a and analysed the relativity between Wnt-3a and the proliferation of neural stem cells.RESULTS:We found that at the cen- -ntral canal and the outer circumference of the spinal cord of Group A there were little Wnt-3a proteins and BrdU positive cells. But at the central canal and the outer circumference of the spinal cord of Group B there were some Wnt-3a proteins and BrdU positive cells after injured 1 day and the expression enhanced absolutely after injured 3 days. They reached the highest point or level after injured 7 days and declined gradually after injured 14 days and leaved little Wnt-3a proteins and BrdU positive cells after injured 28 days. There were some relations with the expression of Wnt-3a proteins and BrdU positive cells though the analyse of statistic.CONCLUSIONS:The expression of Wnt-3a proteins were enhanced after injury and facilitated the proliferation of neural stem cells. |
| URL | 查看原文
|
| Language | 中文
|
| Document Type | 学位论文
|
| Identifier | https://ir.lzu.edu.cn/handle/262010/223252
|
| Collection | 学院待认领
|
| Affiliation | 临床医学院
|
Recommended Citation
GB/T 7714 |
才礼扬. 脊髓损伤后Wnt-3a的表达及其对神经干细胞增殖的影响[D]. 兰州. 兰州大学,2011.
|
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
