兰州大学机构库 >学院待认领
己酮可可碱对术后痛模型大鼠脊髓胶质细胞激活的影响
Alternative TitleEffect of Pentoxiflline on the spinal cord glial activation in a rat model of postoperative pain
刘志龙
Thesis Advisor石翊飒
2011-05-17
Degree Grantor兰州大学
Place of Conferral兰州
Degree Name硕士
Keyword己酮可可碱 术后痛 星形胶质细胞 小胶质细胞
Abstract目的: 研究腹腔注射己酮可可碱对术后痛模型大鼠累计疼痛评分和机械痛阈值的影响,探讨己酮可可碱对术后疼痛模型大鼠术后疼痛的治疗作用及其对脊髓星形胶质细胞和小胶质细胞激活的影响,为治疗术后疼痛提供理论依据和适宜的治疗方法。 方法: 健康雄性SD大鼠60只,体重190~220g,采用数字表法随机随机分为2组(n=30):对照组(NS组),实验组(PTX组)。本研究选择趾部切口术后痛大鼠模型。PTX组从术前15分钟起至术后5天,每日一次腹腔注射己酮可可碱50mg/kg,生理盐水治疗组在相同时间点给予等容量生理盐水。分别于术前1天和术后1h、2h、3h、5h、1d、2d、3d、5d天腹腔注射药物后30~55分钟后,随机取6只大鼠,进行累计疼痛评分(cumulative pain score)和机械缩足反射时间(Paw withdrawal threshold ,PWT)的测定,并于术后1、2、3、5天测定结束后处死,免疫组化观察脊髓(L4-L6)星形胶质细胞标记物胶质纤维酸性蛋白(glial fibrillary acid protein,GFAP)和小胶质细胞标记物离子钙接头蛋白抗体(Ionized calcium bindingadaptor molecule-1,Iba-1)的表达。 结果: ①术后1h、2h、3h、5h、1d、2d、3d累计疼痛评分,PTX组分别为2.98±0.43、3.96±0.84、6.65±0.57、8.68±0.74、10.28±0.48、7.05±0.68、5.65±0.54,和NS组同一时间点4.06±0.67、6.09±0.18、8.82±0.24、11.08±0.56、13.12±0.23、9.56±0.78、7.82±0.67相比下降(P<0.05);②术后1h、2h、3h、5h、1d、2d、3dPWT,PTX组分别为5.84±1.60、5.25±1.17、4.47±0.67、4.90±0.48、5.25±1.17、7.60±1.85、10.49±1.62,和NS组同一时间点2.48±0.87、2.14±0.56、2.08±0.53、2.19±0.38、2.37±0.56、4.54±0.96、8.13±1.49相比增加 (P<0.05);③术后1d、2d、3d脊髓背角GFAP表达,PTX组分别为181.21±4.18、205.67±4.26、230.24±3.24和NS组同一时间点244.12±4.92、258.60±4.12、276.27±3.48相比表达下调(P<0.05)。④术后3d、5d脊髓背角Iba-1表达,PTX组分别为168.24±3.24、170.66±3.99和NS组同一时间点230.27±3.48、205.28±4.05相比表达下调(P<0.05)。 结论: 腹腔注射己酮可可碱能减轻术后痛模型大鼠的累计疼痛评分和机械缩足反射阈值,具有明显的镇痛效应,该机制可能是通过抑制脊髓星形胶质细胞和小胶质细胞的激活有关。
Other AbstractObjective: The aim of this paper is to observe the effect of intraperitoneal injection of pentoxifylline on the cumulative pain score and paw withdrawal threshold in a rat model of postoperative pain and explore the therapeutic effects of pentoxifylline on the postoperative pain and the effects of the activation of Astrocyte and Microglia in the spinal cord,which may provide theoretical basis for postoperative pain treatment. Methods: Sixty male SD rats weight 190~220g were randomly divided into 2 groups: control group (NS group) and treatment group(PTX group), in which 50mg/kg saline vehicle or pentoxifylline treatment (n=30) was given by the intraperitoneally route 15min before surgery and continued daily to day 5 operation, respectively.The animal model of postoperative pain was established by incisioning hind paw. cumulative pain score and paw withdrawal threshold were measured at 1 day before operation and then at 1, 2, 3, and 5 hours and 1, 2, 3, and 5 days after operation. Spinal cord tissue(L4-L6) samples from rats were taken for analysis of glial fibrillary acid protein(GFAP) and ionized calcium bindingadaptor molecule-1(Iba-1) expression by means of immunohistochemistry at the following times after operation: 1, 2, 3, and 5 days. Results: ①At the following times after surgery: 1,2,3,5 hours and1,2d,3 days, the cumulative pain score were 2.98±0.43,3.96±0.84,6.65±0.57,8.68±0.74,10.28±0.48,7.05±0.68 and 5.65±0.54 in the PTX group respectively, less than the NS group with 4.06±0.67,6.09±0.18, 8.82±0.24,11.08±0.56,13.12±0.23,9.56±0.78 and 7.82±0.67(P<0.05).② At the following times after surgery: 1,2,3,5 hours and 1,2 and 3 days,the PWT were 5.84±1.60,5.25±1.17,4.47±0.6,4.90±0.48,5.25±1.1,7.60±1.8 and 10.49±1.6 in the PTX group respectively, more than the NS group with2.48±0.87,2.14±0.56,2.08±0.5,2.19±0.38,2.37±0.56, 4.54±0.96 and 8.13±1.49(P<0.05).③The GFAP expression in the spinal cord tissue at the following times after surgery: 1,2 days and 3 days were 181.21±4.18,205.67±4.26 and 230.24±3.24 in the PTX group respectively, lower than the NS group with 244.12±4.9,258.60±4.12 and 276.27±3.48(P<0.05).④The Iba-1 expression in the spinal cord tissue at the following times after surgery: 3 days and 5 days were 168.24±3.24 and 170.66±3.99 in the PTX group respectively, lower than the NS group with 230.27±3.48 and 205.28±4.05(P<0.05). Conclusion: pentoxifylline can evidently attenuate the cumu...
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Language中文
Document Type学位论文
Identifierhttps://ir.lzu.edu.cn/handle/262010/223255
Collection学院待认领
Affiliation临床医学院
Recommended Citation
GB/T 7714
刘志龙. 己酮可可碱对术后痛模型大鼠脊髓胶质细胞激活的影响[D]. 兰州. 兰州大学,2011.
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