| Other Abstract | Objective：
The aim of this paper is to observe the effect of intraperitoneal injection of pentoxifylline on the cumulative pain score and paw withdrawal threshold in a rat model of postoperative pain and explore the therapeutic effects of pentoxifylline on the postoperative pain and the effects of the activation of Astrocyte and Microglia in the spinal cord,which may provide theoretical basis for postoperative pain treatment.
Methods：
Sixty male SD rats weight 190~220g were randomly divided into 2 groups: control group (NS group) and treatment group(PTX group), in which 50mg/kg saline vehicle or pentoxifylline treatment (n=30) was given by the intraperitoneally route 15min before surgery and continued daily to day 5 operation, respectively.The animal model of postoperative pain was established by incisioning hind paw. cumulative pain score and paw withdrawal threshold were measured at 1 day before operation and then at 1, 2, 3, and 5 hours and 1, 2, 3, and 5 days after operation. Spinal cord tissue(L4-L6) samples from rats were taken for analysis of glial fibrillary acid protein(GFAP) and ionized calcium bindingadaptor molecule-1(Iba-1) expression by means of immunohistochemistry at the following times after operation: 1, 2, 3, and 5 days.
Results：
①At the following times after surgery: 1,2,3,5 hours and1,2d,3 days, the cumulative pain score were 2.98±0.43,3.96±0.84,6.65±0.57,8.68±0.74,10.28±0.48,7.05±0.68 and 5.65±0.54 in the PTX group respectively, less than the NS group with 4.06±0.67,6.09±0.18, 8.82±0.24,11.08±0.56,13.12±0.23,9.56±0.78 and 7.82±0.67(P<0.05).② At the following times after surgery: 1,2,3,5 hours and 1,2 and 3 days,the PWT were 5.84±1.60,5.25±1.17,4.47±0.6,4.90±0.48,5.25±1.1,7.60±1.8 and 10.49±1.6 in the PTX group respectively, more than the NS group with2.48±0.87,2.14±0.56,2.08±0.5,2.19±0.38,2.37±0.56,
4.54±0.96 and 8.13±1.49(P<0.05).③The GFAP expression in the spinal cord tissue at the following times after surgery: 1,2 days and 3 days were 181.21±4.18,205.67±4.26 and 230.24±3.24 in the PTX group respectively, lower than the NS group with 244.12±4.9,258.60±4.12 and 276.27±3.48(P<0.05).④The Iba-1 expression in the spinal cord tissue at the following times after surgery: 3 days and 5 days were 168.24±3.24 and 170.66±3.99 in the PTX group respectively, lower than the NS group with 230.27±3.48 and 205.28±4.05(P<0.05).
Conclusion：
pentoxifylline can evidently attenuate the cumu... |
