| 急进高原大鼠肠损伤后自噬变化的初步研究 |
| Alternative Title | Preliminary study on the change of autophagy after the intestinal injury in rats acutely stressed to plateau
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| 李文祥 |
| Thesis Advisor | 张方信
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| 2014-05-16
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| Degree Grantor | 兰州大学
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| Place of Conferral | 兰州
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| Degree Name | 硕士
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| Keyword | 高原
低氧
肠损伤
自噬
Beclin1
LC3B
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| Abstract | 目的 通过建立模拟急进高原大鼠模型，利用HE染色、透射电镜及免疫组化的方法，初步探讨急进高原大鼠肠损伤后细胞自噬的变化情况，为进一步研究自噬在急进高原相关肠道疾病中的作用提供一定理论依据。
方法 将50只Wistar大鼠随机分成5组：平原对照组，急进高原6 h、12 h、24 h、48 h组，每组各10只。通过低压低氧动物实验舱模拟急进海拔4767 m，建立急进高原大鼠模型。用光镜观察各组大鼠肠组织病理改变，用透射电镜观察肠上皮细胞中自噬体结构，用免疫组化法检测自噬相关蛋白Beclin1及LC3B的表达。
结果 光镜显示：与平原对照组比较，急进高原6 h组大鼠肠组织发生轻度损伤，急进高原12 h组及24 h组损伤明显加重，急进高原48 h组损伤最重。透射电镜显示：急进高原6 h组开始发现双层膜结构的自噬体，急进高原24 h组自噬体增多最明显，急进高原48 h组仍可见自噬体。免疫组化显示：Beclin1在平原对照组，急进高原6 h、12 h、24 h、48 h组的表达分别为（0.142±0.005）、（0.161±0.017）、（0.179±0.018）、（0.195±0.015）、（0.185±0.020）；急进高原6 h、12 h、24 h、48 h组Beclin1表达均显著高于平原对照组（P值均<0.01）。LC3B在平原对照组，急进高原6 h、12 h、24 h、48 h组的表达分别为（0.150±0.007）、（0.171±0.015）、（0.190±0.017）、（0.207±0.016）、（0.193±0.019）；急进高原6 h、12 h、24 h、48 h组LC3B表达均显著高于平原对照组（P值均<0.01）。
结论 1.急进高原环境可使大鼠肠组织发生不同程度的损伤，并且随着急进高原暴露时间延长，肠损伤程度逐渐加重。2.急进高原大鼠肠损伤后细胞自噬表达水平明显增强。 |
| Other Abstract | Objective Establish the model rats acutely stressed to plateau. To investigate the change of autophagy after the intestinal injury by hematoxylin-eosin staining, transmission electron microscopy and immunohistochemistry, and the result may provide theoretical basis for the role of autophagy with intestinal disorders in plateau area in further research.
Methods Fifty Wistar rats were randomly divided into five groups: the plain control group, the acutely plateau 6 h, 12 h, 24 h and 48 h group, each consisting of 10 rats. The acutely plateau groups were established the model rats exposed to hypobaric hypoxia at a simulated altitude of 4767 m by decompression chamber. The injury of the intestinal was observed by light microscopy. The autophagosome in the intestinal epithelial cells were observed by transmission electron microscopy. The expression of Beclin1 and LC3B protein were analyzed by immunohistochemistry.
Results Light microscopy shows: the intestinal mucosa may mild damage in the acutely plateau 6 h group compared with the plain control group, and the damage obvious aggravate in the acutely plateau 12 h and 24 h group. It was the most serious in the acutely plateau 48 h group. Transmission electron microscopy shows: the double membrane structure of autophagosome began to find in the acutely plateau 6 h group, and increased the most obviously in the acutely plateau 24 h group. It is still observed in the acutely plateau 48 h group. Immunohistochemistry shows: the expression of Beclin1 in the plain control group, the acutely plateau 6 h, 12 h, 24 h and 48 h group were (0.142±0.005), (0.161±0.017), (0.179±0.018), (0.195±0.015), (0.185±0.020), and the expression of Beclin1 in the acutely plateau 6 h, 12 h, 24 h and 48 h group were significantly higher than that in the plain control group (P<0.01). The expression of LC3B in the plain control group, the acutely plateau 6 h, 12 h, 24 h and 48 h group were (0.150±0.007), (0.171±0.015), (0.190±0.017), (0.207±0.016), (0.193±0.019), and the expression of LC3B in the acutely plateau 6 h, 12 h, 24 h and 48 h group were significantly higher than that in the plain control group (P<0.01).
Conclusion 1. The intestine may damage differently after the model rats acutely stressed to plateau, and the damage aggravate gradually with the exposure time extension of the acutely stressed to plateau. 2. The expression of autophagy increased after the intestinal injury in model rats acutely stressed to plateau. |
| URL | 查看原文
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| Language | 中文
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| Document Type | 学位论文
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| Identifier | https://ir.lzu.edu.cn/handle/262010/223274
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| Collection | 学院待认领
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| Affiliation | 临床医学院
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Recommended Citation
GB/T 7714 |
李文祥. 急进高原大鼠肠损伤后自噬变化的初步研究[D]. 兰州. 兰州大学,2014.
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